Reviewed, UniProtKB/Swiss-Prot B7ICE4 (MURC_ACIB5)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramate--L-alanine ligase
    EC=6.3.2.8
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	AB57_3786

Organism

Acinetobacter baumannii (strain AB0057) [Complete proteome] [HAMAP]

Taxonomic identifier

480119 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	482 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00046
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm Probable HAMAP MF_00046.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 482	482	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_1000116612
Regions
	Nucleotide binding	129 – 135	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	B7ICE4-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 4F18E9DBFCAD79ED	FASTA	482	52,882
	10 20 30 40 50 60 MSPTTAANQA KKLIKVPEMR RIKHIHFVGI GGAGMCGIAE VLANQGYKIS GSDIKASKTT 70 80 90 100 110 120 QQLEENGIKV YIGHEAENIK NANVLVVSTA IDPENPEVKA AIEQRIPIVR RAEMLGELMR 130 140 150 160 170 180 YRHGIAVAGT HGKTTTTSLL TTMLAEENLD PTYVIGGLLN STGVNAALGE SRFIVAEADE 190 200 210 220 230 240 SDASFLYLQP MAAIVTNIDA DHMDTYEGSF DKLKDTFVQF LHNLPFYGLA VVCGDDANIR 250 260 270 280 290 300 EILPRVGRPV ITYGFNEDND IRAIDVEQDG MRSHFTVLRK GREPLRLTIN QPGLHNVLNA 310 320 330 340 350 360 LAAIGVATDE GVSDEAISRA LKGFSGVGRR FQVQGEFELG EGNVKLVDDY GHHPKEVEAT 370 380 390 400 410 420 IKAARQSHPD RRLVMLFQPH RYSRTRDCFD DFIEVLSQVD QLLLLEVYPA GEKPIVGADS 430 440 450 460 470 480 RTLARSIRLR GQVEPILIDP VEGNLQNIMQ NVLQPNDLLL TQGAGNVGAI SVELAQHHLY VK

References

[1]

"Comparative genome sequence analysis of multidrug-resistant Acinetobacter baumannii." Adams M.D., Goglin K., Molyneaux N., Hujer K.M., Lavender H., Jamison J.J., MacDonald I.J., Martin K.M., Russo T., Campagnari A.A., Hujer A.M., Bonomo R.A., Gill S.R. J. Bacteriol. 190:8053-8064(2008) [PubMed: 18931120] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001182 Genomic DNA. Translation: ACJ43138.1.
RefSeq	YP_002321074.1.
3D structure databases
SMR	B7ICE4. Positions 16-476.
ModBase	Search...
Genome annotation databases
GeneID	7043767.
GenomeReviews	Gene locus AB57_3786 in contig CP001182_GR.
KEGG	abn:AB57_3786.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG476594.
OMA	YQPHRYS.
Family and domain databases
HAMAP	MF_00046. MurC. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01082. murC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_ACIB5

Accession

Primary (citable) accession number: B7ICE4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	February 9, 2010
This is version 10 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)