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B7J3R1

- PDXA_ACIF2

UniProt

B7J3R1 - PDXA_ACIF2

Protein

4-hydroxythreonine-4-phosphate dehydrogenase

Gene

pdxA

Organism
Acidithiobacillus ferrooxidans (strain ATCC 23270 / DSM 14882 / NCIB 8455) (Ferrobacillus ferrooxidans (strain ATCC 23270))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 45 (01 Oct 2014)
      Sequence version 1 (10 Feb 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the NAD(P)-dependent oxidation of 4-(phosphohydroxy)-L-threonine (HTP) into 2-amino-3-oxo-4-(phosphohydroxy)butyric acid which spontaneously decarboxylates to form 3-amino-2-oxopropyl phosphate (AHAP).UniRule annotation

    Catalytic activityi

    4-phosphonooxy-L-threonine + NAD+ = 3-amino-2-oxopropyl phosphate + CO2 + NADH.

    Cofactori

    Binds 1 divalent metal cation per subunit. Can use ions such as zinc, magnesium or cobalt.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei136 – 1361SubstrateUniRule annotation
    Binding sitei137 – 1371SubstrateUniRule annotation
    Metal bindingi166 – 1661Divalent metal cation; shared with dimeric partnerUniRule annotation
    Metal bindingi211 – 2111Divalent metal cation; shared with dimeric partnerUniRule annotation
    Metal bindingi266 – 2661Divalent metal cation; shared with dimeric partnerUniRule annotation
    Binding sitei274 – 2741SubstrateUniRule annotation
    Binding sitei283 – 2831SubstrateUniRule annotation
    Binding sitei292 – 2921SubstrateUniRule annotation

    GO - Molecular functioni

    1. 4-hydroxythreonine-4-phosphate dehydrogenase activity Source: UniProtKB-HAMAP
    2. cobalt ion binding Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP
    4. NAD binding Source: InterPro
    5. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. pyridoxal phosphate biosynthetic process Source: UniProtKB-HAMAP
    2. pyridoxine biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Pyridoxine biosynthesis

    Keywords - Ligandi

    Cobalt, Magnesium, Metal-binding, NAD, NADP, Zinc

    Enzyme and pathway databases

    BioCyciAFER243159:GH3S-165-MONOMER.
    UniPathwayiUPA00244; UER00312.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    4-hydroxythreonine-4-phosphate dehydrogenaseUniRule annotation (EC:1.1.1.262UniRule annotation)
    Alternative name(s):
    4-(phosphohydroxy)-L-threonine dehydrogenaseUniRule annotation
    Gene namesi
    Name:pdxAUniRule annotation
    Ordered Locus Names:AFE_0165
    OrganismiAcidithiobacillus ferrooxidans (strain ATCC 23270 / DSM 14882 / NCIB 8455) (Ferrobacillus ferrooxidans (strain ATCC 23270))
    Taxonomic identifieri243159 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAcidithiobacillalesAcidithiobacillaceaeAcidithiobacillus
    ProteomesiUP000001362: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 3333334-hydroxythreonine-4-phosphate dehydrogenasePRO_1000128234Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi243159.AFE_0165.

    Structurei

    3D structure databases

    ProteinModelPortaliB7J3R1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PdxA family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1995.
    HOGENOMiHOG000221592.
    KOiK00097.
    OMAiDTLFQDK.
    OrthoDBiEOG6GN6ZC.

    Family and domain databases

    Gene3Di3.40.718.10. 1 hit.
    HAMAPiMF_00536. PdxA.
    InterProiIPR024084. IsoPropMal-DH-like_dom.
    IPR005255. PdxA.
    [Graphical view]
    PfamiPF04166. PdxA. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00557. pdxA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B7J3R1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MITEPRLLLT VGEPAGIGPD ICLQLAFHAL PSGVLLIGDL HCLRSRALTL    50
    GLSLRLEPWL EGNPWPALER GVLHVLDVPL AQPCRPGRLD MANAPAVLAT 100
    LDKAMHLLRA GAADALVTAP VHKGIINDAG IPFTGHTEYL AAACGSPKVV 150
    MLLAGRGLRV ALATTHLPLA QVAAAVTQEG LEGTLRILHR ALREDFALSE 200
    PRILVAGLNP HAGEGGHLGH EEQDVIAPVI AALQGESLRI SGPWPADTLF 250
    TPRLLEDADA VLAMYHDQGL PVLKYHAFGE AVNITLGLPI VRTSVDHGTA 300
    LDIAGTGRAE GGSLLRALDN AAEIVRNRRA AGC 333
    Length:333
    Mass (Da):35,007
    Last modified:February 10, 2009 - v1
    Checksum:i0EE262B70D2090C7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001219 Genomic DNA. Translation: ACK80626.1.
    RefSeqiWP_009563457.1. NC_011761.1.
    YP_002424674.1. NC_011761.1.

    Genome annotation databases

    EnsemblBacteriaiACK80626; ACK80626; AFE_0165.
    GeneIDi7134988.
    KEGGiafr:AFE_0165.
    PATRICi20651669. VBIAciFer29821_0159.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001219 Genomic DNA. Translation: ACK80626.1 .
    RefSeqi WP_009563457.1. NC_011761.1.
    YP_002424674.1. NC_011761.1.

    3D structure databases

    ProteinModelPortali B7J3R1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243159.AFE_0165.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACK80626 ; ACK80626 ; AFE_0165 .
    GeneIDi 7134988.
    KEGGi afr:AFE_0165.
    PATRICi 20651669. VBIAciFer29821_0159.

    Phylogenomic databases

    eggNOGi COG1995.
    HOGENOMi HOG000221592.
    KOi K00097.
    OMAi DTLFQDK.
    OrthoDBi EOG6GN6ZC.

    Enzyme and pathway databases

    UniPathwayi UPA00244 ; UER00312 .
    BioCyci AFER243159:GH3S-165-MONOMER.

    Family and domain databases

    Gene3Di 3.40.718.10. 1 hit.
    HAMAPi MF_00536. PdxA.
    InterProi IPR024084. IsoPropMal-DH-like_dom.
    IPR005255. PdxA.
    [Graphical view ]
    Pfami PF04166. PdxA. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00557. pdxA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Acidithiobacillus ferrooxidans metabolism: from genome sequence to industrial applications."
      Valdes J., Pedroso I., Quatrini R., Dodson R.J., Tettelin H., Blake R. II, Eisen J.A., Holmes D.S.
      BMC Genomics 9:597-597(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 23270 / DSM 14882 / NCIB 8455.

    Entry informationi

    Entry nameiPDXA_ACIF2
    AccessioniPrimary (citable) accession number: B7J3R1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: February 10, 2009
    Last modified: October 1, 2014
    This is version 45 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The active site is located at the dimer interface.UniRule annotation

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3