B7IMU0 (AMPA_BACC2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable cytosol aminopeptidase
EC=3.4.11.1

Alternative name(s):
Leucine aminopeptidase
Short name=LAP
EC=3.4.11.10
Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	BCG9842_B0181

Organism

Bacillus cereus (strain G9842) [Complete proteome] [HAMAP]

Taxonomic identifier

405531 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	494 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity. HAMAP MF_00181
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181 Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
Cofactor	Binds 2 manganese ions per subunit By similarity. HAMAP MF_00181
Subcellular location	Cytoplasm By similarity HAMAP MF_00181.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: InterPro metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 494	494	Probable cytosol aminopeptidase HAMAP MF_00181		PRO_1000118447
Sites
	Active site	272	1	Potential
	Active site	346	1	Potential
	Metal binding	260	1	Manganese 2 By similarity
	Metal binding	265	1	Manganese 1 By similarity
	Metal binding	265	1	Manganese 2 By similarity
	Metal binding	283	1	Manganese 2 By similarity
	Metal binding	342	1	Manganese 1 By similarity
	Metal binding	344	1	Manganese 1 By similarity
	Metal binding	344	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7IMU0 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 7B6170AADDE6D8F9	FASTA	494	53,630
	10 20 30 40 50 60 MFQVQKELAG HEVVIVALFE EEKMSSFVQE LDKAFEGQLQ VLLEEKELST KKKAISKVHS 70 80 90 100 110 120 LGKTNVKRYY FVGLGKKESY TTETLRAALG KAFKTLQVAK VQDAAILLDS FVTEKLDAID 130 140 150 160 170 180 VAHIAAEVQG LGTYELQTYK SDKKDREELE KFTAITAEDA QEIEAALTVG YVHGRATNSA 190 200 210 220 230 240 RTLVNMPPNV LTATKLAEYA VELAEKYDMD YKVLEKEEME DLGMGALLAV NQGSVEPPKM 250 260 270 280 290 300 IALIYKGKEE WTDVIGFVGK GITYDTGGYS LKPREGMVGM KGDMGGAAAV LGAMEIIGEL 310 320 330 340 350 360 RPEQNVIAVI PSTDNVVSGT AFKPDDVITS MSGKTIEVLN TDAEGRLALA DGITYAKKLG 370 380 390 400 410 420 ANYLVDVATL TGGVIVALGN YTTGAMTNNE ELFEQVLEAS METDEPIWQL PIFDRDKERV 430 440 450 460 470 480 RNSKFADLNN SPGREGHAVM AGTFLGEFAE DTPWVHLDIA GTSETKGAHD LGPAGATGAM 490 VRTLATLVER FGEE

References

[1]	"Genome sequence of Bacillus cereus G9842." Dodson R.J., Durkin A.S., Rosovitz M.J., Rasko D.A., Hoffmaster A., Ravel J., Sutton G. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: G9842.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001186 Genomic DNA. Translation: ACK93421.1.
RefSeq	YP_002448483.1. NC_011772.1.
3D structure databases
ProteinModelPortal	B7IMU0.
ModBase	Search...
Protein-protein interaction databases
STRING	B7IMU0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000103462; EBBACP00000100467; EBBACG00000103455.
GeneID	7183186.
GenomeReviews	Gene locus BCG9842_B0181 in contig CP001186_GR.
KEGG	bcg:BCG9842_B0181.
PATRIC	18905648. VBIBacCer50903_4947.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000002613.
HOGENOM	HBG742580.
OMA	NMHLMRY.
ProtClustDB	PRK00913.
Family and domain databases
HAMAP	MF_00181. Cytosol_peptidase_M17. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR023042. Peptidase_M17_cytosol_amino. IPR008283. Peptidase_M17_N. [Graphical view]
KO	K01255.
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_BACC2

Accession

Primary (citable) accession number: B7IMU0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	January 25, 2012
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program