B7INU9 (SYM_BACC2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionine--tRNA ligase
EC=6.1.1.10

Alternative name(s):
Methionyl-tRNA synthetase
Short name=MetRS

Gene names

Name:	metG
Ordered Locus Names:	BCG9842_B0067

Organism

Bacillus cereus (strain G9842) [Complete proteome] [HAMAP]

Taxonomic identifier

405531 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	544 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP MF_00098
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_00098
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00098
Subunit structure	Monomer By similarity. HAMAP MF_00098
Subcellular location	Cytoplasm By similarity HAMAP MF_00098.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW methionine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 544	544	Methionine--tRNA ligase HAMAP MF_00098		PRO_1000199276
Regions
	Motif	10 – 20	11	"HIGH" region HAMAP MF_00098
	Motif	329 – 333	5	"KMSKS" region HAMAP MF_00098
Sites
	Metal binding	141	1	Zinc By similarity
	Metal binding	144	1	Zinc By similarity
	Metal binding	153	1	Zinc By similarity
	Metal binding	156	1	Zinc By similarity
	Binding site	332	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7INU9 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 0B0CCB2A6ABE22D3	FASTA	544	62,863
	10 20 30 40 50 60 MSIFIGGAWP YANGSLHLGH IASLLPGDIL ARYYRAKGEN VLYVSGSDCN GTPIAIRAKQ 70 80 90 100 110 120 EGVTAKEIAN KYHEEFQRCF RDLGFTYDCY TRTDSEHHHE TVQKVFLRLL EEGYIYKKTV 130 140 150 160 170 180 EQAYCKTCTQ FLPDRYVEGI CPHCHEAARG DQCDACSAIL DPLDLLEKTC KLCGSTPSVE 190 200 210 220 230 240 ETEHFYFALH TFQQQIKKVV EIAKEKGTWR DNAIQLTERY VKEGLQDRVV SRDLPIGVPI 250 260 270 280 290 300 PVKGYEDKKI YVWIEAVAGY YSASKYWAEE TGKDDHEFWN SDAQTYYVHG KDNIPFHSII 310 320 330 340 350 360 WPAVLLGIGE EAIPRHIVSN EYLTVEKRKL STSKNWAVWV PDILERYNPD SIRYFLTVNA 370 380 390 400 410 420 PENRDTDFSW REFIYSHNSE LLGAYGNFVN RTLKFIEKYY DGIVPQGTIN VELKDKVEGL 430 440 450 460 470 480 YKNVGEAIEQ TTFKVALETI FDAVRFANKY FDERQPWKER EDNPVSCEET IYNCIYLIAN 490 500 510 520 530 540 FANLLEPFLP FSSERVRSTL SIVNRNWEPQ NTLPNRIDSV QPLFERIDVK QIEHEIEKLY GAAK

References

[1]	"Genome sequence of Bacillus cereus G9842." Dodson R.J., Durkin A.S., Rosovitz M.J., Rasko D.A., Hoffmaster A., Ravel J., Sutton G. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: G9842.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001186 Genomic DNA. Translation: ACK96837.1.
RefSeq	YP_002448596.1. NC_011772.1.
3D structure databases
ProteinModelPortal	B7INU9.
ModBase	Search...
Protein-protein interaction databases
STRING	B7INU9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000105706; EBBACP00000102030; EBBACG00000105699.
GeneID	7186579.
GenomeReviews	Gene locus BCG9842_B0067 in contig CP001186_GR.
KEGG	bcg:BCG9842_B0067.
PATRIC	18905876. VBIBacCer50903_5061.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00070000031891.
HOGENOM	HBG721667.
OMA	CGNQLDP.
ProtClustDB	PRK12268.
Family and domain databases
HAMAP	MF_00098. Met_tRNA_synth_type1. [Tree]
InterPro	IPR015413. aa-tRNA-synt_I. IPR001412. aa-tRNA-synth_I_CS. IPR014758. Met-tRNA_synth. IPR023458. Met-tRNA_synth_1. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01874.
Pfam	PF08264. Anticodon_1. 1 hit. PF09334. tRNA-synt_1g. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00398. MetG. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_BACC2

Accession

Primary (citable) accession number: B7INU9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program