Reviewed, UniProtKB/Swiss-Prot B7HND7 (NADE_BACC7)

Names and origin

Protein names

Recommended name:
    NH(3)-dependent NAD(+) synthetase
    EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	BCAH187_A2095

Organism

Bacillus cereus (strain AH187) [Complete proteome] [HAMAP]

Taxonomic identifier

405534 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 272	272	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000118616
Regions
	Nucleotide binding	45 – 52	8	ATP By similarity
Sites
	Active site	47	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7HND7-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 6B3E3FD7DA4EABB5	FASTA	272	30,029
	10 20 30 40 50 60 MTLQEQIMKA LHVQPVIDPK AEIRKRVDFL KDYVKKTGAK GFVLGISGGQ DSTLAGRLAQ 70 80 90 100 110 120 LAVEEIRNEG GNATFIAVRL PYKVQKDEDD AQLALQFIQA DQSVAFDIAS TVDAFSNQYE 130 140 150 160 170 180 NLLGESLTDF NKGNVKARIR MVTQYAIGGQ NGLLVIGTDH AAEAVTGFFT KFGDGGADLL 190 200 210 220 230 240 PLTGLTKRQG RALLQELGAD ERLYLKMPTA DLLDEKPGQA DETELGITYD QLDDYLEGKA 250 260 270 VPTDVAEKIE KRYTVSEHKR QVPASMFDDW WK

References

[1]	"Genome sequence of Bacillus cereus AH187." Dodson R.J., Durkin A.S., Rosovitz M.J., Rasko D.A., Kolsto A.B., Okstad O.A., Ravel J., Sutton G. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001177 Genomic DNA. Translation: ACJ80589.1.
RefSeq	YP_002338048.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7077510.
GenomeReviews	Gene locus BCAH187_A2095 in contig CP001177_GR.
KEGG	bcr:BCAH187_A2095.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00193. [Tree]
InterPro	IPR003694. NAD_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. nadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_BACC7

Accession

Primary (citable) accession number: B7HND7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	June 16, 2009
This is version 6 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)