B7HD62 (SYM_BACC4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionine--tRNA ligase
EC=6.1.1.10

Alternative name(s):
Methionyl-tRNA synthetase
Short name=MetRS

Gene names

Name:	metG
Ordered Locus Names:	BCB4264_A5183

Organism

Bacillus cereus (strain B4264) [Complete proteome] [HAMAP]

Taxonomic identifier

405532 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group › Bacillus cereus

Protein attributes

Sequence length	544 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP-Rule MF_00098
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP-Rule MF_00098
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW methionine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 544	544	Methionine--tRNA ligase HAMAP-Rule MF_00098		PRO_1000199275
Regions
	Motif	10 – 20	11	"HIGH" region HAMAP-Rule MF_00098
	Motif	329 – 333	5	"KMSKS" region HAMAP-Rule MF_00098
Sites
	Metal binding	141	1	Zinc By similarity
	Metal binding	144	1	Zinc By similarity
	Metal binding	153	1	Zinc By similarity
	Metal binding	156	1	Zinc By similarity
	Binding site	332	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7HD62 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 3A15A00F56933052	FASTA	544	62,754
	10 20 30 40 50 60 MSIFIGGAWP YANGSLHLGH IASLLPGDIL ARYYRAKGEN VLYVSGSDCN GTPIAIRAKQ 70 80 90 100 110 120 EGVTAKEIAN KYHEEFQRCF RDLGFTYDCY TRTDSEHHHE TVQKVFLRLL EEGYIYKKTV 130 140 150 160 170 180 EQAYCETCTQ FLPDRYVEGI CPYCHEAARG DQCDACSAIL DPLDLLEKKC KLCGSTPSVE 190 200 210 220 230 240 ETEHFYFALH TFQEQIKKVV EIVKEKGTWR DNAIQLTERY VKEGLQDRAV SRDLPIGVSI 250 260 270 280 290 300 PVKGYEDKKI YVWIEAVAGY YSASKHWAEE TGKDDQEFWN SDAQTYYVHG KDNIPFHSVI 310 320 330 340 350 360 WPAVLLGIGE EAIPRHIVSN EYLTVEKRKL STSKNWAVWV PDILERYDPD SIRYFLTVNA 370 380 390 400 410 420 PENRDTDFSW REFIYSHNSE LLGAYGNFVN RTLKFIEKYY GGIVPKGSIE VELKDKVEGL 430 440 450 460 470 480 YKHVGEAIEQ TKFKVALETI FDAVRFANKY FDEKQPWKQR EDDPVSCEET IYNCVYLIAN 490 500 510 520 530 540 FANLLEPFLP FSSERVRSTL SIIKRNWEPQ NTLPSRIDSV QPLFERIDVK QIEHEVGKLY GAVK

References

[1]	"Genome sequence of Bacillus cereus B4264." Dodson R.J., Durkin A.S., Rosovitz M.J., Rasko D.A., Hoffmaster A., Ravel J., Sutton G. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: B4264.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001176 Genomic DNA. Translation: ACK59077.1.
RefSeq	YP_002369842.1. NC_011725.1.
3D structure databases
ProteinModelPortal	B7HD62.
ModBase	Search...
Protein-protein interaction databases
STRING	405532.BCB4264_A5183.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACK59077; ACK59077; BCB4264_A5183.
GeneID	7098094.
KEGG	bcb:BCB4264_A5183.
PATRIC	18882261. VBIBacCer117876_5009.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0143.
HOGENOM	HOG000200402.
KO	K01874.
OMA	SYYFMGK.
ProtClustDB	PRK12268.
Enzyme and pathway databases
BioCyc	BCER405532:GI1K-378-MONOMER.
Family and domain databases
Gene3D	3.40.50.620. 2 hits.
HAMAP	MF_00098. Met_tRNA_synth_type1.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR023458. Met-tRNA_ligase_1. IPR014758. Met-tRNA_synth. IPR015413. Methionyl/Leucyl_tRNA_Synth. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. [Graphical view]
Pfam	PF08264. Anticodon_1. 1 hit. PF09334. tRNA-synt_1g. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00398. metG. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_BACC4

Accession

Primary (citable) accession number: B7HD62

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	May 1, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program