B7J3R6 (SYR_ACIF2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arginine--tRNA ligase
EC=6.1.1.19

Alternative name(s):
Arginyl-tRNA synthetase
Short name=ArgRS

Gene names

Name:	argS
Ordered Locus Names:	AFE_0170

Organism

Acidithiobacillus ferrooxidans (strain ATCC 23270 / DSM 14882 / NCIB 8455) (Ferrobacillus ferrooxidans (strain ATCC 23270)) [Complete proteome] [HAMAP]

Taxonomic identifier

243159 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Acidithiobacillales › Acidithiobacillaceae › Acidithiobacillus

Protein attributes

Sequence length	579 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg). HAMAP MF_00123
Subunit structure	Monomer By similarity. HAMAP MF_00123
Subcellular location	Cytoplasm By similarity HAMAP MF_00123.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW arginine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 579	579	Arginine--tRNA ligase HAMAP MF_00123		PRO_1000198863
Regions
	Motif	127 – 137	11	"HIGH" region HAMAP MF_00123

Sequences

	Sequence		Length	Mass (Da)
	B7J3R6 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 733F5D6CCB2118DC	FASTA	579	64,520
	10 20 30 40 50 60 MKAFVSQALQ GALQQLHAQG RIPGIPATLE LDRPKQVEHG HLASNVALLL AKAVGRKPRD 70 80 90 100 110 120 IADDIVAALP ASDWIARTEI AGPGFINFFL QPAAFHAVIH RVRTEKEHFG ANRNGAGQRL 130 140 150 160 170 180 QMEFVSANPT GPLHVGHGRG AAYGASLANI LRFNGFDIFC EYYVNDAGRQ MDILAASVYL 190 200 210 220 230 240 RYLEADKALP WPFPENGYRG DYVREIAAHL REQVGDRLRH AAVGLPNLPQ MSDGDIAIDT 250 260 270 280 290 300 LIAHLKQSLG EDYRTLHSAG LDEILADIRD DLEGFGVHYE RWYSEGSLMD TGAVDSAVAA 310 320 330 340 350 360 LEKAGHCYTQ EGALWFRATA FDDDKDRVLR RDNGAYTYFA SDVAYHAEKF ARGFTHVIDM 370 380 390 400 410 420 WGADHHGYVP RVKAALRALG LDDQQLEVVL VQFAILYRGT EKISMSTRAG EFVTLRELRE 430 440 450 460 470 480 EVGNDAARFF YVLRRADQHL DFDLELAKKH SEENPVFYIQ YAHARVYSLL RQSVEKGLSL 490 500 510 520 530 540 PPADGVGLEI LQESREIALA DALWRFPEVV ATAARDREPH QIAFYLRELA AAFHTYYNST 550 560 570 RILVEETPLR HARLTLCLAV AQSIANGLRL LGVSAPEQM

References

[1]

"Acidithiobacillus ferrooxidans metabolism: from genome sequence to industrial applications." Valdes J., Pedroso I., Quatrini R., Dodson R.J., Tettelin H., Blake R. II, Eisen J.A., Holmes D.S. BMC Genomics 9:597-597(2008) [PubMed: 19077236] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 23270 / DSM 14882 / NCIB 8455.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001219 Genomic DNA. Translation: ACK78518.1.
RefSeq	YP_002424679.1. NC_011761.1.
3D structure databases
ProteinModelPortal	B7J3R6.
ModBase	Search...
Protein-protein interaction databases
STRING	B7J3R6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7134449.
GenomeReviews	Gene locus AFE_0170 in contig CP001219_GR.
KEGG	afr:AFE_0170.
PATRIC	20651679. VBIAciFer29821_0164.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG695395.
OMA	RMKAAMK.
ProtClustDB	PRK01611.
Family and domain databases
HAMAP	MF_00123. Arg_tRNA_synth. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR001278. Arg-tRNA-synth_Ia. IPR015945. Arg-tRNA-synth_Ia_core. IPR005148. Arg-tRNA-synth_N. IPR008909. DALR_anticod-bd. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.30.1360.70. Arg-tRNA-synth_Ic_N. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01887.
PANTHER	PTHR11956. Arg_tRNA-synt_1c. 1 hit.
Pfam	PF03485. Arg_tRNA_synt_N. 1 hit. PF05746. DALR_1. 1 hit. PF00750. tRNA-synt_1d. 1 hit. [Graphical view]
PRINTS	PR01038. TRNASYNTHARG.
SMART	SM01016. Arg_tRNA_synt_N. 1 hit. SM00836. DALR_1. 1 hit. [Graphical view]
SUPFAM	SSF55190. Arg-tRNA-synth_Ic_N. 1 hit. SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00456. ArgS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYR_ACIF2

Accession

Primary (citable) accession number: B7J3R6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	January 25, 2012
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program