B7I0B6 (PRPE_BACC7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bis(5'-nucleosyl)-tetraphosphatase prpE [asymmetrical]
EC=3.6.1.17

Alternative name(s):
Ap4A hydrolase
Diadenosine 5',5'''-P1,P4-tetraphosphate asymmetrical hydrolase
Short name=Diadenosine tetraphosphatase

Gene names

Name:	prpE
Ordered Locus Names:	BCAH187_A1362

Organism

Bacillus cereus (strain AH187) [Complete proteome] [HAMAP]

Taxonomic identifier

405534 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Asymmetrically hydrolyzes Ap4p to yield AMP and ATP By similarity. HAMAP MF_01443
Catalytic activity	P1,P(4)-bis(5'-guanosyl) tetraphosphate + H2O = GTP + GMP. HAMAP MF_01443
Cofactor	Nickel By similarity. HAMAP MF_01443
Sequence similarities	Belongs to the prpE family.

Ontologies

Keywords
Ligand	Nickel
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity Inferred from electronic annotation. Source: EC nickel cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 246	246	Bis(5'-nucleosyl)-tetraphosphatase prpE [asymmetrical] HAMAP MF_01443		PRO_1000145930

Sequences

	Sequence		Length	Mass (Da)
	B7I0B6 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 3868DF986C032BFA	FASTA	246	28,225
	10 20 30 40 50 60 MKYDIIGDIH GCLQEFQNLT EKLGYNWSSG LPVHPDQRKL AFVGDITDRG PHSLRMIEIV 70 80 90 100 110 120 WELVIHKKVA YYAPGNHCNK LYRFFLGRNV TIAHGLETTV AEYEALPSHK QNMIKEKFIT 130 140 150 160 170 180 LYEQSPLYHV LDEKRLLVCH AGIRQDYIGR QDKKVQTFVL YGDITGEKHA DGSPVRRDWA 190 200 210 220 230 240 KEYKGTTWIV YGHTPVKEPR FVNHTVNIDT GAVFGGKLTG LRYPEMEIVS VPSSLPFVPE KFRPIS

References

[1]	"Genome sequence of Bacillus cereus AH187." Dodson R.J., Durkin A.S., Rosovitz M.J., Rasko D.A., Kolsto A.B., Okstad O.A., Ravel J., Sutton G. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AH187.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001177 Genomic DNA. Translation: ACJ77521.1.
RefSeq	YP_002337332.1. NC_011658.1.
3D structure databases
ProteinModelPortal	B7I0B6.
ModBase	Search...
Protein-protein interaction databases
STRING	B7I0B6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000094272; EBBACP00000091824; EBBACG00000094265.
GeneID	7076722.
GenomeReviews	Gene locus BCAH187_A1362 in contig CP001177_GR.
KEGG	bcr:BCAH187_A1362.
PATRIC	18828145. VBIBacCer120511_1547.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000002430.
HOGENOM	HBG724457.
OMA	YVPGNHC.
ProtClustDB	PRK13625.
Family and domain databases
HAMAP	MF_01443. PrpE. [Tree]
InterPro	IPR023937. Bis(5'-nucleosyl)-tetraP_PrpE. IPR004843. Metallo_PEstase_dom. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PRPE_BACC7

Accession

Primary (citable) accession number: B7I0B6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	January 25, 2012
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program