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B7IE73 (NADK_THEAB) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NAD kinase

EC=2.7.1.23
Alternative name(s):
ATP-dependent NAD kinase
Gene names
Name:nadK
Ordered Locus Names:THA_1874
OrganismThermosipho africanus (strain TCF52B) [Complete proteome] [HAMAP]
Taxonomic identifier484019 [NCBI]
Taxonomic lineageBacteriaThermotogaeThermotogalesThermotogaceaeThermosipho

Protein attributes

Sequence length255 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP By similarity. HAMAP-Rule MF_00361

Catalytic activity

ATP + NAD+ = ADP + NADP+. HAMAP-Rule MF_00361

Cofactor

Divalent metal ions By similarity. HAMAP-Rule MF_00361

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00361.

Sequence similarities

Belongs to the NAD kinase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
NAD
NADP
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processNAD metabolic process

Inferred from electronic annotation. Source: InterPro

NADP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

NAD+ kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 255255NAD kinase HAMAP-Rule MF_00361
PRO_1000120892

Regions

Nucleotide binding53 – 542NAD By similarity
Nucleotide binding117 – 1182NAD By similarity
Nucleotide binding157 – 1626NAD By similarity

Sites

Active site531Proton acceptor By similarity
Binding site581NAD By similarity
Binding site1281NAD By similarity
Binding site1441NAD By similarity
Binding site1461NAD By similarity
Binding site1811NAD; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
B7IE73 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: E126E15FB5B6751A

FASTA25528,513
        10         20         30         40         50         60 
MKHLGIFYKP ELFGKAESLK ELLLNEGYIV DYCESSERIK NNKVDLTIVL GGDGTFLKAS 

        70         80         90        100        110        120 
HLVNNPLVGF KGGRLGFLSS YTVEEFDKFL KDLKNNNFVS DERTFLKVSD LNTFCLNEVL 

       130        140        150        160        170        180 
LVKDPDQKMV DIKISFQDGE LFFHADGIML STPTGSTGYS LSLGGPILLP NTKAFVITPV 

       190        200        210        220        230        240 
APQFLASRSI VIPDNEKVNI EVDKRVNLII DGVNFGKFSK ITVMKSKKKI SILRPVDYDF 

       250 
TKSIKEKLGY GKKIL 

« Hide

References

[1]"The genome of Thermosipho africanus TCF52B: lateral genetic connections to the Firmicutes and Archaea."
Nesboe C.L., Bapteste E., Curtis B., Dahle H., Lopez P., Macleod D., Dlutek M., Bowman S., Zhaxybayeva O., Birkeland N.-K., Doolittle W.F.
J. Bacteriol. 191:1974-1978(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: TCF52B.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001185 Genomic DNA. Translation: ACJ76300.1.
RefSeqYP_002335641.1. NC_011653.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING484019.THA_1874.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACJ76300; ACJ76300; THA_1874.
GeneID7070380.
KEGGtaf:THA_1874.
PATRIC23920806. VBITheAfr129358_1894.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0061.
HOGENOMHOG000227222.
KOK00858.
OMAVAPYTLY.
OrthoDBEOG6PZXDR.

Enzyme and pathway databases

BioCycTAFR484019:GJOH-1914-MONOMER.

Family and domain databases

Gene3D2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPMF_00361. NAD_kinase.
InterProIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERPTHR20275. PTHR20275. 1 hit.
PfamPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMSSF111331. SSF111331. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNADK_THEAB
AccessionPrimary (citable) accession number: B7IE73
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: July 9, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families