B7J1H5 (NTPA_BORBZ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-canonical purine NTP pyrophosphatase
EC=3.6.1.19

Alternative name(s):
Non-standard purine NTP pyrophosphatase
Nucleoside-triphosphate diphosphatase
Nucleoside-triphosphate pyrophosphatase
Short name=NTPase

Gene names

Ordered Locus Names:

BbuZS7_0252

Organism

Borrelia burgdorferi (strain ZS7) [Complete proteome] [HAMAP]

Taxonomic identifier

445985 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia › Borrelia burgdorferi group

Protein attributes

Sequence length	201 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions By similarity. HAMAP MF_01405
Catalytic activity	A nucleoside triphosphate + H2O = a nucleotide + diphosphate. HAMAP MF_01405
Cofactor	Binds 1 divalent metal cation ion per subunit; can use either magnesium or manganese By similarity.
Subunit structure	Homodimer By similarity. HAMAP MF_01405
Sequence similarities	Belongs to the HAM1 NTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro nucleoside-triphosphate diphosphatase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 201	201	Non-canonical purine NTP pyrophosphatase HAMAP MF_01405		PRO_1000145481
Regions
	Region	8 – 13	6	Substrate binding By similarity
	Region	68 – 69	2	Substrate binding By similarity
Sites
	Metal binding	68	1	Magnesium or manganese By similarity
	Binding site	158	1	Substrate By similarity
	Binding site	177	1	Substrate By similarity
	Binding site	183	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7J1H5 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 199E43E0704B4575	FASTA	201	23,138
	10 20 30 40 50 60 MKTLFFATTN ENKINEVKNI LDIPNLNLIV PQNFNIKETG KTFKENSLLK AKALFEILNN 70 80 90 100 110 120 NQNVFGEDSG LCIEALNLEP GIYSKRYDTY KLCKKLSTNE KNQLILDLMK NEKNRKAYFI 130 140 150 160 170 180 CNISYISKNG QILNFEGIIK GKIALSLNDK KNYGFGYDSI FLTKNNKKLS DLTLEEKNKI 190 200 SHRGIAFLKF KKFLLKSLFN S

References

[1]

"Whole-genome sequences of thirteen isolates of Borrelia burgdorferi." Schutzer S.E., Fraser-Liggett C.M., Casjens S.R., Qiu W.G., Dunn J.J., Mongodin E.F., Luft B.J. J. Bacteriol. 193:1018-1020(2011) [PubMed: 20935092] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ZS7.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001205 Genomic DNA. Translation: ACK74711.1.
RefSeq	YP_002374770.1. NC_011728.1.
3D structure databases
ProteinModelPortal	B7J1H5.
ModBase	Search...
Protein-protein interaction databases
STRING	B7J1H5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBORT00000003370; EBBORP00000003109; EBBORG00000003370.
GeneID	7106642.
GenomeReviews	Gene locus BbuZS7_0252 in contig CP001205_GR.
KEGG	bbz:BbuZS7_0252.
PATRIC	20559943. VBIBorBur54821_0391.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000007496.
HOGENOM	HBG697237.
OMA	VEDSGFF.
ProtClustDB	PRK14825.
Family and domain databases
HAMAP	MF_01405. Non_canon_purine_NTPase. [Tree]
InterPro	IPR002637. Ham1p-like. IPR020922. Nucleoside-triphosphatase. [Graphical view]
KO	K02428.
PANTHER	PTHR11067. Ham1p_like. 1 hit.
Pfam	PF01725. Ham1p_like. 1 hit. [Graphical view]
TIGRFAMs	TIGR00042. TIGR00042. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NTPA_BORBZ

Accession

Primary (citable) accession number: B7J1H5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	January 25, 2012
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program