B7HNI1 (NAMA_BACC7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADPH dehydrogenase
EC=1.6.99.1

Alternative name(s):
Xenobiotic reductase

Gene names

Name:	namA
Ordered Locus Names:	BCAH187_A2139

Organism

Bacillus cereus (strain AH187) [Complete proteome] [HAMAP]

Taxonomic identifier

405534 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reduction of the double bond of an array of alpha, beta-unsaturated aldehydes and ketones. It also reduces the nitro group of nitroester and nitroaromatic compounds. It could have a role in detoxification processes By similarity. HAMAP MF_01614
Catalytic activity	NADPH + acceptor = NADP+ + reduced acceptor. HAMAP MF_01614
Cofactor	FMN By similarity. HAMAP MF_01614
Subunit structure	Homotetramer By similarity. HAMAP MF_01614
Sequence similarities	Belongs to the NADH:flavin oxidoreductase/NADH oxidase family. NamA subfamily.

Ontologies

Keywords
Ligand	FMN Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	FMN binding Inferred from electronic annotation. Source: InterPro NADPH dehydrogenase activity Inferred from electronic annotation. Source: EC pentaerythritol trinitrate reductase activity Inferred from electronic annotation. Source: EC trichloro-p-hydroquinone reductive dehalogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 345	345	NADPH dehydrogenase HAMAP MF_01614		PRO_1000185862
Regions
	Nucleotide binding	23 – 27	5	FMN By similarity
Sites
	Binding site	28	1	Substrate By similarity
	Binding site	164	1	Substrate By similarity
	Binding site	167	1	Substrate By similarity
	Binding site	215	1	FMN By similarity
	Binding site	308	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7HNI1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: AF0F3C5C3777A944	FASTA	345	38,595
	10 20 30 40 50 60 MNYKLFSPYT IKDVTLKNRI VMSPMCMYSS KNEDGQITNF HLIHYGTRAA GQVGLVMIEA 70 80 90 100 110 120 TAVLPEGRIS NKDLGIWDDS LIEGLHKATT FIHDNGAKAA IQLAHAGRKA ELETDALAPS 130 140 150 160 170 180 AIPFNETMKM PIEMSKHQIK DTVLAFQQAA VRSKQAGFDV IEIHGAHGYL INEFLSPLTN 190 200 210 220 230 240 KRTDEYGGSP ENRYRFLREI IESINEVWNG PLFVRISAND YHPDGLTVQD YVQYTKWMKE 250 260 270 280 290 300 QGVDLIDCSS GAVVPARIDV YPGYQVQYAK HIKEHANIAT GAVGLITTGA QAEQILNNNE 310 320 330 340 ADLIFIGREL LRNPYFSRIA ANELGFELEE PYQYERAPGK ISTNK

References

[1]	"Genome sequence of Bacillus cereus AH187." Dodson R.J., Durkin A.S., Rosovitz M.J., Rasko D.A., Kolsto A.B., Okstad O.A., Ravel J., Sutton G. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AH187.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001177 Genomic DNA. Translation: ACJ78075.1.
RefSeq	YP_002338092.1. NC_011658.1.
3D structure databases
ProteinModelPortal	B7HNI1.
ModBase	Search...
Protein-protein interaction databases
STRING	B7HNI1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000092228; EBBACP00000088169; EBBACG00000092221.
GeneID	7074142.
GenomeReviews	Gene locus BCAH187_A2139 in contig CP001177_GR.
KEGG	bcr:BCAH187_A2139.
PATRIC	18829676. VBIBacCer120511_2311.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000002219.
HOGENOM	HBG583461.
OMA	WVEDGWN.
ProtClustDB	PRK13523.
Family and domain databases
HAMAP	MF_01614. NamA. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR023663. NADPH_DH. IPR001155. OxRdtase_FMN_N. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00724. Oxidored_FMN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAMA_BACC7

Accession

Primary (citable) accession number: B7HNI1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	February 10, 2009
Last modified:	January 25, 2012
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program