Reviewed, UniProtKB/Swiss-Prot B7HF77 (SYFA_BACC4)

Names and origin

Protein names

Recommended name:
    Phenylalanyl-tRNA synthetase alpha chain
    EC=6.1.1.20
Alternative name(s):
    Phenylalanine--tRNA ligase alpha chain
      Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	BCB4264_A4672

Organism

Bacillus cereus (strain B4264) [Complete proteome] [HAMAP]

Taxonomic identifier

405532 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity. HAMAP MF_00281
Subunit structure	Tetramer of two alpha and two beta chains By similarity. HAMAP MF_00281
Subcellular location	Cytoplasm By similarity HAMAP MF_00281.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	Phenylalanyl-tRNA synthetase alpha chain HAMAP MF_00281		PRO_1000119388
Sites
	Metal binding	256	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7HF77-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: EB4175DBE7B38D1E	FASTA	344	38,990
	10 20 30 40 50 60 MEARLKELKQ KALELIEEAK ELKGLNDVRV AYLGKKGPIT EVLRGMGKLS AEERPRMGAL 70 80 90 100 110 120 VNEVREAIQT RLEDKISNLE KAVIEAKLAT ETIDVTLPGR PVETGCHHPL TAVVEQIEDV 130 140 150 160 170 180 FIGMGYEVAE GTEVEKDYYN FEALNLPKDH PARDMQDTFY ITEETLLRTH TSSVQARTME 190 200 210 220 230 240 NNKEKGPIKI ICPGKVYRRD DDDATHSHQF MQIEGLVIDK NIRMSDLKGT LQVFVKKMFG 250 260 270 280 290 300 EDREIRLRPS FFPFTEPSVE MDISCMMCHG KGCGTCKGTG WIEILGAGMV HPNVLEMAGY 310 320 330 340 DSKEYQGFAF GMGAERIAML KYGVDDIRHF YTNDVRFLQQ FKRA

References

[1]	"Genome sequence of Bacillus cereus B4264." Dodson R.J., Durkin A.S., Rosovitz M.J., Rasko D.A., Hoffmaster A., Ravel J., Sutton G. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001176 Genomic DNA. Translation: ACK61871.1.
RefSeq	YP_002369359.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7100104.
GenomeReviews	Gene locus BCB4264_A4672 in contig CP001176_GR.
KEGG	bcb:BCB4264_A4672.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG284353.
Family and domain databases
HAMAP	MF_00281. Phe_tRNA_synth_alpha1. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II_cons-dom. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_synth_II_N. IPR002319. Phenylalanyl-tRNA_Synthase_acu. IPR010978. tRNA_bd_arm. [Graphical view]
PANTHER	PTHR11538. tRNA-synt_2d. 1 hit.
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
TIGRFAMs	TIGR00468. pheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_BACC4

Accession

Primary (citable) accession number: B7HF77

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	February 9, 2010
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)