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B7IFB8 (B7IFB8_THEAB) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 31. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase 1 HAMAP MF_00022
EC=6.1.1.17 HAMAP MF_00022
Alternative name(s):
Glutamyl-tRNA synthetase 1 HAMAP MF_00022
Gene names
Name:gltX1 HAMAP MF_00022 EMBL ACJ74782.1
Ordered Locus Names:THA_282
OrganismThermosipho africanus (strain TCF52B) [Complete proteome] [HAMAP]
Taxonomic identifier484019 [NCBI]
Taxonomic lineageBacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermosipho

Protein attributes

Sequence length473 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022

Subunit structure

Monomer By similarity. HAMAP MF_00022

Subcellular location

Cytoplasm By similarity HAMAP MF_00022.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. HAMAP MF_00022 RuleBase RU003489

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Motif8 – 1811"HIGH" region By similarity HAMAP MF_00022
Motif250 – 2545"KMSKS" region By similarity HAMAP MF_00022

Sites

Binding site2531ATP By similarity HAMAP MF_00022

Sequences

Sequence LengthMass (Da)Tools
B7IFB8 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: BE3672829FE428EA

FASTA47355,059
        10         20         30         40         50         60 
MVRLRFAPSP TGYLHVGGAR TALFNFLYAR KMNGKFILRI EDTDLERSEK EFEEGLIESM 

        70         80         90        100        110        120 
KWLGLNWDEG PDVGGGYGPY RQSERLDIYN KYAQKLVEEG KAYKVYAYPE EIEKLREELL 

       130        140        150        160        170        180 
SQGKAPHYTR EMLESFTTPE RIKEYEEKGL KPAIYFQMPR KEYVLNDIVK GQVVFKEGTL 

       190        200        210        220        230        240 
GDFAIIRSNG VPIYNFACVI DDYLMKITHV IRGDDHLSNT VKQLAIYEAL GWNPPEFGHV 

       250        260        270        280        290        300 
SMILGPDAKK LSKRHGATSV EEFKERGYLP QAVVNFLALL GWSHPEGKEI MSLEELISAF 

       310        320        330        340        350        360 
SLDRLGKNPA IFDPQKLKWM NAEHFRHLSD EEMLEVSKPY LEKFAREEEI NERKEWFIRL 

       370        380        390        400        410        420 
LKAIKDRVEE LSQIPELVEF FFVEPENKVE LSDEVKQVYS ELVKEIENIE QWDEKNIYQA 

       430        440        450        460        470 
FKNAMKKGKV KGKDFYMNLR IVLTGREEGP ELIDIVYLLG KENIVKRIQK QLG

« Hide

References

[1]"The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea."
Nesboe C.L., Bapteste E., Curtis B., Dahle H., Lopez P., Macleod D., Dlutek M., Bowman S., Zhaxybayeva O., Birkeland N.-K., Doolittle W.F.
J. Bacteriol. 191:1974-1978(2009) [PubMed: 19124572] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001185 Genomic DNA. Translation: ACJ74782.1.
RefSeqYP_002334123.1. NC_011653.1.

3D structure databases

ProteinModelPortalB7IFB8.
ModBaseSearch...

Protein-protein interaction databases

STRINGB7IFB8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID7071921.
GenomeReviewsGene locus THA_282 in contig CP001185_GR.
KEGGtaf:THA_282.
PATRIC23917494. VBITheAfr129358_0287.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG628189.
OMAVRFRMPD.
ProtClustDBPRK01406.

Family and domain databases

HAMAPMF_00022_B. Glu_tRNA_synth_B.
[Tree]
InterProIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-synth_Ib_bac/mito.
IPR000924. Glu/Gln-tRNA-synth_Ib.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
Gene3DG3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit.
G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KOK09698.
PANTHERPTHR10119. Glu_tRNA-synt_1c. 1 hit.
PTHR10119:SF1. PTHR10119:SF1. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMsTIGR00464. GltX_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB7IFB8_THEAB
AccessionPrimary (citable) accession number: B7IFB8
Entry history
Integrated into UniProtKB/TrEMBL: February 10, 2009
Last sequence update: February 10, 2009
Last modified: January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info