Reviewed, UniProtKB/Swiss-Prot B7J235 (LSPA_BORBZ)

Names and origin

Protein names

Recommended name:
    Lipoprotein signal peptidase
    EC=3.4.23.36
Alternative name(s):
    Prolipoprotein signal peptidase
    Signal peptidase II
      Short name=SPase II

Gene names

Name:	lspA
Ordered Locus Names:	BbuZS7_0481

Organism

Borrelia burgdorferi (strain ZS7) [Complete proteome] [HAMAP]

Taxonomic identifier

445985 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia › Borrelia burgdorferi group

Protein attributes

Sequence length	170 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This protein specifically catalyzes the removal of signal peptides from prolipoproteins By similarity.
Catalytic activity	Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains. HAMAP MF_00161
Pathway	Protein modification; lipoprotein biosynthesis (signal peptide cleavage). HAMAP MF_00161
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the peptidase A8 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Molecular function	Aspartyl protease Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 170	170	Lipoprotein signal peptidase HAMAP MF_00161		PRO_1000190795
Regions
	Transmembrane	9 – 29	21	Potential
	Transmembrane	72 – 92	21	Potential
	Transmembrane	94 – 114	21	Potential
	Transmembrane	143 – 163	21	Potential
Sites
	Active site	114	1	By similarity
	Active site	146	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7J235-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 2F831E1D5416C3B5	FASTA	170	19,976
	10 20 30 40 50 60 MSAKSKQYFN IFVFIISLIF FDQLSKYLVA KYVKLGSIYF SFFDDFFRII HVRNTGILFS 70 80 90 100 110 120 MGSNIHYSLK KIFFLAMPIF ILIFVFYLSL KERNCIARIS LLLIFSGGVG NVIDRLFRPS 130 140 150 160 170 GVVDFLDLKF YGIFGLDRWP TFNFADSYVV IGMILFLVYD FFIKRKVLNK

References

[1]	"Genome sequence of Borrelia burgdorferi strain ZS7." Fraser-Liggett C.M., Mongodin E.F., Casjens B., Dunn J., Luft B., Qiu W., Schutzer S., Ouyang S. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001205 Genomic DNA. Translation: ACK74955.1.
RefSeq	YP_002374980.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7106435.
GenomeReviews	Gene locus BbuZS7_0481 in contig CP001205_GR.
KEGG	bbz:BbuZS7_0481.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00161. [Tree]
InterPro	IPR001872. Peptidase_A8. [Graphical view]
Pfam	PF01252. Peptidase_A8. 1 hit. [Graphical view]
PRINTS	PR00781. LIPOSIGPTASE.
TIGRFAMs	TIGR00077. lspA. 1 hit.
PROSITE	PS00855. SPASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LSPA_BORBZ

Accession

Primary (citable) accession number: B7J235

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	October 13, 2009
This is version 9 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)