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B7J0S6

- SYI_BORBZ

UniProt

B7J0S6 - SYI_BORBZ

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Borrelia burgdorferi (strain ZS7)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei597 – 5971ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBBUR445985:GKEU-858-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:BbuZS7_0862
OrganismiBorrelia burgdorferi (strain ZS7)
Taxonomic identifieri445985 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeBorreliaBorrelia burgdorferi group
ProteomesiUP000006901: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10421042Isoleucine--tRNA ligasePRO_1000216248Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi445985.BbuZS7_0862.

Structurei

3D structure databases

ProteinModelPortaliB7J0S6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi48 – 5811"HIGH" regionAdd
BLAST
Motifi594 – 5985"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiRVEHMVE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

B7J0S6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFKKVENKAN FPKIEEKILK FWNDNKIFEK SIKQREGCEE FTFYDGPPFA
60 70 80 90 100
TGLPHFGHFV PNTIKDIIPR YQTMQGKYVK RNFGWDTHGL PVEYEVEKKL
110 120 130 140 150
GISGKYEIEN YGIENFNKEC RKIVLRYTEE WKNIILRLGR WVDFEKGYKT
160 170 180 190 200
MDISFMESVW WVFKNLYEKG LIYESYYVLP YSPKLATPLS NFEVNLGEYK
210 220 230 240 250
EVNDPSLTIK FKIKDKNEYL LVWTTTPWTL PSNLGIAVGQ EIEYSKIFDK
260 270 280 290 300
TKEEILILGS KKLNSYYDDE NSYTIIEKFK GSKLEGIEYE PIFNYFLEQK
310 320 330 340 350
DKGAFKVHTA DYVTTDDGTG IVHIAPFGEE DYKILKKHTN VDIIDPLDAE
360 370 380 390 400
CKFTNQVKDF KGLFVKDADK KIIENLKLRN FLFKRENYLH RYPFCYRTNC
410 420 430 440 450
PIIYRPISSW FVNVEKIKTK LLEVNEKINW MPAHLKKGRF GKWLENAKDW
460 470 480 490 500
AISRNRFWGN PIPIWICSKT GKKICIGSKK ELENLSGQKI EDLHKDQIDK
510 520 530 540 550
ITWPSKDGGK FIRTSEVLDC WFESGAMPYA SNHYPFTNEI NFKNIFPADF
560 570 580 590 600
IAEGLDQTRG WFYTLTILGT ALFENTAFKN VIVNGLVLSS DGRKMSKSFK
610 620 630 640 650
NYTDPMQVIN TFGADALRLY LIMSPVVKAD DLKYSDNGVR DVLKNIIIPI
660 670 680 690 700
WNAYSFFTTY AIIDKFKPPK NISLAKNNNL DKWIISELES LKKILNTEID
710 720 730 740 750
KYNLTKSIES LLEFIDKLNN WYIRRSRRRF WKSENDKDKN DAYETLYYAI
760 770 780 790 800
KTLMILLAPF IPFITEEIYQ NLKTDEDKQS IHLNDYPKAN ENFINKTIEE
810 820 830 840 850
KINLARKITS MARSLRSLHN IKIRMPISTI YIVTKNQNEQ NMLMEMQEII
860 870 880 890 900
LDEINAKEMK IKANEEELIT YKAKANFKEL GKKLGKDMKA VSAEISKLKN
910 920 930 940 950
EDIIKIINGT SYEIKVANAK HYLSLNDIIL EREEKENLKV INEESITIGI
960 970 980 990 1000
DSLITKELYL EGLTREFVRQ IQNLRKEKNF DVSDRINLYI ENSETLKEML
1010 1020 1030 1040
NKFEKYIKTE TLALNIILNK SKLEKKINLA DDIFTLIGIE KC
Length:1,042
Mass (Da):122,247
Last modified:February 10, 2009 - v1
Checksum:iCA7F863EEF9DBFAE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001205 Genomic DNA. Translation: ACK74476.1.
RefSeqiWP_002657276.1. NC_011728.1.
YP_002375329.1. NC_011728.1.

Genome annotation databases

EnsemblBacteriaiACK74476; ACK74476; BbuZS7_0862.
GeneIDi7106254.
KEGGibbz:BbuZS7_0862.
PATRICi20561155. VBIBorBur54821_0967.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001205 Genomic DNA. Translation: ACK74476.1 .
RefSeqi WP_002657276.1. NC_011728.1.
YP_002375329.1. NC_011728.1.

3D structure databases

ProteinModelPortali B7J0S6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 445985.BbuZS7_0862.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACK74476 ; ACK74476 ; BbuZS7_0862 .
GeneIDi 7106254.
KEGGi bbz:BbuZS7_0862.
PATRICi 20561155. VBIBorBur54821_0967.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi RVEHMVE.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci BBUR445985:GKEU-858-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ZS7.

Entry informationi

Entry nameiSYI_BORBZ
AccessioniPrimary (citable) accession number: B7J0S6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: February 10, 2009
Last modified: November 26, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3