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B7J0S6

- SYI_BORBZ

UniProt

B7J0S6 - SYI_BORBZ

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Protein

Isoleucine--tRNA ligase

Gene
ileS, BbuZS7_0862
Organism
Borrelia burgdorferi (strain ZS7)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei597 – 5971ATP By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBBUR445985:GKEU-858-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:BbuZS7_0862
OrganismiBorrelia burgdorferi (strain ZS7)
Taxonomic identifieri445985 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeBorreliaBorrelia burgdorferi group
ProteomesiUP000006901: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10421042Isoleucine--tRNA ligaseUniRule annotationPRO_1000216248Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi445985.BbuZS7_0862.

Structurei

3D structure databases

ProteinModelPortaliB7J0S6.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi48 – 5811"HIGH" regionUniRule annotationAdd
BLAST
Motifi594 – 5985"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiRVEHMVE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

B7J0S6-1 [UniParc]FASTAAdd to Basket

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MFKKVENKAN FPKIEEKILK FWNDNKIFEK SIKQREGCEE FTFYDGPPFA     50
TGLPHFGHFV PNTIKDIIPR YQTMQGKYVK RNFGWDTHGL PVEYEVEKKL 100
GISGKYEIEN YGIENFNKEC RKIVLRYTEE WKNIILRLGR WVDFEKGYKT 150
MDISFMESVW WVFKNLYEKG LIYESYYVLP YSPKLATPLS NFEVNLGEYK 200
EVNDPSLTIK FKIKDKNEYL LVWTTTPWTL PSNLGIAVGQ EIEYSKIFDK 250
TKEEILILGS KKLNSYYDDE NSYTIIEKFK GSKLEGIEYE PIFNYFLEQK 300
DKGAFKVHTA DYVTTDDGTG IVHIAPFGEE DYKILKKHTN VDIIDPLDAE 350
CKFTNQVKDF KGLFVKDADK KIIENLKLRN FLFKRENYLH RYPFCYRTNC 400
PIIYRPISSW FVNVEKIKTK LLEVNEKINW MPAHLKKGRF GKWLENAKDW 450
AISRNRFWGN PIPIWICSKT GKKICIGSKK ELENLSGQKI EDLHKDQIDK 500
ITWPSKDGGK FIRTSEVLDC WFESGAMPYA SNHYPFTNEI NFKNIFPADF 550
IAEGLDQTRG WFYTLTILGT ALFENTAFKN VIVNGLVLSS DGRKMSKSFK 600
NYTDPMQVIN TFGADALRLY LIMSPVVKAD DLKYSDNGVR DVLKNIIIPI 650
WNAYSFFTTY AIIDKFKPPK NISLAKNNNL DKWIISELES LKKILNTEID 700
KYNLTKSIES LLEFIDKLNN WYIRRSRRRF WKSENDKDKN DAYETLYYAI 750
KTLMILLAPF IPFITEEIYQ NLKTDEDKQS IHLNDYPKAN ENFINKTIEE 800
KINLARKITS MARSLRSLHN IKIRMPISTI YIVTKNQNEQ NMLMEMQEII 850
LDEINAKEMK IKANEEELIT YKAKANFKEL GKKLGKDMKA VSAEISKLKN 900
EDIIKIINGT SYEIKVANAK HYLSLNDIIL EREEKENLKV INEESITIGI 950
DSLITKELYL EGLTREFVRQ IQNLRKEKNF DVSDRINLYI ENSETLKEML 1000
NKFEKYIKTE TLALNIILNK SKLEKKINLA DDIFTLIGIE KC 1042
Length:1,042
Mass (Da):122,247
Last modified:February 10, 2009 - v1
Checksum:iCA7F863EEF9DBFAE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001205 Genomic DNA. Translation: ACK74476.1.
RefSeqiWP_002657276.1. NC_011728.1.
YP_002375329.1. NC_011728.1.

Genome annotation databases

EnsemblBacteriaiACK74476; ACK74476; BbuZS7_0862.
GeneIDi7106254.
KEGGibbz:BbuZS7_0862.
PATRICi20561155. VBIBorBur54821_0967.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001205 Genomic DNA. Translation: ACK74476.1 .
RefSeqi WP_002657276.1. NC_011728.1.
YP_002375329.1. NC_011728.1.

3D structure databases

ProteinModelPortali B7J0S6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 445985.BbuZS7_0862.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACK74476 ; ACK74476 ; BbuZS7_0862 .
GeneIDi 7106254.
KEGGi bbz:BbuZS7_0862.
PATRICi 20561155. VBIBorBur54821_0967.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi RVEHMVE.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci BBUR445985:GKEU-858-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ZS7.

Entry informationi

Entry nameiSYI_BORBZ
AccessioniPrimary (citable) accession number: B7J0S6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: February 10, 2009
Last modified: September 3, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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