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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Thermosipho africanus (strain TCF52B)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei549 – 5491Aminoacyl-adenylateUniRule annotation
Binding sitei593 – 5931ATPUniRule annotation
Metal bindingi878 – 8781ZincUniRule annotation
Metal bindingi881 – 8811ZincUniRule annotation
Metal bindingi898 – 8981ZincUniRule annotation
Metal bindingi901 – 9011ZincUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciTAFR484019:GJOH-857-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:THA_833
OrganismiThermosipho africanus (strain TCF52B)
Taxonomic identifieri484019 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesFervidobacteriaceaeThermosipho
ProteomesiUP000002453 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 910910Isoleucine--tRNA ligasePRO_1000189208Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi484019.THA_833.

Structurei

3D structure databases

ProteinModelPortaliB7IGT1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi590 – 5945"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B7IGT1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYKETLNLP STEFSMRANL VKKEPEMLKK WKEMDDYNLV LKSREGKPKF
60 70 80 90 100
VLHDGPPYAN GNIHIGTATN KILKDIVIRY KTMRGYYAPY VPGWDTHGLP
110 120 130 140 150
IEHRVSVEMG EKIKEMSPVE IRQKCKDFAL NFVNIQREQF KRLGVRGDWD
160 170 180 190 200
NPYLTLDPKY ETHILNIFKT LVKNGNVYRG NKPVYWCPTC KTALAEAEVE
210 220 230 240 250
YHDHSSPSIY VKFKLIGEEN TYVVIWTTTP WTLPANVAIA VHPEYDYVKI
260 270 280 290 300
KVDGEYWIVA EGLLNKFAAE TEINYEVVEK FKGKDLEYKK AKHPFIDRES
310 320 330 340 350
LIVLADYVTL EDGTGCVHTA PGHGAEDYLT GLKYNLPVLS PVNEEGIFTE
360 370 380 390 400
EAGKYAGLKI WDANKVIIED LEKLGALIKT QKIEHSYPHC WRCKNPIIFR
410 420 430 440 450
ATPQWFISVD KNNLREKVLE EIKKVEWHPK WGENRITAMV KERPDWTISR
460 470 480 490 500
QRVWGTPIPA IKCKHCGEVF IDEKVIDSFI TIVEKEGTDA WFKLPEKEII
510 520 530 540 550
PEDVKCPKCG HNEFEKTYDT LDVWIDSGCS FEAVIRSKGE KFPVDLYLEG
560 570 580 590 600
DDQHRGWFQS SIFMSVAHTG QAPYKSVVTH GFIKDEHGRK MSKSLGNVID
610 620 630 640 650
PKEIVDKYGA DILRLWVSSI DFFDNIRVGK NIIQQQVEVY KKIRNTLRFL
660 670 680 690 700
LGNLSNFTKN DLVEFDKLLP LDKWALGRLQ EVISQVTEHY EKYEFSKVYS
710 720 730 740 750
LINRYCTVEL SATYLDILKD RLYVEAKDSI YRRSAQTVMF YILDALIKML
760 770 780 790 800
APVLVFTTEE AYQLSPLKEF DTVHLEYWPQ VRKEFVDEKL MEEFKILFMV
810 820 830 840 850
RDDVLKALEN ARKNDIIGHS LDAKVTLNGV NEQINNVLVK YKDYLEEIFI
860 870 880 890 900
VSQVEIGEGK VKGEFANVSV EKASGEKCQR CWKYSEETGK DEEYPNTCPR
910
CAAVLKGLRK
Length:910
Mass (Da):105,389
Last modified:February 10, 2009 - v1
Checksum:i3A1050A32E0C1698
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001185 Genomic DNA. Translation: ACJ75295.1.
RefSeqiWP_012579823.1. NC_011653.1.

Genome annotation databases

EnsemblBacteriaiACJ75295; ACJ75295; THA_833.
KEGGitaf:THA_833.
PATRICi23918656. VBITheAfr129358_0841.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001185 Genomic DNA. Translation: ACJ75295.1.
RefSeqiWP_012579823.1. NC_011653.1.

3D structure databases

ProteinModelPortaliB7IGT1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi484019.THA_833.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACJ75295; ACJ75295; THA_833.
KEGGitaf:THA_833.
PATRICi23918656. VBITheAfr129358_0841.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciTAFR484019:GJOH-857-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea."
    Nesboe C.L., Bapteste E., Curtis B., Dahle H., Lopez P., Macleod D., Dlutek M., Bowman S., Zhaxybayeva O., Birkeland N.-K., Doolittle W.F.
    J. Bacteriol. 191:1974-1978(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TCF52B.

Entry informationi

Entry nameiSYI_THEAB
AccessioniPrimary (citable) accession number: B7IGT1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 10, 2009
Last modified: July 22, 2015
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.