B7I5F5 (PROA_ACIB5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-glutamyl phosphate reductase
Short name=GPR
EC=1.2.1.41

Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	AB57_0592

Organism

Acinetobacter baumannii (strain AB0057) [Complete proteome] [HAMAP]

Taxonomic identifier

480119 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter › Acinetobacter calcoaceticus/baumannii complex

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate By similarity. HAMAP MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity HAMAP MF_00412.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 421	421	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_1000123766

Sequences

	Sequence		Length	Mass (Da)
	B7I5F5 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: BB9D19EFB27D1943	FASTA	421	45,687
	10 20 30 40 50 60 MQDSIEQYMQ KVGQQARDAS RVLTSASTSL KNHALSAIYT ALENNQAAIL AANQIDMEKG 70 80 90 100 110 120 RSNQLDSALL DRLELTPARF KGMLQGLKDV IALVDPIGEI TDLAYRPTGI QIGKMRVPLG 130 140 150 160 170 180 VVGMIYESRP NVTLEAASLA IKSGNAIILR GGSEALESNK AIAEAVKHGL KVAGLPEHSV 190 200 210 220 230 240 QVIETSDRAA VGHLITMAEY VDVIVPRGGK SLIERVTNEA RIPVIKHLDG NCHVFVEAQA 250 260 270 280 290 300 DLQKALPITL NAKTHRYGVC NAMETLLVDE KIAEVFLPHI AELYAEKQVE LRGCPETRRI 310 320 330 340 350 360 LGSSVKPATE EDWYTEYLGP ILAVKVVSGI DEAIDHINKY GSHHTDAIVT ENYTLARQFL 370 380 390 400 410 420 ARVDSSSVVV NASTRFADGF EYGLGAEIGI STDKIHARGP VGLEGLTSQK WIVLGDGQIR Q

References

[1]

"Comparative genome sequence analysis of multidrug-resistant Acinetobacter baumannii." Adams M.D., Goglin K., Molyneaux N., Hujer K.M., Lavender H., Jamison J.J., MacDonald I.J., Martin K.M., Russo T., Campagnari A.A., Hujer A.M., Bonomo R.A., Gill S.R. J. Bacteriol. 190:8053-8064(2008) [PubMed: 18931120] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AB0057.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001182 Genomic DNA. Translation: ACJ40013.1.
RefSeq	YP_002317996.1. NC_011586.1.
3D structure databases
ProteinModelPortal	B7I5F5.
SMR	B7I5F5. Positions 14-421.
ModBase	Search...
Protein-protein interaction databases
STRING	B7I5F5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7043642.
GenomeReviews	Gene locus AB57_0592 in contig CP001182_GR.
KEGG	abn:AB57_0592.
PATRIC	20694991. VBIAciBau111166_0580.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG318080.
OMA	QYPAACN.
ProtClustDB	PRK00197.
Family and domain databases
HAMAP	MF_00412. ProA. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR000965. G-glutamylP_reductase. IPR020593. G-glutamylP_reductase_CS. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 2 hits.
KO	K00147.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
Pfam	PF00171. Aldedh. 2 hits. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMs	TIGR00407. ProA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_ACIB5

Accession

Primary (citable) accession number: B7I5F5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	January 25, 2012
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program