B7IM37 (GLGB_BACC2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan branching enzyme GlgB
EC=2.4.1.18

Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE

Gene names

Name:	glgB
Ordered Locus Names:	BCG9842_B0238

Organism

Bacillus cereus (strain G9842) [Complete proteome] [HAMAP]

Taxonomic identifier

405531 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	645 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity. HAMAP MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen biosynthesis Glycogen metabolism
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 645	645	1,4-alpha-glucan branching enzyme GlgB HAMAP MF_00685		PRO_1000131808
Sites
	Active site	309	1	Nucleophile By similarity
	Active site	352	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7IM37 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 71CD79E10C6C1E3E	FASTA	645	75,888
	10 20 30 40 50 60 MSVINCEEVK RDEFHTEKYY ESYNIFGAHV VTEDEIQGVR FTVWAPHAKA MSVVGDFNEW 70 80 90 100 110 120 DYEQHKMLQV TEEGIWSLFI PHIEEGEIYK YAIETLAGDV ILKADPYAIY AEVRPNTASV 130 140 150 160 170 180 VFDIKGYEWN DKNWTRKKKK KPIYKEAMTV YELHFGSWKK KEDGTLYSYR EMVEELIPYV 190 200 210 220 230 240 VEHQFTHIEI MPLVEHPYDR SWGYQGTGYY AATSRFGTPH DLMYFVDECH KYGIGVILDW 250 260 270 280 290 300 VPGHFCKDAH GLYLFDGTPT YEYKDKDVQE NPVWGTVNFD LGKREVRNFL ISNALFWMRY 310 320 330 340 350 360 FHIDGFRVDA VANMLYWNKE GQEQSNEHAV SFLRELNEAV FAEDEDFLMT AEDSTAWPLV 370 380 390 400 410 420 TTPTYEGGLG FNYKWNMGWM NDVLKYMECA PEYRKHIHEK MTFSLLYAYS ENFILPLSHD 430 440 450 460 470 480 EVVHGKKSLL NKMPGDYWDK FAQLRLLYGY FFTHPGKKLL FMGGEFGQFD EWKDLEDLDW 490 500 510 520 530 540 NLHDFEMHRY MHDYFKELIA LYKRSKPLWQ LDHSPEGFQW IDANNNEQSI FSFIRQGDKQ 550 560 570 580 590 600 EDALVVVCNF TKATYENYKV GVPDFEYYNE ILNSDAEQYG GSGQVNKKRL KTILEPYHNQ 610 620 630 640 AAHVEITIPP FGVSILRPVK TRKGSKKQDG SKTKVRSNVT SRGKR

References

[1]	"Genome sequence of Bacillus cereus G9842." Dodson R.J., Durkin A.S., Rosovitz M.J., Rasko D.A., Hoffmaster A., Ravel J., Sutton G. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: G9842.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001186 Genomic DNA. Translation: ACK97294.1.
RefSeq	YP_002448452.1. NC_011772.1.
3D structure databases
ProteinModelPortal	B7IM37.
ModBase	Search...
Protein-protein interaction databases
STRING	B7IM37.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000105010; EBBACP00000102569; EBBACG00000105003.
GeneID	7181593.
GenomeReviews	Gene locus BCG9842_B0238 in contig CP001186_GR.
KEGG	bcg:BCG9842_B0238.
PATRIC	18905534. VBIBacCer50903_4915.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00070000031950.
HOGENOM	HBG287139.
OMA	DGTCLYE.
ProtClustDB	PRK05402.
Family and domain databases
HAMAP	MF_00685. GlgB. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz. IPR006048. A-amylase_b_C. IPR015902. Alpha_amylase. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
KO	K00700.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF13. PTHR10357:SF13. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
TIGRFAMs	TIGR01515. Branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB_BACC2

Accession

Primary (citable) accession number: B7IM37

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program