Reviewed, UniProtKB/Swiss-Prot B7HMX7 (METE_BACC7)

Names and origin

Protein names

Recommended name:
    5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
    EC=2.1.1.14
Alternative name(s):
    Methionine synthase, vitamin-B12 independent isozyme
    Cobalamin-independent methionine synthase

Gene names

Name:	metE
Ordered Locus Names:	BCAH187_A4127

Organism

Bacillus cereus (strain AH187) [Complete proteome] [HAMAP]

Taxonomic identifier

405534 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	762 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity.
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 762	762	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000191192
Sites
	Metal binding	645	1	Zinc By similarity
	Metal binding	647	1	Zinc By similarity
	Metal binding	730	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7HMX7-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: F9AB12473DD67B36	FASTA	762	87,343
	10 20 30 40 50 60 MAIQTSNLGY PRIGLQREWK KTLEAFWSNK IDEEQFLTTM KEIRLKHVKA QQEKGIELIP 70 80 90 100 110 120 IGDFTYYDHV LDTAYMLGFI PSRFSEFTSY LDVYFAMARG SKDHVASEMT KWFNTNYHYI 130 140 150 160 170 180 VPEYEEGLQI SLKDNRPLRL YEEAKQELGV DGKPVILGPY TFLKLAKGYT QEQFATILKQ 190 200 210 220 230 240 LVAPYVQLLS ELHAAGAQII QVDEPIFASL TKEEVQQAKE IYEAIRKEVP NANLLLQTYF 250 260 270 280 290 300 DSVEENYEEI ITFPVSSIGL DFIHGKEGNL HAISKYGFPA DKTLAVGCID GRNIWRADLD 310 320 330 340 350 360 EVLTLFTTLQ KQVQTKNFIV QPSCSLLHTP IDKTEETHLS TELFDALAFA NQKLEELVLI 370 380 390 400 410 420 HSALTQGTES IRNELETYRN VHHTIRSSAA RNREDVKAAR TALKEEDFSR PLPFEKRYEL 430 440 450 460 470 480 QQVALKLPLL PTTTIGSFPQ TTEVRQTRKE WRNGVISNEQ YEQFIEKETE KWIRYQEEIG 490 500 510 520 530 540 LDVLVHGEFE RTDMVEYFGE RLAGFSFTKN GWVQSYGSRC VKPPVIYGDV AFINGMTIKE 550 560 570 580 590 600 TVYAQSLTEK VVKGMLTGPV TILNWSFVRN DIPRKEVSYQ IALALRHEIE LLESSGIRVI 610 620 630 640 650 660 QVDEPALREG MPLKEKDWDA YITWAVQSFL LATSSVANET QIHTHMCYSN FEDIVDAIRA 670 680 690 700 710 720 LDADVISIET SRSHGEFIDT LKHTTYEKGI GLGVYDIHSP RVPSKDEMYK IVEQSLKVCD 730 740 750 760 PKYFWINPDC GLKTRRTEEV IPALEHMVQA AKDARSLLKT NA

References

[1]	"Genome sequence of Bacillus cereus AH187." Dodson R.J., Durkin A.S., Rosovitz M.J., Rasko D.A., Kolsto A.B., Okstad O.A., Ravel J., Sutton G. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001177 Genomic DNA. Translation: ACJ77559.1.
RefSeq	YP_002340061.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7073838.
GenomeReviews	Gene locus BCAH187_A4127 in contig CP001177_GR.
KEGG	bcr:BCAH187_A4127.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00172. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR002629. Methionine_synth. IPR006276. MeTrfase_B12_ind. [Graphical view]
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_BACC7

Accession

Primary (citable) accession number: B7HMX7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	July 28, 2009
This is version 7 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)