B7I8C4 (ASPD_ACIB5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable L-aspartate dehydrogenase
EC=1.4.1.21

Gene names

Name:	nadX
Ordered Locus Names:	AB57_1031

Organism

Acinetobacter baumannii (strain AB0057) [Complete proteome] [HAMAP]

Taxonomic identifier

480119 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter › Acinetobacter calcoaceticus/baumannii complex

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically catalyzes the NAD or NADP-dependent dehydrogenation of L-aspartate to iminoaspartate By similarity. HAMAP MF_01265
Catalytic activity	L-aspartate + H2O + NAD(P)+ = oxaloacetate + NH3 + NAD(P)H. HAMAP MF_01265
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (dehydrogenase route): step 1/1. HAMAP MF_01265
Miscellaneous	The iminoaspartate product is unstable in aqueous solution and can decompose to oxaloacetate and ammonia By similarity. HAMAP MF_01265
Sequence similarities	Belongs to the L-aspartate dehydrogenase family.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro NADP catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro aspartate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 263	263	Probable L-aspartate dehydrogenase HAMAP MF_01265		PRO_1000140082
Sites
	Active site	216	1	By similarity
	Binding site	120	1	NAD; via amide nitrogen By similarity
	Binding site	186	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7I8C4 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: A2F94A128F512144	FASTA	263	27,954
	10 20 30 40 50 60 MKKLMMIGFG AMAAEVYAHL PQDLQLKWIV VPSRSIEKVQ SQVSSDIQVI SDIEQCDGTP 70 80 90 100 110 120 DYVIEVAGQA AVKEHAQKVL AKGWTIGLIS VGTLADSEFL VQLKQTAEKN DAHLHLLAGA 130 140 150 160 170 180 IAGIDGISAA KEGGLQKVTY KGCKSPKSWK GSYAEQLVDL DHVSEPTVFF TGTAREAAMK 190 200 210 220 230 240 FPANANVAAT IALAGLGMDE TMVELTVDPT INKNKHTIVA EGGFGQMTIE LVGVPLPSNP 250 260 KTSTLAALSV IRACRNSVEA IQI

References

[1]

"Comparative genome sequence analysis of multidrug-resistant Acinetobacter baumannii." Adams M.D., Goglin K., Molyneaux N., Hujer K.M., Lavender H., Jamison J.J., MacDonald I.J., Martin K.M., Russo T., Campagnari A.A., Hujer A.M., Bonomo R.A., Gill S.R. J. Bacteriol. 190:8053-8064(2008) [PubMed: 18931120] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AB0057.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001182 Genomic DNA. Translation: ACJ40823.1.
RefSeq	YP_002318422.1. NC_011586.1.
3D structure databases
ProteinModelPortal	B7I8C4.
ModBase	Search...
Protein-protein interaction databases
STRING	B7I8C4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7045588.
GenomeReviews	Gene locus AB57_1031 in contig CP001182_GR.
KEGG	abn:AB57_1031.
PATRIC	20695882. VBIAciBau111166_1009.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG649642.
OMA	ECAGHSA.
ProtClustDB	PRK13303.
Family and domain databases
HAMAP	MF_01265. NadX. [Tree]
InterPro	IPR005106. Asp/hSer_DH_NAD-bd. IPR002811. Asp_DH. IPR011182. Asp_DH_NAD_syn. IPR020626. Asp_DH_NAD_syn_prok. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K06989.
Pfam	PF01958. DUF108. 1 hit. PF03447. NAD_binding_3. 1 hit. [Graphical view]
PIRSF	PIRSF005227. Asp_dh_NAD_syn. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ASPD_ACIB5

Accession

Primary (citable) accession number: B7I8C4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	January 25, 2012
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program