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Protein

Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

Gene

trmFO

Organism
Bacillus cereus (strain B4264)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + uracil(54) in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil(54) in tRNA + FAD.UniRule annotation

Cofactori

FADUniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 15FADUniRule annotation6

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

FAD, Flavoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOUniRule annotation (EC:2.1.1.74UniRule annotation)
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferaseUniRule annotation
Folate-dependent tRNA(M-5-U54)-methyltransferaseUniRule annotation
Gene namesi
Name:trmFOUniRule annotation
Ordered Locus Names:BCB4264_A3930
OrganismiBacillus cereus (strain B4264)
Taxonomic identifieri405532 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000007096 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001358881 – 434Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOAdd BLAST434

Structurei

3D structure databases

ProteinModelPortaliB7HDV5.
SMRiB7HDV5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MnmG family. TrmFO subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000252054.
KOiK04094.
OMAiDYLNCPM.

Family and domain databases

Gene3Di3.50.50.60. 3 hits.
HAMAPiMF_01037. TrmFO. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
IPR004417. TrmFO.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B7HDV5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTQVVNVIG AGLAGSEAAY QIAKRGVQVR LYEMRPVRQT PAHHTDKFAE
60 70 80 90 100
LVCSNSLRAN TLTNAVGVIK EEMRLMDSVI IRAADECSVP AGGALAVDRH
110 120 130 140 150
EFAAKVTEYV KNHPNVTVMN EEITDIPEGP TIIATGPLTS PDLSAKLKEL
160 170 180 190 200
TGEDYFYFYD AAAPIVEKDS IDMNKVYLKS RYDKGEAAYL NCPMTEEEFD
210 220 230 240 250
RFYEALIAAE TVPLKEFEKE IFFEGCMPVE VMASRGRQTL VFGPMKPVGL
260 270 280 290 300
EDPKTGKTPY AVVQLRQDDA AGTLYNIVGF QTHLKWGPQK EVLQLIPGLE
310 320 330 340 350
NAEIVRYGVM HRNTFINSPN LLRPTYQYKQ RDDLFFAGQM TGVEGYVESA
360 370 380 390 400
ASGLLAGINA ARLVQGEEPV VLPPVTAMGS MANYITATNA KNFQPMNANF
410 420 430
GLFAPLEKKI KKKAERNEAY AARALETIRN FVNI
Length:434
Mass (Da):47,994
Last modified:February 10, 2009 - v1
Checksum:i51D5FC91AA34AD84
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001176 Genomic DNA. Translation: ACK59412.1.
RefSeqiWP_000213002.1. NC_011725.1.

Genome annotation databases

EnsemblBacteriaiACK59412; ACK59412; BCB4264_A3930.
KEGGibcb:BCB4264_A3930.
PATRICi18879763. VBIBacCer117876_3788.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001176 Genomic DNA. Translation: ACK59412.1.
RefSeqiWP_000213002.1. NC_011725.1.

3D structure databases

ProteinModelPortaliB7HDV5.
SMRiB7HDV5.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACK59412; ACK59412; BCB4264_A3930.
KEGGibcb:BCB4264_A3930.
PATRICi18879763. VBIBacCer117876_3788.

Phylogenomic databases

HOGENOMiHOG000252054.
KOiK04094.
OMAiDYLNCPM.

Family and domain databases

Gene3Di3.50.50.60. 3 hits.
HAMAPiMF_01037. TrmFO. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
IPR004417. TrmFO.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRMFO_BACC4
AccessioniPrimary (citable) accession number: B7HDV5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: November 2, 2016
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.