B7HGW3 (PRPE_BACC4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bis(5'-nucleosyl)-tetraphosphatase prpE [asymmetrical]
EC=3.6.1.17

Alternative name(s):
Ap4A hydrolase
Diadenosine 5',5'''-P1,P4-tetraphosphate asymmetrical hydrolase
Short name=Diadenosine tetraphosphatase

Gene names

Name:	prpE
Ordered Locus Names:	BCB4264_A1257

Organism

Bacillus cereus (strain B4264) [Complete proteome] [HAMAP]

Taxonomic identifier

405532 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Asymmetrically hydrolyzes Ap4p to yield AMP and ATP By similarity. HAMAP MF_01443
Catalytic activity	P1,P(4)-bis(5'-guanosyl) tetraphosphate + H2O = GTP + GMP. HAMAP MF_01443
Cofactor	Nickel By similarity. HAMAP MF_01443
Sequence similarities	Belongs to the prpE family.

Ontologies

Keywords
Ligand	Nickel
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity Inferred from electronic annotation. Source: EC nickel cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 246	246	Bis(5'-nucleosyl)-tetraphosphatase prpE [asymmetrical] HAMAP MF_01443		PRO_1000145929

Sequences

	Sequence		Length	Mass (Da)
	B7HGW3 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 1A1E92BF2AB4206B	FASTA	246	28,206
	10 20 30 40 50 60 MKYDIIGDIH GCFQEFQNLT EKLGYNWSSG LPVHPDQRKL AFVGDITDRG PHSLRMIEIV 70 80 90 100 110 120 WELVIHKKEA YYAPGNHCNK LYRFFLGRNV TVAHGLETTV AEYEALPSHK QNIIKEKFIT 130 140 150 160 170 180 LYEQSPLYHI LDEKRVIVCH AGIRQDYIGR RDKKVQTFVL YGDITGEKHA DGSPVRRDWA 190 200 210 220 230 240 QEYKGQAWIV YGHTPVAEPR FINQTVNIDT GAVFGGKLTG LRYPEMETIS VPSSLPFVAE KFRPIS

References

[1]	"Genome sequence of Bacillus cereus B4264." Dodson R.J., Durkin A.S., Rosovitz M.J., Rasko D.A., Hoffmaster A., Ravel J., Sutton G. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: B4264.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001176 Genomic DNA. Translation: ACK63456.1.
RefSeq	YP_002365990.1. NC_011725.1.
3D structure databases
ProteinModelPortal	B7HGW3.
ModBase	Search...
Protein-protein interaction databases
STRING	B7HGW3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000109876; EBBACP00000104999; EBBACG00000109869.
GeneID	7098047.
GenomeReviews	Gene locus BCB4264_A1257 in contig CP001176_GR.
KEGG	bcb:BCB4264_A1257.
PATRIC	18874473. VBIBacCer117876_1146.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000002430.
HOGENOM	HBG724457.
OMA	YVPGNHC.
ProtClustDB	PRK13625.
Family and domain databases
HAMAP	MF_01443. PrpE. [Tree]
InterPro	IPR023937. Bis(5'-nucleosyl)-tetraP_PrpE. IPR004843. Metallo_PEstase_dom. [Graphical view]
KO	K01090.
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PRPE_BACC4

Accession

Primary (citable) accession number: B7HGW3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	January 25, 2012
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program