Reviewed, UniProtKB/Swiss-Prot B7HMM7 (ILVD_BACC7)

Names and origin

Protein names

Recommended name:
    Dihydroxy-acid dehydratase
      Short name=DAD
    EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	BCAH187_A1969

Organism

Bacillus cereus (strain AH187) [Complete proteome] [HAMAP]

Taxonomic identifier

405534 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	557 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the ilvD/edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 557	557	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_1000190646
Sites
	Metal binding	119	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	192	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	B7HMM7-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 63F605C96DB914AB	FASTA	557	59,975
	10 20 30 40 50 60 MRSDMIKKGF DKAPHRSLLK ATGLKDEDFD KPFIAICNSF IEIIPGHKHL NEFGKLVKEA 70 80 90 100 110 120 VRAAGMVPFE FNTIGVDDGI AMGHIGMRYS LPSREIIADS VETVVNAHWF DGMICIPNCD 130 140 150 160 170 180 KITPGMMMAA LRINIPTVFV SGGPMAAGKT SKGEVVDLSS VFEGVGAYQS GKISEEELKD 190 200 210 220 230 240 IEDHGCPSCG SCSGMFTANS MNCLCEVLGL ALPGNGSILA IDPRREELIK EAAEKLKVLI 250 260 270 280 290 300 ERDIKPRDIV TEEAIDDAFA LDMAMGGSTN TVLHTLALAH EAGLDYDMSR IDAVSRRVPH 310 320 330 340 350 360 LCKVSPASNW HMEDIDRAGG ISAILKEMSR KEGVLHLDRI TATGQTLREN IAHAEIKDKE 370 380 390 400 410 420 VIHSLENPHS EEGGLRILKG NLAKDGAVIK SGATEVKRFE GPCVIFNSQD EALAGIMLGK 430 440 450 460 470 480 VKKGDVVVIR YEGPRGGPGM PEMLAPTSAI AGMGLGADVA LLTDGRFSGA SRGISVGHIS 490 500 510 520 530 540 PEAAAGGTIA LLEQGDIVCI DVEERLLEVR ISDEELDKRK KEWKRPEPKV KTGWLGRYAQ 550 MVTSANTGAV LKVPNFD

References

[1]	"Genome sequence of Bacillus cereus AH187." Dodson R.J., Durkin A.S., Rosovitz M.J., Rasko D.A., Kolsto A.B., Okstad O.A., Ravel J., Sutton G. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001177 Genomic DNA. Translation: ACJ82518.1.
RefSeq	YP_002337923.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7073144.
GenomeReviews	Gene locus BCAH187_A1969 in contig CP001177_GR.
KEGG	bcr:BCAH187_A1969.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00012. [Tree]
InterPro	IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00110. ilvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_BACC7

Accession

Primary (citable) accession number: B7HMM7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	October 13, 2009
This is version 9 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)