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B7IGT1

- SYI_THEAB

UniProt

B7IGT1 - SYI_THEAB

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Protein
Isoleucine--tRNA ligase
Gene
ileS, THA_833
Organism
Thermosipho africanus (strain TCF52B)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei549 – 5491Aminoacyl-adenylate By similarity
Binding sitei593 – 5931ATP By similarity
Metal bindingi878 – 8781Zinc By similarity
Metal bindingi881 – 8811Zinc By similarity
Metal bindingi898 – 8981Zinc By similarity
Metal bindingi901 – 9011Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciTAFR484019:GJOH-857-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:THA_833
OrganismiThermosipho africanus (strain TCF52B)
Taxonomic identifieri484019 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermosipho
ProteomesiUP000002453: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 910910Isoleucine--tRNA ligaseUniRule annotation
PRO_1000189208Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi484019.THA_833.

Structurei

3D structure databases

ProteinModelPortaliB7IGT1.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotation
Add
BLAST
Motifi590 – 5945"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKESHFDE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B7IGT1-1 [UniParc]FASTAAdd to Basket

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MDYKETLNLP STEFSMRANL VKKEPEMLKK WKEMDDYNLV LKSREGKPKF    50
VLHDGPPYAN GNIHIGTATN KILKDIVIRY KTMRGYYAPY VPGWDTHGLP 100
IEHRVSVEMG EKIKEMSPVE IRQKCKDFAL NFVNIQREQF KRLGVRGDWD 150
NPYLTLDPKY ETHILNIFKT LVKNGNVYRG NKPVYWCPTC KTALAEAEVE 200
YHDHSSPSIY VKFKLIGEEN TYVVIWTTTP WTLPANVAIA VHPEYDYVKI 250
KVDGEYWIVA EGLLNKFAAE TEINYEVVEK FKGKDLEYKK AKHPFIDRES 300
LIVLADYVTL EDGTGCVHTA PGHGAEDYLT GLKYNLPVLS PVNEEGIFTE 350
EAGKYAGLKI WDANKVIIED LEKLGALIKT QKIEHSYPHC WRCKNPIIFR 400
ATPQWFISVD KNNLREKVLE EIKKVEWHPK WGENRITAMV KERPDWTISR 450
QRVWGTPIPA IKCKHCGEVF IDEKVIDSFI TIVEKEGTDA WFKLPEKEII 500
PEDVKCPKCG HNEFEKTYDT LDVWIDSGCS FEAVIRSKGE KFPVDLYLEG 550
DDQHRGWFQS SIFMSVAHTG QAPYKSVVTH GFIKDEHGRK MSKSLGNVID 600
PKEIVDKYGA DILRLWVSSI DFFDNIRVGK NIIQQQVEVY KKIRNTLRFL 650
LGNLSNFTKN DLVEFDKLLP LDKWALGRLQ EVISQVTEHY EKYEFSKVYS 700
LINRYCTVEL SATYLDILKD RLYVEAKDSI YRRSAQTVMF YILDALIKML 750
APVLVFTTEE AYQLSPLKEF DTVHLEYWPQ VRKEFVDEKL MEEFKILFMV 800
RDDVLKALEN ARKNDIIGHS LDAKVTLNGV NEQINNVLVK YKDYLEEIFI 850
VSQVEIGEGK VKGEFANVSV EKASGEKCQR CWKYSEETGK DEEYPNTCPR 900
CAAVLKGLRK 910
Length:910
Mass (Da):105,389
Last modified:February 10, 2009 - v1
Checksum:i3A1050A32E0C1698
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001185 Genomic DNA. Translation: ACJ75295.1.
RefSeqiWP_012579823.1. NC_011653.1.
YP_002334636.1. NC_011653.1.

Genome annotation databases

EnsemblBacteriaiACJ75295; ACJ75295; THA_833.
GeneIDi7070253.
KEGGitaf:THA_833.
PATRICi23918656. VBITheAfr129358_0841.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001185 Genomic DNA. Translation: ACJ75295.1 .
RefSeqi WP_012579823.1. NC_011653.1.
YP_002334636.1. NC_011653.1.

3D structure databases

ProteinModelPortali B7IGT1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 484019.THA_833.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACJ75295 ; ACJ75295 ; THA_833 .
GeneIDi 7070253.
KEGGi taf:THA_833.
PATRICi 23918656. VBITheAfr129358_0841.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KESHFDE.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci TAFR484019:GJOH-857-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea."
    Nesboe C.L., Bapteste E., Curtis B., Dahle H., Lopez P., Macleod D., Dlutek M., Bowman S., Zhaxybayeva O., Birkeland N.-K., Doolittle W.F.
    J. Bacteriol. 191:1974-1978(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TCF52B.

Entry informationi

Entry nameiSYI_THEAB
AccessioniPrimary (citable) accession number: B7IGT1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 10, 2009
Last modified: September 3, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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