B7IUJ2 (B7IUJ2_BACC2) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518

Gene names

Name:	topA EMBL ACK94314.1
Ordered Locus Names:	BCG9842_B1312

Organism

Bacillus cereus (strain G9842) [Complete proteome] [HAMAP]

Taxonomic identifier

405531 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	692 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206 Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 RuleBase RU000518 Nucleotide-binding
Molecular function	Isomerase Topoisomerase RuleBase RU000518 SAAS SAAS005733
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	B7IUJ2 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 88F191A19780EBA1	FASTA	692	79,042
	10 20 30 40 50 60 MSDYLVIVES PSKAKTIEKY LGKKYKVVAS MGHVRDLPKS QMGIEVKNNF TPKYITIRGK 70 80 90 100 110 120 GPVLKDLKSA AKKAKKVYLA ADPDREGEAI AWHLANTLNV DVESDCRVVF NEITKDAIKE 130 140 150 160 170 180 SFKHPRAINM DLVDAQQARR ILDRLVGYNI SPLLWKKVKK GLSAGRVQSV AVRLIIEREK 190 200 210 220 230 240 EIQSFEPEEF WTIKTEFVKG KDTFEASFYG VDGEKVQLTN ETQVNEIIEQ MKDNAFSVEN 250 260 270 280 290 300 VTRKERKRNP ALPFTTSSLQ QEAARKLNMR AKKTMMLAQQ LYEGIDLGKQ GTVGLITYMR 310 320 330 340 350 360 TDSTRISETA QTEARTYITE AYGTEYIGTE KKKETKKSNA QDAHEAIRPT SVMRKPEELK 370 380 390 400 410 420 SFLSRDQLRL YKLIWERFVA SQMASAIMDT VTARLINNNV QFRASGSVVK FPGFMKVYVE 430 440 450 460 470 480 SKDDGAEEKD KMLPPLEVGE TVFSKDLEPK QHFTQPPPRY TEARLVRTLE ELGIGRPSTY 490 500 510 520 530 540 VPTLETIQKR GYVGLDNKRF VPTELGEIVI ELILEFFPEI INIEFTANME QSLDEVEEGN 550 560 570 580 590 600 ANWVKIVDDF YVGFEPRLEK AEKEMREVEI KDEPAGEDCE LCGHPMVFKM GKYGKFMACS 610 620 630 640 650 660 NFPDCRNTKP IVKEIGVTCP KCEEGQIIER RSNKKKRLFY GCGTYPECDF VSWDKPIGRK 670 680 690 CPKCEGMLVE KKLKKGVQVQ CISCDYEEEQ QM

References

[1]	"Genome sequence of Bacillus cereus G9842." Dodson R.J., Durkin A.S., Rosovitz M.J., Rasko D.A., Hoffmaster A., Ravel J., Sutton G. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001186 Genomic DNA. Translation: ACK94314.1.
RefSeq	YP_002447397.1. NC_011772.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B7IUJ2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000103793; EBBACP00000100468; EBBACG00000103786.
GeneID	7186585.
GenomeReviews	Gene locus BCG9842_B1312 in contig CP001186_GR.
KEGG	bcg:BCG9842_B1312.
PATRIC	18903394. VBIBacCer50903_3849.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00070000032133.
HOGENOM	HBG512935.
OMA	EEYWSID.
ProtClustDB	PRK05582.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 2 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B7IUJ2_BACC2

Accession

Primary (citable) accession number: B7IUJ2

Entry history

Integrated into UniProtKB/TrEMBL:	February 10, 2009
Last sequence update:	February 10, 2009
Last modified:	December 14, 2011
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)