Reviewed, UniProtKB/Swiss-Prot B7J0L2 (MURG_BORBZ)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase
    EC=2.4.1.227
Alternative name(s):
    Undecaprenyl-PP-MurNAc-pentapeptide-UDPGlcNAc GlcNAc transferase

Gene names

Name:	murG
Ordered Locus Names:	BbuZS7_0795

Organism

Borrelia burgdorferi (strain ZS7) [Complete proteome] [HAMAP]

Taxonomic identifier

445985 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia › Borrelia burgdorferi group

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Catalyzes the transfer of a GlcNAc subunit on undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide (lipid intermediate I) to form undecaprenyl-pyrophosphoryl-MurNAc-(pentapeptide)GlcNAc (lipid intermediate II) By similarity.
Catalytic activity	UDP-N-acetylglucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = UDP + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol. HAMAP MF_00033
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00033
Subcellular location	Cell inner membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 28 family. MurG subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	UDP-N-acetylgalactosamine biosynthetic process Inferred from electronic annotation. Source: InterPro cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW lipid glycosylation Inferred from electronic annotation. Source: HAMAP peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Gram-negative-bacterium-type cell wall Inferred from electronic annotation. Source: InterPro extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	carbohydrate binding Inferred from electronic annotation. Source: InterPro undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 363	363	UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase HAMAP MF_00033		PRO_1000116469

Sequences

	Sequence		Length	Mass (Da)
	B7J0L2-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 1BAFA347384DB235	FASTA	363	41,118
	10 20 30 40 50 60 MSNKKIIFFT GGGTGGHVFP GISIIQKLKE FDNEIEFFWI GKKNSIEEKL IKEQDNIKFI 70 80 90 100 110 120 SIPCGKLRRY FSFKNFTDFF KVILGIIKSF YVLKKYKPQL IYATGGFVST PAIIASSLLK 130 140 150 160 170 180 IKSITHEMDL DPGLATKINS KFANNIHISF KESEKYFKNY KNIIYTGSPI RREFLNPDPK 190 200 210 220 230 240 IIKQLTQNTN KPIISILGGS LGANALNNLA LCIKKDAEIY FIHQSGKNLN DLSEKNYLRR 250 260 270 280 290 300 QFFNAEEMAS IVKFSNLIIS RAGAGAIKEF ANAGACAILI PFKKGSRGDQ IKNAKLLTNQ 310 320 330 340 350 360 NACIYIDEDE ILNINILKII KKTLKDREKI NSLKENIKKF NNKHSSTLIA KLLIKDIKET KSK

References

[1]	"Genome sequence of Borrelia burgdorferi strain ZS7." Fraser-Liggett C.M., Mongodin E.F., Casjens B., Dunn J., Luft B., Qiu W., Schutzer S., Ouyang S. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001205 Genomic DNA. Translation: ACK74993.1.
RefSeq	YP_002375265.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7106220.
GenomeReviews	Gene locus BbuZS7_0795 in contig CP001205_GR.
KEGG	bbz:BbuZS7_0795.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00033. [Tree]
InterPro	IPR006009. GlcNAc_MurG. IPR004276. Glyco_trans_28. IPR007235. Glyco_trans_28_C. [Graphical view]
Pfam	PF04101. Glyco_tran_28_C. 1 hit. PF03033. Glyco_transf_28. 1 hit. [Graphical view]
TIGRFAMs	TIGR01133. murG. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURG_BORBZ

Accession

Primary (citable) accession number: B7J0L2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	October 13, 2009
This is version 8 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)