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B7HSN5 (TRMB_BACC7) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:BCAH187_A4833
OrganismBacillus cereus (strain AH187) [Complete proteome] [HAMAP]
Taxonomic identifier405534 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group

Protein attributes

Sequence length217 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 217217tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_1000136341

Regions

Region191 – 1944Substrate binding By similarity

Sites

Active site1181 By similarity
Binding site441S-adenosyl-L-methionine By similarity
Binding site691S-adenosyl-L-methionine By similarity
Binding site961S-adenosyl-L-methionine By similarity
Binding site1181S-adenosyl-L-methionine By similarity
Binding site1221Substrate By similarity
Binding site1541Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
B7HSN5 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: 3227646A0B193686

FASTA21725,600
        10         20         30         40         50         60 
MRLRHKPYAM DRINEYSHIV IGNPEERAGN WKEVFGNEQP IHIEVGTGRG RFMYDMAKAN 

        70         80         90        100        110        120 
PHINYIGIEK FTSVVVDALD KLIEEELPNL KLINKDAEDL TVFFAKGEID RVYLNFSDPW 

       130        140        150        160        170        180 
PKKRHTKRRL TYKTFLRNYE EVLVEGGEIH FKTDNQGLFE YSLMSMAEYG MLLTYLSLDL 

       190        200        210 
HNSDFEGNIM TEYEEKFSSK GHRIYRVEAK YRTEPMQ 

« Hide

References

[1]"Genome sequence of Bacillus cereus AH187."
Dodson R.J., Durkin A.S., Rosovitz M.J., Rasko D.A., Kolsto A.B., Okstad O.A., Ravel J., Sutton G.
Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AH187.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001177 Genomic DNA. Translation: ACJ79615.1.
RefSeqYP_002340756.1. NC_011658.1.

3D structure databases

ProteinModelPortalB7HSN5.
SMRB7HSN5. Positions 11-213.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING405534.BCAH187_A4833.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACJ79615; ACJ79615; BCAH187_A4833.
GeneID7077780.
KEGGbcr:BCAH187_A4833.
PATRIC18835091. VBIBacCer120511_5014.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000251689.
KOK03439.
OMAKTDDDEL.
OrthoDBEOG6K6VBC.
ProtClustDBPRK00121.

Enzyme and pathway databases

BioCycBCER405534:GHXM-4763-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_BACC7
AccessionPrimary (citable) accession number: B7HSN5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: April 16, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways