Reviewed, UniProtKB/Swiss-Prot B7I3J1 (QUEF_ACIB5)

Names and origin

Protein names

Recommended name:
    NADPH-dependent 7-cyano-7-deazaguanine reductase
    EC=1.7.1.13
Alternative name(s):
    7-cyano-7-carbaguanine reductase
    PreQ(0) reductase
    NADPH-dependent nitrile oxidoreductase

Gene names

Name:	queF
Ordered Locus Names:	AB57_2746

Organism

Acinetobacter baumannii (strain AB0057) [Complete proteome] [HAMAP]

Taxonomic identifier

480119 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	270 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity.
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP MF_00817
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00817
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW queuosine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP queuine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 270	270	NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP MF_00817		PRO_1000193203

Sequences

	Sequence		Length	Mass (Da)
	B7I3J1-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 68A89510339FB013	FASTA	270	30,919
	10 20 30 40 50 60 MSVEQSLLGK ETQYPTSYQP DVLFPIARAQ SREKYAHIQG ITQGKDWWHV FEISWLNAHG 70 80 90 100 110 120 IPQVAIGRIT LPASSPNLIE SKSLKLYFNS LNFTQFDSTQ SFIETVEKDL SAAAGAKVEL 130 140 150 160 170 180 TLFQVDDLEI SKPQGICIDD LMPERLEQHP DATLLKLDES GEEIEVELYS HLLRSNCPVT 190 200 210 220 230 240 GQPDWGTVFI RFKGKKPCYR SLLAYIISYR QHNGFHEQCV EQIFADIWQN LQPEKLMVYA 250 260 270 TYTRRGGLDI NPCRVSDLTW MPKPIRLARQ

References

[1]

"Comparative genome sequence analysis of multidrug-resistant Acinetobacter baumannii." Adams M.D., Goglin K., Molyneaux N., Hujer K.M., Lavender H., Jamison J.J., MacDonald I.J., Martin K.M., Russo T., Campagnari A.A., Hujer A.M., Bonomo R.A., Gill S.R. J. Bacteriol. 190:8053-8064(2008) [PubMed: 18931120] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001182 Genomic DNA. Translation: ACJ42097.1.
RefSeq	YP_002320086.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7043290.
GenomeReviews	Gene locus AB57_2746 in contig CP001182_GR.
KEGG	abn:AB57_2746.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00817. [Tree]
InterPro	IPR016428. CN_OxRdtase_NADPH-dep_YqcD. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. [Graphical view]
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMs	TIGR03138. QueF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEF_ACIB5

Accession

Primary (citable) accession number: B7I3J1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	October 13, 2009
This is version 9 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)