Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

NADPH-dependent 7-cyano-7-deazaguanine reductase

Gene

queF

Organism
Bacillus cereus (strain G9842)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1).UniRule annotation

Catalytic activityi

7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH.UniRule annotation

Pathwayi: tRNA-queuosine biosynthesis

This protein is involved in the pathway tRNA-queuosine biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tRNA-queuosine biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei56Thioimide intermediateUniRule annotation1
Active sitei63Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Queuosine biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00392.

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductaseUniRule annotation (EC:1.7.1.13UniRule annotation)
Alternative name(s):
7-cyano-7-carbaguanine reductaseUniRule annotation
NADPH-dependent nitrile oxidoreductaseUniRule annotation
PreQ(0) reductaseUniRule annotation
Gene namesi
Name:queFUniRule annotation
Ordered Locus Names:BCG9842_B3946
OrganismiBacillus cereus (strain G9842)
Taxonomic identifieri405531 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000006744 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001342931 – 165NADPH-dependent 7-cyano-7-deazaguanine reductaseAdd BLAST165

Structurei

3D structure databases

ProteinModelPortaliB7IN38.
SMRiB7IN38.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni78 – 80Substrate bindingUniRule annotation3
Regioni97 – 98Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000009528.
KOiK09457.
OMAiTLECKEF.

Family and domain databases

HAMAPiMF_00818. QueF_type1. 1 hit.
InterProiIPR029500. QueF.
IPR016856. QueF_type1.
[Graphical view]
PfamiPF14489. QueF. 1 hit.
[Graphical view]
PIRSFiPIRSF027377. Nitrile_oxidored_QueF. 1 hit.
TIGRFAMsiTIGR03139. QueF-II. 1 hit.

Sequencei

Sequence statusi: Complete.

B7IN38-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTGRLDEDLK DVTLLGNQNT KYLFEYSPEI LEVFDNNHPN RDYFVKFNCP
60 70 80 90 100
EFTSLCPKTG QPDFATIYIS YIPEQKMVES KSLKLYLFSF RNHGDFHEDC
110 120 130 140 150
MNVIMNDLIK LMDPRYIEVW GKFTPRGGIS IDPYCNYGRP GTKYEQMADY
160
RMMNHDLYPE TIDNR
Length:165
Mass (Da):19,530
Last modified:February 10, 2009 - v1
Checksum:iA18635DE4C3035B1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001186 Genomic DNA. Translation: ACK98278.1.
RefSeqiWP_001986050.1. NC_011772.1.

Genome annotation databases

EnsemblBacteriaiACK98278; ACK98278; BCG9842_B3946.
KEGGibcg:BCG9842_B3946.
PATRICi18898179. VBIBacCer50903_1245.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001186 Genomic DNA. Translation: ACK98278.1.
RefSeqiWP_001986050.1. NC_011772.1.

3D structure databases

ProteinModelPortaliB7IN38.
SMRiB7IN38.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACK98278; ACK98278; BCG9842_B3946.
KEGGibcg:BCG9842_B3946.
PATRICi18898179. VBIBacCer50903_1245.

Phylogenomic databases

HOGENOMiHOG000009528.
KOiK09457.
OMAiTLECKEF.

Enzyme and pathway databases

UniPathwayiUPA00392.

Family and domain databases

HAMAPiMF_00818. QueF_type1. 1 hit.
InterProiIPR029500. QueF.
IPR016856. QueF_type1.
[Graphical view]
PfamiPF14489. QueF. 1 hit.
[Graphical view]
PIRSFiPIRSF027377. Nitrile_oxidored_QueF. 1 hit.
TIGRFAMsiTIGR03139. QueF-II. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiQUEF_BACC2
AccessioniPrimary (citable) accession number: B7IN38
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: November 2, 2016
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.