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B7IV46 (B7IV46_BACC2) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 27. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase HAMAP MF_02003
EC=6.1.1.5 HAMAP MF_02003
Alternative name(s):
Isoleucyl-tRNA synthetase HAMAP MF_02003
Gene names
Name:ileS1 EMBL ACK94046.1
Synonyms:ileS HAMAP MF_02003
Ordered Locus Names:BCG9842_B3131
OrganismBacillus cereus (strain G9842) [Complete proteome] [HAMAP]
Taxonomic identifier405531 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length1033 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP MF_02003

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP MF_02003

Cofactor

Zinc By similarity. HAMAP MF_02003

Subunit structure

Monomer By similarity. HAMAP MF_02003

Subcellular location

Cytoplasm By similarity HAMAP MF_02003.

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP MF_02003

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily. HAMAP MF_02003

Sequences

Sequence LengthMass (Da)Tools
B7IV46 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: 0E702C43AD8B1E96

FASTA1,033118,317
        10         20         30         40         50         60 
MKKVDVKESA VGRETRIRKQ WNEQSIFEQS IQNREGAQSF VFYEGPPTAN GLPHVGHALG 

        70         80         90        100        110        120 
RAIKDLVARY KTMAGYKVVR KAGWDTHGLP VELGVEKQLG ISGKHEIEEY GIEPFIQKCK 

       130        140        150        160        170        180 
ESVFTYEKQW REFTESIGYW VDMDDPYVTL ENPYIESVWH ILGTIHEKGL LYKGHRVSPY 

       190        200        210        220        230        240 
CPSCQTSLSS HEVAQGYKTV KDLSATVKFK VEDSENEYFL GWTTTPWTLP ANVALAVHPN 

       250        260        270        280        290        300 
MEYVKAKQEG HVYIVAKERV QEVLKENYEV LSVHKGEELL NTSYTAPFPM KEVTNGYRVI 

       310        320        330        340        350        360 
AAEFVTADSG TGLVHIAPAY GEDDYRVVQS EGLSFLHVVD EKGEYTEAVP FLKGKFVKDC 

       370        380        390        400        410        420 
DVDIVRYLAK EGLLYHKEKY EHSYPHCWRC DSPLLYYAGE SWLIRTTAIK DTFLQNNDSV 

       430        440        450        460        470        480 
TWYPDHMKHG RFGKFLENMV DWNISRNRYW GTPLNVWECE SCDHQFAPKS IAKLRKHSTK 

       490        500        510        520        530        540 
ETPEDLELHK PYVDEVQVCC EKCGGTMNRT PEVIDVWFDS GSMPFAQYHY PFENKELFEE 

       550        560        570        580        590        600 
QFPADVIAEG IDQTRGWFYS LLAVSALYTG KVPYKRVLSL GHVLDEEGQK MSKSKGNALD 

       610        620        630        640        650        660 
PVDLVDKFGA DALRWALLVD SAPWNAKRFS ERTVLEAKSK FVDTLVNVYS FYVLYANLDE 

       670        680        690        700        710        720 
YNPKETYDVK RTKLDEWVLS RLHSTTKKVR TALDDYQFTN VAREIAALVD EVSNWYVRRS 

       730        740        750        760        770        780 
RNRFWESGMN AEKAAAYETL HEVLVTISKL IAPFTPFVAE DIHLNLEGSS VHLADYPVVN 

       790        800        810        820        830        840 
ESLLQPKLEA EMDAVLQVVE LGRSNRNQHS LKVKQPLAEL VLLEHNENDM DWESYRDIVM 

       850        860        870        880        890        900 
DELNVKAFHV ELDETKYTSY QLKLNFKTAG PKFGKNVNAV NGWLKQLSQE EVQNFVSTGR 

       910        920        930        940        950        960 
AVYEATPGEE VVVTAEDVLV EKVAKSGFSN TTNGQYTVML DTNVTEELLQ EGVAREFIRA 

       970        980        990       1000       1010       1020 
VQEYRKQLNL PVNLRVDVIL DTEVELQRTL TNHKDLLEEN LLVKQFTFGH LTNKDDELSL 

      1030 
GETKLRIKLS AAN

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References

[1]"Genome sequence of Bacillus cereus G9842."
Dodson R.J., Durkin A.S., Rosovitz M.J., Rasko D.A., Hoffmaster A., Ravel J., Sutton G.
Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001186 Genomic DNA. Translation: ACK94046.1.
RefSeqYP_002445590.1. NC_011772.1.

3D structure databases

ProteinModelPortalB7IV46.
ModBaseSearch...

Protein-protein interaction databases

STRINGB7IV46.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000106788; EBBACP00000100400; EBBACG00000106781.
GeneID7183577.
GenomeReviewsGene locus BCG9842_B3131 in contig CP001186_GR.
KEGGbcg:BCG9842_B3131.
PATRIC18899790. VBIBacCer50903_2050.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00070000031886.
HOGENOMHBG577712.
OMAPYITYDN.
ProtClustDBPRK06039.

Family and domain databases

HAMAPMF_02003. Ile_tRNA_synth_type2.
[Tree]
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-synt.
IPR023586. Ile-tRNA-synt_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
Gene3DG3DSA:3.90.740.10. G3DSA:3.90.740.10. 1 hit.
G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KOK01870.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. tRNAsyn_1a_bind. 1 hit.
SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMsTIGR00392. IleS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB7IV46_BACC2
AccessionPrimary (citable) accession number: B7IV46
Entry history
Integrated into UniProtKB/TrEMBL: February 10, 2009
Last sequence update: February 10, 2009
Last modified: January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequences·References·Cross-refs·Entry info