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Protein

Chaperone protein DnaJ

Gene

dnaJ

Organism
Acinetobacter baumannii (strain AB0057)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi147Zinc 1UniRule annotation1
Metal bindingi150Zinc 1UniRule annotation1
Metal bindingi164Zinc 2UniRule annotation1
Metal bindingi167Zinc 2UniRule annotation1
Metal bindingi186Zinc 2UniRule annotation1
Metal bindingi189Zinc 2UniRule annotation1
Metal bindingi200Zinc 1UniRule annotation1
Metal bindingi203Zinc 1UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri134 – 212CR-typeUniRule annotationAdd BLAST79

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperone
Biological processDNA replication, Stress response
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein DnaJUniRule annotation
Gene namesi
Name:dnaJUniRule annotation
Ordered Locus Names:AB57_3901
OrganismiAcinetobacter baumannii (strain AB0057)
Taxonomic identifieri480119 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeAcinetobacterAcinetobacter calcoaceticus/baumannii complex

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001376481 – 370Chaperone protein DnaJAdd BLAST370

Interactioni

Subunit structurei

Homodimer.UniRule annotation

GO - Molecular functioni

Structurei

3D structure databases

ProteinModelPortaliB7I2B2.
SMRiB7I2B2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 70JUniRule annotationAdd BLAST66
Repeati147 – 154CXXCXGXG motif8
Repeati164 – 171CXXCXGXG motif8
Repeati186 – 193CXXCXGXG motif8
Repeati200 – 207CXXCXGXG motif8

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi77 – 117Gly-richAdd BLAST41

Domaini

The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.UniRule annotation

Sequence similaritiesi

Belongs to the DnaJ family.UniRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri134 – 212CR-typeUniRule annotationAdd BLAST79

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

HOGENOMiHOG000226717.
KOiK03686.
OMAiDMGGFAD.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
cd10719. DnaJ_zf. 1 hit.
Gene3Di1.10.287.110. 1 hit.
HAMAPiMF_01152. DnaJ. 1 hit.
InterProiView protein in InterPro
IPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
PfamiView protein in Pfam
PF00226. DnaJ. 1 hit.
PF01556. DnaJ_C. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
PRINTSiPR00625. JDOMAIN.
SMARTiView protein in SMART
SM00271. DnaJ. 1 hit.
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
TIGRFAMsiTIGR02349. DnaJ_bact. 1 hit.
PROSITEiView protein in PROSITE
PS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.

Sequencei

Sequence statusi: Complete.

B7I2B2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKRDYYEVL GVSKTASDDE IKKAYRKLAM KYHPDRNPDN AEAEEKFKEA
60 70 80 90 100
SEAYEILSDS EKRSMYDRMG HNAFEGGFGG AGGGFGGFSA EDIFSQFGDI
110 120 130 140 150
FGGAFGGGGR QQRQRRGSDL RYVMELTLEE AVKGVKKTIT FTAPAPCDVC
160 170 180 190 200
DGKGSKNPKD VETCKTCHGS GQVRMQQGFF SVQQTCGTCR GQGKIIKNPC
210 220 230 240 250
HACHGSGVAD RQQTLEVTIP AGVDNGDRVR LSGKGEAIRD GQAGDLYVEV
260 270 280 290 300
VVREHEIFQR DGADLYMDVP VSIADAALGK EIEIPTLEGR VSLKIPEGTQ
310 320 330 340 350
TGKLFRLRGK GVRPVRSSMV GDLLCRIVVE TPVNLTSRQR ELLKELQASF
360 370
DGEDSASSPK KKSFFDRLFD
Length:370
Mass (Da):40,464
Last modified:February 10, 2009 - v1
Checksum:iE48088A8DA2916DD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001182 Genomic DNA. Translation: ACJ42829.1.
RefSeqiWP_001119029.1. NC_011586.1.

Genome annotation databases

EnsemblBacteriaiACJ42829; ACJ42829; AB57_3901.
GeneIDi31349779.
KEGGiabn:AB57_3901.

Similar proteinsi

Entry informationi

Entry nameiDNAJ_ACIB5
AccessioniPrimary (citable) accession number: B7I2B2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: June 7, 2017
This is version 58 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families