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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Bacillus cereus (strain G9842)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei579 – 5791ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBCER405531:GI5L-4865-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:BCG9842_B0386
OrganismiBacillus cereus (strain G9842)
Taxonomic identifieri405531 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
ProteomesiUP000006744 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 802802Leucine--tRNA ligasePRO_1000199179Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi405531.BCG9842_B0386.

Structurei

3D structure databases

ProteinModelPortaliB7IL05.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi40 – 5112"HIGH" regionAdd
BLAST
Motifi576 – 5805"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B7IL05-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFNHQDIEK KWQGYWEENK TFRTPDETEK PKFYALDMFP YPSGAGLHVG
60 70 80 90 100
HPEGYTATDI LSRMKRMQGY KVLHPMGWDA FGLPAEQYAL DTGNSPAEFT
110 120 130 140 150
EHNINTFRNQ IKSLGFSYDW DREVNTTDPN YYKWTQWIFL KLFEKGLAYV
160 170 180 190 200
DEVPVNWCPA LGTVLANEEI IDGKSERGGH PVERRPMRQW MLKITAYGDR
210 220 230 240 250
LLEDLDELDW PESLKDMQRN WIGRSEGAEV HFNIDGTDEK FTVFTTRPDT
260 270 280 290 300
LFGATYCVLA PEHALVAEIT TAEQKEAVEA YINAVKMKSD LERTELAKEK
310 320 330 340 350
TGVFTGAYAV NPVNGEKLPI WIADYVLATY GTGAVMAVPA HDERDYEFAS
360 370 380 390 400
VFNLPMKEVV KGGDITKEVY TGDGAHVNSA FLDGLNKEEA IAKMIEWLEV
410 420 430 440 450
TSAGNQKVTY RLRDWLFSRQ RYWGEPIPVI HWEDGTMTAV KEEELPLVLP
460 470 480 490 500
KTENIRPSGT GESPLANIDE WVNVVDPETG KKGRRETNTM PQWAGSCWYY
510 520 530 540 550
LRYIDPNNSE ALVDPEKVKQ WLPVDIYIGG AEHAVLHLLY ARFWHKVLYD
560 570 580 590 600
IGVVPTKEPF QQLFNQGMIL GENNEKMSKS KGNVVNPDDI VASHGADTLR
610 620 630 640 650
LYEMFMGPLD ASIAWSENGL DGARRFLDRV WRLFVQDNGE LSEKITDAPN
660 670 680 690 700
KELEKAYHQT VKKVTEDYAE LRFNTAISQM MVFINDAYKA ETLPKEYVEG
710 720 730 740 750
FVKMIAPVAP HIGEELWSKL GYNETITYAS WPIFDESKLV EDEVEIVVQV
760 770 780 790 800
MGKVRAKLTM SKDASKEEME QLALEAIQDQ IEGKTVRKVI VVPGKLVNVV

AN
Length:802
Mass (Da):91,365
Last modified:February 10, 2009 - v1
Checksum:i1CAAD585E9C6031B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001186 Genomic DNA. Translation: ACK97313.1.
RefSeqiYP_002448306.1. NC_011772.1.

Genome annotation databases

EnsemblBacteriaiACK97313; ACK97313; BCG9842_B0386.
KEGGibcg:BCG9842_B0386.
PATRICi18905236. VBIBacCer50903_4766.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001186 Genomic DNA. Translation: ACK97313.1.
RefSeqiYP_002448306.1. NC_011772.1.

3D structure databases

ProteinModelPortaliB7IL05.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi405531.BCG9842_B0386.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACK97313; ACK97313; BCG9842_B0386.
KEGGibcg:BCG9842_B0386.
PATRICi18905236. VBIBacCer50903_4766.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciBCER405531:GI5L-4865-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Bacillus cereus G9842."
    Dodson R.J., Durkin A.S., Rosovitz M.J., Rasko D.A., Hoffmaster A., Ravel J., Sutton G.
    Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: G9842.

Entry informationi

Entry nameiSYL_BACC2
AccessioniPrimary (citable) accession number: B7IL05
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: April 1, 2015
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.