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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Acinetobacter baumannii (strain AB0057)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei77SubstrateUniRule annotation1
Metal bindingi225ManganeseUniRule annotation1
Metal bindingi245Manganese; via tele nitrogenUniRule annotation1
Binding sitei267SubstrateUniRule annotation1
Active sitei269UniRule annotation1
Metal bindingi294ManganeseUniRule annotation1
Binding sitei384GTPUniRule annotation1
Binding sitei415GTPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi268 – 273GTPUniRule annotation6
Nucleotide bindingi516 – 519GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:AB57_3085
OrganismiAcinetobacter baumannii (strain AB0057)
Taxonomic identifieri480119 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeAcinetobacterAcinetobacter calcoaceticus/baumannii complex

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001250381 – 610Phosphoenolpyruvate carboxykinase [GTP]Add BLAST610

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB7I624.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni216 – 218Substrate bindingUniRule annotation3
Regioni382 – 384Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000191700.
KOiK01596.
OMAiYGENMRV.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP. 1 hit.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B7I624-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTVNAPEFV RHPKLIAWVE EIANLTKPAK IEWCDGSEEE YQRLIDLMIA
60 70 80 90 100
NGTMQKLNQE KHPGSYLANS DPSDVARVED RTYICSQNKE DAGATNNWED
110 120 130 140 150
PAVMREKLNG LFEGSMKGRT MYVVPFSMGP LGSHIAHIGI ELTDSPYVAV
160 170 180 190 200
SMRKMARMGK AVYDVLGTDG EFVPCVHTVG APLAEGQKDV AWPCNPEKYI
210 220 230 240 250
VHYPETREIW SFGSGYGGNA LLGKKCLALR IASVMGREQG WLAEHMLILG
260 270 280 290 300
VTNPQGEKHY IAAAFPSACG KTNFAMLIPP AGYEGWKIET VGDDIAWIKP
310 320 330 340 350
GEDGRLYAIN PEAGFFGVAP GTNTKTNPNC MATLHKDVIY TNVAVTDDGQ
360 370 380 390 400
VWWEGLSKEV PANLTNWKGQ PHVNGEKAAH PNARFTVAAG QCPSIDADWE
410 420 430 440 450
NPAGVPISAF IFGGRRADTV PLVSEAFDWV DGVYKAATMG SETTAAAVGQ
460 470 480 490 500
QGIVRRDPFA MLPFAGYNMA DYFDHWLNLG AKVSEKAEAS GNKLPKIFNV
510 520 530 540 550
NWFRRDAEGN FVWPGFGQNM RVLEWIIDRC EGRANAVETP IGFVPTYEDL
560 570 580 590 600
NWEGTEFTKE QFDLITNQDR DQWVTEIESH TELFNKLGER LPKALKERQA
610
ALLEAVKTGF
Length:610
Mass (Da):67,448
Last modified:February 10, 2009 - v1
Checksum:i6FAD9D3231AD796E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001182 Genomic DNA. Translation: ACJ42417.1.
RefSeqiWP_000214356.1. NC_011586.1.

Genome annotation databases

EnsemblBacteriaiACJ42417; ACJ42417; AB57_3085.
KEGGiabn:AB57_3085.
PATRICi20699849. VBIAciBau111166_2972.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001182 Genomic DNA. Translation: ACJ42417.1.
RefSeqiWP_000214356.1. NC_011586.1.

3D structure databases

ProteinModelPortaliB7I624.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACJ42417; ACJ42417; AB57_3085.
KEGGiabn:AB57_3085.
PATRICi20699849. VBIAciBau111166_2972.

Phylogenomic databases

HOGENOMiHOG000191700.
KOiK01596.
OMAiYGENMRV.

Enzyme and pathway databases

UniPathwayiUPA00138.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP. 1 hit.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPCKG_ACIB5
AccessioniPrimary (citable) accession number: B7I624
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: November 2, 2016
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.