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B7J1H9 (SYL_BORBZ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:BbuZS7_0257
OrganismBorrelia burgdorferi (strain ZS7) [Complete proteome] [HAMAP]
Taxonomic identifier445985 [NCBI]
Taxonomic lineageBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeBorreliaBorrelia burgdorferi group

Protein attributes

Sequence length840 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 840840Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000199181

Regions

Motif44 – 5512"HIGH" region HAMAP-Rule MF_00049
Motif617 – 6215"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6201ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B7J1H9 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: 521EA46AF0095A3D

FASTA84098,217
        10         20         30         40         50         60 
MSKYEFIKIE KKWQEFWDNN KTYKVEEDPS IPKEKRLYIL DMFPYPSANG LHVGHPEGYT 

        70         80         90        100        110        120 
ATDIFGRYKL LNGFHVLHPI GFDSFGLPAE NYAIQTGTHP QKSTEENINK FKKQIKALGF 

       130        140        150        160        170        180 
AYDWDREIRT HEENYYKWTQ WIFLQLYKKG LAYVKEMPVW YCPELGTVLA NEEIIQTSDG 

       190        200        210        220        230        240 
PKSERGSYSV EKKYLRQWVL KITKYAERLL DDLEELEWPE SVKEMQRNWI GKSTGVEIEF 

       250        260        270        280        290        300 
EIEGHSDKIK VFTTRPDTIF GITYLVIAPE NKLIEKITKN NFKQNVLKYV KHEELKSDLN 

       310        320        330        340        350        360 
RTSLEKDKSG VFTGSYAFHP ITNEKIPIWV GSYVLGTYGT GAVMGVPAHD ERDFQFAKKY 

       370        380        390        400        410        420 
QLKILPVISK SGKNEILEKA FVDDGISINS PNEFNNLKNS EVKDKVIKWL TKNKKGKEKV 

       430        440        450        460        470        480 
AYKLRDWIFS RQRYWGEPIP ILFDKLGNAI PLEENDLPLK LPEIANYKPS GTGESPLSRI 

       490        500        510        520        530        540 
KDWVNVKDMG FTRETNTMPQ WAGSCWYYLR YLDPKNSKEF ANKKKIEYWM PVDLYIGGAE 

       550        560        570        580        590        600 
HTVLHLLYSR FWHKVLYDLG YVNTKEPFKK LINQGIITSF SYQKENGVLI PNDQVIEKDN 

       610        620        630        640        650        660 
KFFDKKDNKE VTQVIAKMSK SLKNVINPDD IIKEFGADSM RIYEMFMGPL TDSKPWNTKG 

       670        680        690        700        710        720 
IIGVFRFLNK IWNLREKELS KENPPREIIS ELHKVIKKVT EDTEKLNFNT AISAMMIFIN 

       730        740        750        760        770        780 
ELLKYEKNYL NIFKPFIIIL SPYAPHLAEE LWEYIGELPS LFKNSKWPKF DESLIIKDKK 

       790        800        810        820        830        840 
EIVLQINGKI KDKILLNKET GEKELKEIAM ENSKIKSNLL NKKIVKIIVI KNKLVNIVIK 

« Hide

References

[1]"Whole-genome sequences of thirteen isolates of Borrelia burgdorferi."
Schutzer S.E., Fraser-Liggett C.M., Casjens S.R., Qiu W.G., Dunn J.J., Mongodin E.F., Luft B.J.
J. Bacteriol. 193:1018-1020(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ZS7.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001205 Genomic DNA. Translation: ACK75155.1.
RefSeqYP_002374774.1. NC_011728.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING445985.BbuZS7_0257.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACK75155; ACK75155; BbuZS7_0257.
GeneID7106292.
KEGGbbz:BbuZS7_0257.
PATRIC20559953. VBIBorBur54821_0395.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycBBUR445985:GKEU-255-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_BORBZ
AccessionPrimary (citable) accession number: B7J1H9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: May 14, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries