Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Processive diacylglycerol beta-glucosyltransferase

Gene

ugtP

Organism
Bacillus cereus (strain G9842)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Processive glucosyltransferase involved in the biosynthesis of both the bilayer- and non-bilayer-forming membrane glucolipids. Is able to successively transfer up to three glucosyl residues to diacylglycerol (DAG), thereby catalyzing the formation of beta-monoglucosyl-DAG (3-O-(beta-D-glucopyranosyl)-1,2-diacyl-sn-glycerol), beta-diglucosyl-DAG (3-O-(beta-D-glucopyranosyl-beta-(1->6)-D-glucopyranosyl)-1,2-diacyl-sn-glycerol) and beta-triglucosyl-DAG (3-O-(beta-D-glucopyranosyl-beta-(1->6)-D-glucopyranosyl-beta-(1->6)-D-glucopyranosyl)-1,2-diacyl-sn-glycerol). Beta-diglucosyl-DAG is the predominant glycolipid found in Bacillales and is also used as a membrane anchor for lipoteichoic acid (LTA).UniRule annotation

Catalytic activityi

UDP-alpha-D-glucose + 1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol = 1,2-diacyl-3-O-(beta-D-glucopyranosyl-(1->6)-O-beta-D-glucopyranosyl)-sn-glycerol + UDP.
UDP-alpha-D-glucose + 1,2-diacyl-3-O-(beta-D-glucopyranosyl-(1->6)-O-beta-D-glucopyranosyl)-sn-glycerol = 1,2-diacyl-3-O-(beta-D-glucopyranosyl-(1->6)-beta-D-glucopyranosyl-(1->6)-O-beta-D-glucopyranosyl)-sn-glycerol + UDP.
UDP-glucose + 1,2-diacyl-sn-glycerol = UDP + 1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol.UniRule annotation

Pathwayi: diglucosyl-diacylglycerol biosynthesis

This protein is involved in the pathway diglucosyl-diacylglycerol biosynthesis, which is part of Glycolipid metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway diglucosyl-diacylglycerol biosynthesis and in Glycolipid metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciBCER405531:GI5L-488-MONOMER.
UniPathwayiUPA00894.

Protein family/group databases

CAZyiGT28. Glycosyltransferase Family 28.

Names & Taxonomyi

Protein namesi
Recommended name:
Processive diacylglycerol beta-glucosyltransferaseUniRule annotation (EC:2.4.1.315)
Alternative name(s):
Beta-diglucosyldiacylglycerol synthaseUniRule annotation
Short name:
Beta-DGSUniRule annotation
Short name:
DGlcDAG synthaseUniRule annotation
Short name:
Glc2-DAG synthaseUniRule annotation
Beta-gentiobiosyldiacylglycerol synthaseUniRule annotation
Beta-monoglucosyldiacylglycerol synthaseUniRule annotation
Short name:
Beta-MGSUniRule annotation
Short name:
MGlcDAG synthaseUniRule annotation
Beta-triglucosyldiacylglycerol synthaseUniRule annotation
Short name:
TGlcDAG synthaseUniRule annotation
Diglucosyl diacylglycerol synthase (1,6-linking)
Glucosyl-beta-1,6-glucosyldiacylglycerol synthaseUniRule annotation
UDP glucosyltransferaseUniRule annotation
UDP-glucose:1,2-diacylglycerol-3-beta-D-glucosyltransferaseUniRule annotation
Gene namesi
Name:ugtPUniRule annotation
Ordered Locus Names:BCG9842_B4809
OrganismiBacillus cereus (strain G9842)
Taxonomic identifieri405531 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000006744 Componenti: Chromosome

Subcellular locationi

  • Cell membrane UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 388388Processive diacylglycerol beta-glucosyltransferasePRO_1000140341Add
BLAST

Proteomic databases

PRIDEiB7IW03.

Structurei

3D structure databases

ProteinModelPortaliB7IW03.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 28 family. UgtP subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000221565.
KOiK03429.
OMAiEPAGHIV.
OrthoDBiEOG61VZFD.

Family and domain databases

HAMAPiMF_01280. Diacylglyc_glucosyltr.
InterProiIPR009695. Diacylglyc_glucosyltr_N.
IPR007235. Glyco_trans_28_C.
IPR023589. Pro_diacylglycrl_glcsylTrfase.
[Graphical view]
PfamiPF04101. Glyco_tran_28_C. 1 hit.
PF06925. MGDG_synth. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B7IW03-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKNPKVLIL TAHYGNGHVQ VAKTLEQTFR QKGIEDVIVC DLFGESHPFI
60 70 80 90 100
TDITKYLYLK SYTIGKELYR LFYYGVEKIY DKKIASWYAN FGRKRLKTLL
110 120 130 140 150
QVEKPDIVIN TFPIIAVPEL KKQTGISIPV YNVLTDFCVH KIWIHREVDR
160 170 180 190 200
YFVATDHVKE LMVDIGVPAE QIVETGIPIR SSFELKVNPD IIYNKYQLCK
210 220 230 240 250
NKKILLIVAG AHGVLGNVKE LCQSFMSVPN LQVVVVCGKN EALKQDLLSL
260 270 280 290 300
QNQNSDALKV FGYVENIDEL FRVTSCMITK PGGITLSEAA ALQVPVILYK
310 320 330 340 350
PVPGQENENA MYFEKKGAAV VIRDDSEVFA KTEALLQDDV KLLQMKEAMK
360 370 380
SIYLPEPAGH IVDAILAENH AEPRHIPIKS PALAQSFT
Length:388
Mass (Da):43,736
Last modified:February 10, 2009 - v1
Checksum:iE2F6FAEB4E75AF25
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001186 Genomic DNA. Translation: ACK95619.1.
RefSeqiWP_000594686.1. NC_011772.1.

Genome annotation databases

EnsemblBacteriaiACK95619; ACK95619; BCG9842_B4809.
KEGGibcg:BCG9842_B4809.
PATRICi18896475. VBIBacCer50903_0437.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001186 Genomic DNA. Translation: ACK95619.1.
RefSeqiWP_000594686.1. NC_011772.1.

3D structure databases

ProteinModelPortaliB7IW03.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGT28. Glycosyltransferase Family 28.

Proteomic databases

PRIDEiB7IW03.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACK95619; ACK95619; BCG9842_B4809.
KEGGibcg:BCG9842_B4809.
PATRICi18896475. VBIBacCer50903_0437.

Phylogenomic databases

HOGENOMiHOG000221565.
KOiK03429.
OMAiEPAGHIV.
OrthoDBiEOG61VZFD.

Enzyme and pathway databases

UniPathwayiUPA00894.
BioCyciBCER405531:GI5L-488-MONOMER.

Family and domain databases

HAMAPiMF_01280. Diacylglyc_glucosyltr.
InterProiIPR009695. Diacylglyc_glucosyltr_N.
IPR007235. Glyco_trans_28_C.
IPR023589. Pro_diacylglycrl_glcsylTrfase.
[Graphical view]
PfamiPF04101. Glyco_tran_28_C. 1 hit.
PF06925. MGDG_synth. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Bacillus cereus G9842."
    Dodson R.J., Durkin A.S., Rosovitz M.J., Rasko D.A., Hoffmaster A., Ravel J., Sutton G.
    Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: G9842.

Entry informationi

Entry nameiUGTP_BACC2
AccessioniPrimary (citable) accession number: B7IW03
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: November 11, 2015
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.