B7HYD0 (B7HYD0_BACC7) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Spermidine synthase HAMAP-Rule MF_00198
EC=2.5.1.16 HAMAP-Rule MF_00198

Alternative name(s):
Putrescine aminopropyltransferase HAMAP-Rule MF_00198
SPDSY HAMAP-Rule MF_00198

Gene names

Name:	speE HAMAP-Rule MF_00198 EMBL ACJ79756.1
Ordered Locus Names:	BCAH187_A5548 EMBL ACJ79756.1

Organism

Bacillus cereus (strain AH187) [Complete proteome] [HAMAP] EMBL ACJ79756.1

Taxonomic identifier

405534 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group › Bacillus cereus

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor By similarity. HAMAP-Rule MF_00198
Catalytic activity	S-adenosylmethioninamine + putrescine = 5'-S-methyl-5'-thioadenosine + spermidine. RuleBase RU003837 HAMAP-Rule MF_00198
Pathway	Amine and polyamine biosynthesis; spermidine biosynthesis; spermidine from putrescine: step 1/1. HAMAP-Rule MF_00198
Subunit structure	Homodimer or homotetramer By similarity. HAMAP-Rule MF_00198
Sequence similarities	Belongs to the spermidine/spermine synthase family. RuleBase RU003836 HAMAP-Rule MF_00198

Ontologies

Keywords
Biological process	Polyamine biosynthesis HAMAP-Rule MF_00198 Spermidine biosynthesis RuleBase RU003837 HAMAP-Rule MF_00198
Molecular function	Transferase RuleBase RU003836 HAMAP-Rule MF_00198 EMBL ACJ79756.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	spermidine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	spermidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	137 – 138	2	S-adenosylmethioninamine binding By similarity HAMAP-Rule MF_00198
Sites
	Active site	155	1	Proton acceptor By similarity HAMAP-Rule MF_00198
	Binding site	31	1	S-adenosylmethioninamine By similarity HAMAP-Rule MF_00198
	Binding site	61	1	Putrescine By similarity HAMAP-Rule MF_00198
	Binding site	86	1	S-adenosylmethioninamine By similarity HAMAP-Rule MF_00198
	Binding site	106	1	S-adenosylmethioninamine By similarity HAMAP-Rule MF_00198
	Binding site	158	1	Putrescine By similarity HAMAP-Rule MF_00198
	Binding site	223	1	Putrescine By similarity HAMAP-Rule MF_00198

Sequences

	Sequence		Length	Mass (Da)
	B7HYD0 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: D4752C642BD883AD	FASTA	275	31,144
	10 20 30 40 50 60 MELWFTEKQT KHFGITARIN RTLHTEQTEF QKLDMVETEE FGNMLILDGM VMTTEKDEFV 70 80 90 100 110 120 YHEMVAHVPL FTHPNPENVL VVGGGDGGVI REVLKHPSVK KATLVEIDGK VIEYSKQYLP 130 140 150 160 170 180 SIAGALDNER VEVKVGDGFL HIAESENEYD VIMVDSTEPV GPAVNLFTKG FYAGISKALK 190 200 210 220 230 240 EDGIFVAQTD NPWFTPELIT TVFKDVKEIF PITRLYTANI PTYPSGLWTF TIGSKKHDPL 250 260 270 EVSEERFHEI ETKYYTKELH NAAFALPKFV GDLIK

References

[1]	"Genome sequence of Bacillus cereus AH187." Dodson R.J., Durkin A.S., Rosovitz M.J., Rasko D.A., Kolsto A.B., Okstad O.A., Ravel J., Sutton G. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AH187 EMBL ACJ79756.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001177 Genomic DNA. Translation: ACJ79756.1.
RefSeq	YP_002341437.1. NC_011658.1.
3D structure databases
ProteinModelPortal	B7HYD0.
SMR	B7HYD0. Positions 2-274.
ModBase	Search...
Protein-protein interaction databases
STRING	405534.BCAH187_A5548.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACJ79756; ACJ79756; BCAH187_A5548.
GeneID	7075725.
KEGG	bcr:BCAH187_A5548.
PATRIC	18836521. VBIBacCer120511_5703.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0421.
HOGENOM	HOG000256146.
KO	K00797.
OMA	ELWYTEK.
ProtClustDB	PRK00811.
Enzyme and pathway databases
BioCyc	BCER405534:GHXM-2591-MONOMER.
UniPathway	UPA00248; UER00314.
Family and domain databases
HAMAP	MF_00198. Spermidine_synth.
InterPro	IPR001045. Spermidine/spermine_synthase. [Graphical view]
PANTHER	PTHR11558. PTHR11558. 1 hit.
Pfam	PF01564. Spermine_synth. 1 hit. [Graphical view]
TIGRFAMs	TIGR00417. speE. 1 hit.
PROSITE	PS01330. SPERMIDINE_SYNTHASE_1. 1 hit. PS51006. SPERMIDINE_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B7HYD0_BACC7

Accession

Primary (citable) accession number: B7HYD0

Entry history

Integrated into UniProtKB/TrEMBL:	February 10, 2009
Last sequence update:	February 10, 2009
Last modified:	May 1, 2013
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)