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B7J0S6

- SYI_BORBZ

UniProt

B7J0S6 - SYI_BORBZ

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Borrelia burgdorferi (strain ZS7)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 43 (01 Oct 2014)
      Sequence version 1 (10 Feb 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei597 – 5971ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciBBUR445985:GKEU-858-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:BbuZS7_0862
    OrganismiBorrelia burgdorferi (strain ZS7)
    Taxonomic identifieri445985 [NCBI]
    Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeBorreliaBorrelia burgdorferi group
    ProteomesiUP000006901: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10421042Isoleucine--tRNA ligasePRO_1000216248Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi445985.BbuZS7_0862.

    Structurei

    3D structure databases

    ProteinModelPortaliB7J0S6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi48 – 5811"HIGH" regionAdd
    BLAST
    Motifi594 – 5985"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiRVEHMVE.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B7J0S6-1 [UniParc]FASTAAdd to Basket

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    MFKKVENKAN FPKIEEKILK FWNDNKIFEK SIKQREGCEE FTFYDGPPFA     50
    TGLPHFGHFV PNTIKDIIPR YQTMQGKYVK RNFGWDTHGL PVEYEVEKKL 100
    GISGKYEIEN YGIENFNKEC RKIVLRYTEE WKNIILRLGR WVDFEKGYKT 150
    MDISFMESVW WVFKNLYEKG LIYESYYVLP YSPKLATPLS NFEVNLGEYK 200
    EVNDPSLTIK FKIKDKNEYL LVWTTTPWTL PSNLGIAVGQ EIEYSKIFDK 250
    TKEEILILGS KKLNSYYDDE NSYTIIEKFK GSKLEGIEYE PIFNYFLEQK 300
    DKGAFKVHTA DYVTTDDGTG IVHIAPFGEE DYKILKKHTN VDIIDPLDAE 350
    CKFTNQVKDF KGLFVKDADK KIIENLKLRN FLFKRENYLH RYPFCYRTNC 400
    PIIYRPISSW FVNVEKIKTK LLEVNEKINW MPAHLKKGRF GKWLENAKDW 450
    AISRNRFWGN PIPIWICSKT GKKICIGSKK ELENLSGQKI EDLHKDQIDK 500
    ITWPSKDGGK FIRTSEVLDC WFESGAMPYA SNHYPFTNEI NFKNIFPADF 550
    IAEGLDQTRG WFYTLTILGT ALFENTAFKN VIVNGLVLSS DGRKMSKSFK 600
    NYTDPMQVIN TFGADALRLY LIMSPVVKAD DLKYSDNGVR DVLKNIIIPI 650
    WNAYSFFTTY AIIDKFKPPK NISLAKNNNL DKWIISELES LKKILNTEID 700
    KYNLTKSIES LLEFIDKLNN WYIRRSRRRF WKSENDKDKN DAYETLYYAI 750
    KTLMILLAPF IPFITEEIYQ NLKTDEDKQS IHLNDYPKAN ENFINKTIEE 800
    KINLARKITS MARSLRSLHN IKIRMPISTI YIVTKNQNEQ NMLMEMQEII 850
    LDEINAKEMK IKANEEELIT YKAKANFKEL GKKLGKDMKA VSAEISKLKN 900
    EDIIKIINGT SYEIKVANAK HYLSLNDIIL EREEKENLKV INEESITIGI 950
    DSLITKELYL EGLTREFVRQ IQNLRKEKNF DVSDRINLYI ENSETLKEML 1000
    NKFEKYIKTE TLALNIILNK SKLEKKINLA DDIFTLIGIE KC 1042
    Length:1,042
    Mass (Da):122,247
    Last modified:February 10, 2009 - v1
    Checksum:iCA7F863EEF9DBFAE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001205 Genomic DNA. Translation: ACK74476.1.
    RefSeqiWP_002657276.1. NC_011728.1.
    YP_002375329.1. NC_011728.1.

    Genome annotation databases

    EnsemblBacteriaiACK74476; ACK74476; BbuZS7_0862.
    GeneIDi7106254.
    KEGGibbz:BbuZS7_0862.
    PATRICi20561155. VBIBorBur54821_0967.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001205 Genomic DNA. Translation: ACK74476.1 .
    RefSeqi WP_002657276.1. NC_011728.1.
    YP_002375329.1. NC_011728.1.

    3D structure databases

    ProteinModelPortali B7J0S6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 445985.BbuZS7_0862.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACK74476 ; ACK74476 ; BbuZS7_0862 .
    GeneIDi 7106254.
    KEGGi bbz:BbuZS7_0862.
    PATRICi 20561155. VBIBorBur54821_0967.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi RVEHMVE.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci BBUR445985:GKEU-858-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ZS7.

    Entry informationi

    Entry nameiSYI_BORBZ
    AccessioniPrimary (citable) accession number: B7J0S6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: February 10, 2009
    Last modified: October 1, 2014
    This is version 43 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3