B7IUQ3 (LSPA_BACC2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipoprotein signal peptidase
EC=3.4.23.36

Alternative name(s):
Prolipoprotein signal peptidase
Signal peptidase II
Short name=SPase II

Gene names

Name:	lspA
Ordered Locus Names:	BCG9842_B1247

Organism

Bacillus cereus (strain G9842) [Complete proteome] [HAMAP]

Taxonomic identifier

405531 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	152 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This protein specifically catalyzes the removal of signal peptides from prolipoproteins By similarity. HAMAP MF_00161
Catalytic activity	Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains. HAMAP MF_00161
Pathway	Protein modification; lipoprotein biosynthesis (signal peptide cleavage). HAMAP MF_00161
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity HAMAP MF_00161.
Sequence similarities	Belongs to the peptidase A8 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Aspartyl protease Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 152	152	Lipoprotein signal peptidase HAMAP MF_00161		PRO_1000190791
Regions
	Transmembrane	55 – 75	21	Helical; Potential
	Transmembrane	85 – 105	21	Helical; Potential
	Transmembrane	124 – 144	21	Helical; Potential
Sites
	Active site	102	1	By similarity
	Active site	129	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7IUQ3 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 41C918076D2882E3	FASTA	152	17,424
	10 20 30 40 50 60 MIYYVIALFV IAIDQISKWL IVKNMELGTS IPIIDNVLYI TSHRNRGAAW GILENKMWFF 70 80 90 100 110 120 YIITVVFVVF IVFYMKKYAK TDKLLGISLG LILGGAIGNF IDRVFRQEVV DFIHVYIFSY 130 140 150 NYPVFNIADS ALCIGVVLII IQTLLEGKKA KE

References

[1]	"Genome sequence of Bacillus cereus G9842." Dodson R.J., Durkin A.S., Rosovitz M.J., Rasko D.A., Hoffmaster A., Ravel J., Sutton G. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: G9842.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001186 Genomic DNA. Translation: ACK97200.1.
RefSeq	YP_002447458.1. NC_011772.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B7IUQ3.
Protein family/group databases
MEROPS	A08.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000103988; EBBACP00000101624; EBBACG00000103981.
GeneID	7183422.
GenomeReviews	Gene locus BCG9842_B1247 in contig CP001186_GR.
KEGG	bcg:BCG9842_B1247.
PATRIC	18903516. VBIBacCer50903_3910.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000001848.
HOGENOM	HBG724422.
OMA	SKFLILQ.
ProtClustDB	PRK00376.
Family and domain databases
HAMAP	MF_00161. LspA. [Tree]
InterPro	IPR001872. Peptidase_A8. [Graphical view]
KO	K03101.
Pfam	PF01252. Peptidase_A8. 1 hit. [Graphical view]
PRINTS	PR00781. LIPOSIGPTASE.
TIGRFAMs	TIGR00077. LspA. 1 hit.
PROSITE	PS00855. SPASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LSPA_BACC2

Accession

Primary (citable) accession number: B7IUQ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	January 25, 2012
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program