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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Thermosipho africanus (strain TCF52B)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei549Aminoacyl-adenylateUniRule annotation1
Binding sitei593ATPUniRule annotation1
Metal bindingi878ZincUniRule annotation1
Metal bindingi881ZincUniRule annotation1
Metal bindingi898ZincUniRule annotation1
Metal bindingi901ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciTAFR484019:G1GTU-848-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:THA_833
OrganismiThermosipho africanus (strain TCF52B)
Taxonomic identifieri484019 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesFervidobacteriaceaeThermosipho
Proteomesi
  • UP000002453 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001892081 – 910Isoleucine--tRNA ligaseAdd BLAST910

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi484019.THA_833

Structurei

3D structure databases

ProteinModelPortaliB7IGT1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi57 – 67"HIGH" regionAdd BLAST11
Motifi590 – 594"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.6202 hits
3.90.740.101 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

B7IGT1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYKETLNLP STEFSMRANL VKKEPEMLKK WKEMDDYNLV LKSREGKPKF
60 70 80 90 100
VLHDGPPYAN GNIHIGTATN KILKDIVIRY KTMRGYYAPY VPGWDTHGLP
110 120 130 140 150
IEHRVSVEMG EKIKEMSPVE IRQKCKDFAL NFVNIQREQF KRLGVRGDWD
160 170 180 190 200
NPYLTLDPKY ETHILNIFKT LVKNGNVYRG NKPVYWCPTC KTALAEAEVE
210 220 230 240 250
YHDHSSPSIY VKFKLIGEEN TYVVIWTTTP WTLPANVAIA VHPEYDYVKI
260 270 280 290 300
KVDGEYWIVA EGLLNKFAAE TEINYEVVEK FKGKDLEYKK AKHPFIDRES
310 320 330 340 350
LIVLADYVTL EDGTGCVHTA PGHGAEDYLT GLKYNLPVLS PVNEEGIFTE
360 370 380 390 400
EAGKYAGLKI WDANKVIIED LEKLGALIKT QKIEHSYPHC WRCKNPIIFR
410 420 430 440 450
ATPQWFISVD KNNLREKVLE EIKKVEWHPK WGENRITAMV KERPDWTISR
460 470 480 490 500
QRVWGTPIPA IKCKHCGEVF IDEKVIDSFI TIVEKEGTDA WFKLPEKEII
510 520 530 540 550
PEDVKCPKCG HNEFEKTYDT LDVWIDSGCS FEAVIRSKGE KFPVDLYLEG
560 570 580 590 600
DDQHRGWFQS SIFMSVAHTG QAPYKSVVTH GFIKDEHGRK MSKSLGNVID
610 620 630 640 650
PKEIVDKYGA DILRLWVSSI DFFDNIRVGK NIIQQQVEVY KKIRNTLRFL
660 670 680 690 700
LGNLSNFTKN DLVEFDKLLP LDKWALGRLQ EVISQVTEHY EKYEFSKVYS
710 720 730 740 750
LINRYCTVEL SATYLDILKD RLYVEAKDSI YRRSAQTVMF YILDALIKML
760 770 780 790 800
APVLVFTTEE AYQLSPLKEF DTVHLEYWPQ VRKEFVDEKL MEEFKILFMV
810 820 830 840 850
RDDVLKALEN ARKNDIIGHS LDAKVTLNGV NEQINNVLVK YKDYLEEIFI
860 870 880 890 900
VSQVEIGEGK VKGEFANVSV EKASGEKCQR CWKYSEETGK DEEYPNTCPR
910
CAAVLKGLRK
Length:910
Mass (Da):105,389
Last modified:February 10, 2009 - v1
Checksum:i3A1050A32E0C1698
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001185 Genomic DNA Translation: ACJ75295.1
RefSeqiWP_012579823.1, NC_011653.1

Genome annotation databases

EnsemblBacteriaiACJ75295; ACJ75295; THA_833
KEGGitaf:THA_833

Similar proteinsi

Entry informationi

Entry nameiSYI_THEAB
AccessioniPrimary (citable) accession number: B7IGT1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 10, 2009
Last modified: March 28, 2018
This is version 69 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome