B7HED4 (TPIS_BACC4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Triosephosphate isomerase
Short name=TIM
EC=5.3.1.1

Alternative name(s):
Triose-phosphate isomerase

Gene names

Name:	tpiA
Ordered Locus Names:	BCB4264_A5251

Organism

Bacillus cereus (strain B4264) [Complete proteome] [HAMAP]

Taxonomic identifier

405532 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	251 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate = glycerone phosphate. HAMAP MF_00147_B
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_00147_B Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1. HAMAP MF_00147_B
Subunit structure	Homodimer By similarity. HAMAP MF_00147_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00147_B.
Sequence similarities	Belongs to the triosephosphate isomerase family.

Ontologies

Keywords
Biological process	Gluconeogenesis Glycolysis Pentose shunt
Cellular component	Cytoplasm
Molecular function	Isomerase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: UniProtKB-KW glycolysis Inferred from electronic annotation. Source: UniProtKB-KW pentose-phosphate shunt Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triose-phosphate isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 251	251	Triosephosphate isomerase HAMAP MF_00147_B		PRO_1000117997
Sites
	Active site	95	1	Electrophile By similarity
	Active site	167	1	Proton acceptor By similarity
	Binding site	9	1	Substrate By similarity
	Binding site	11	1	Substrate By similarity
Amino acid modifications
	Modified residue	213	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7HED4 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 65066B1BDE8FA601	FASTA	251	26,468
	10 20 30 40 50 60 MRKPIIAGNW KMNKTLSEAV SFVEEVKGQI PAASAVDAVV CSPALFLERL VAATEGTDLQ 70 80 90 100 110 120 VGAQNMHFEK NGAFTGEISP VALSDLKVGY VVLGHSERRE MFAETDESVN KKTIAAFEHG 130 140 150 160 170 180 LTPIVCCGET LEERESGKTF DLVAGQVTKA LAGLTEEQVK ATVIAYEPIW AIGTGKSSSS 190 200 210 220 230 240 ADANEVCAHI RKVVAEAVSP EAAEAVRIQY GGSVKPENIK EYMAQSDIDG ALVGGASLEP 250 ASFLGLLGAV K

References

[1]	"Genome sequence of Bacillus cereus B4264." Dodson R.J., Durkin A.S., Rosovitz M.J., Rasko D.A., Hoffmaster A., Ravel J., Sutton G. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: B4264.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001176 Genomic DNA. Translation: ACK63153.1.
RefSeq	YP_002369908.1. NC_011725.1.
3D structure databases
ProteinModelPortal	B7HED4.
SMR	B7HED4. Positions 2-249.
ModBase	Search...
Protein-protein interaction databases
STRING	B7HED4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000107633; EBBACP00000104618; EBBACG00000107626.
GeneID	7101209.
GenomeReviews	Gene locus BCB4264_A5251 in contig CP001176_GR.
KEGG	bcb:BCB4264_A5251.
PATRIC	18882395. VBIBacCer117876_5076.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000002739.
HOGENOM	HBG708281.
OMA	ISGQWPR.
ProtClustDB	PRK00042.
Family and domain databases
HAMAP	MF_00147_B. TIM_B. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR022896. TrioseP_Isoase_bac/euk. IPR000652. Triosephosphate_isomerase. IPR020861. Triosephosphate_isomerase_AS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01803.
PANTHER	PTHR21139. Triophos_ismrse. 1 hit.
Pfam	PF00121. TIM. 1 hit. [Graphical view]
SUPFAM	SSF51351. Triophos_ismrse. 1 hit.
TIGRFAMs	TIGR00419. Tim. 1 hit.
PROSITE	PS00171. TIM_1. 1 hit. PS51440. TIM_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TPIS_BACC4

Accession

Primary (citable) accession number: B7HED4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	January 25, 2012
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program