Reviewed, UniProtKB/Swiss-Prot B7HJ93 (KHSE_BACC4)

Names and origin

Protein names

Recommended name:
    Homoserine kinase
      Short name=HSK
      Short name=HK
    EC=2.7.1.39

Gene names

Name:	thrB
Ordered Locus Names:	BCB4264_A1972

Organism

Bacillus cereus (strain B4264) [Complete proteome] [HAMAP]

Taxonomic identifier

405532 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	297 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-homoserine = ADP + O-phospho-L-homoserine. HAMAP MF_00384
Pathway	Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5. HAMAP MF_00384
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the GHMP kinase family. Homoserine kinase subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Threonine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphorylation Inferred from electronic annotation. Source: InterPro threonine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP homoserine kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 297	297	Homoserine kinase HAMAP MF_00384		PRO_1000122405
Regions
	Nucleotide binding	82 – 92	11	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	B7HJ93-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: FC33A3EC97DF2BC2	FASTA	297	31,990
	10 20 30 40 50 60 MIPFSVRVPA STANVGPGFD SVGIALSLYL DVVVKEKADK WQVIHSFEES IPADDKNLIV 70 80 90 100 110 120 STACKVCPSI SPHIIEVTSN IPLTRGLGSS ASAIVAGIEL ANQLGNLNLT ADQKVQIATN 130 140 150 160 170 180 FEGHPDNVAA SILGGTVIGA LDGKDVSVVR IESKELGVVS LIPNEELNTD ESRSVLPKMF 190 200 210 220 230 240 PFHEAVKASA ISNVLVAALC QKRWEVVGEM MERDHFHEPY RLELVPLLPS IRKCAKEFGA 250 260 270 280 290 YGTALSGAGP SIFILAPYEK RQEIAGQLAR VFTDMKVCEL EIDHKGIIVN KEEHIGS

References

[1]	"Genome sequence of Bacillus cereus B4264." Dodson R.J., Durkin A.S., Rosovitz M.J., Rasko D.A., Hoffmaster A., Ravel J., Sutton G. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001176 Genomic DNA. Translation: ACK63325.1.
RefSeq	YP_002366689.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7098271.
GenomeReviews	Gene locus BCB4264_A1972 in contig CP001176_GR.
KEGG	bcb:BCB4264_A1972.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00384. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR006203. GHMP_knse_ATP_bd_CS. IPR000870. Homoserine_kin. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF000676. Homoser_kin. 1 hit.
PRINTS	PR00958. HOMSERKINASE.
TIGRFAMs	TIGR00191. thrB. 1 hit.
PROSITE	PS00627. GHMP_KINASES_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KHSE_BACC4

Accession

Primary (citable) accession number: B7HJ93

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	September 22, 2009
This is version 7 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)