Reviewed, UniProtKB/Swiss-Prot B7H9G6 (LUXS_BACC4)

Names and origin

Protein names

Recommended name:
    S-ribosylhomocysteine lyase
    EC=4.4.1.21
Alternative name(s):
    Autoinducer-2 production protein luxS
    AI-2 synthesis protein

Gene names

Name:	luxS
Ordered Locus Names:	BCB4264_A4917

Organism

Bacillus cereus (strain B4264) [Complete proteome] [HAMAP]

Taxonomic identifier

405532 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	157 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the synthesis of autoinducer 2 (AI-2) which is secreted by bacteria and is used to communicate both the cell density and the metabolic potential of the environment. The regulation of gene expression in response to changes in cell density is called quorum sensing. Catalyzes the transformation of S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5-dihydroxy-2,3-pentadione (DPD) By similarity.
Catalytic activity	S-(5-deoxy-D-ribos-5-yl)-L-homocysteine = L-homocysteine + (4S)-4,5-dihydroxypentan-2,3-dione. HAMAP MF_00091
Cofactor	Binds 1 iron ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the luxS family.

Ontologies

Keywords
Biological process	Autoinducer synthesis Quorum sensing
Ligand	Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	quorum sensing Inferred from electronic annotation. Source: HAMAP
Molecular function	S-ribosylhomocysteine lyase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 157	157	S-ribosylhomocysteine lyase HAMAP MF_00091		PRO_1000117214
Sites
	Metal binding	54	1	Iron By similarity
	Metal binding	58	1	Iron By similarity
	Metal binding	126	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7H9G6-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 5D2BB8E3B7154793	FASTA	157	17,855
	10 20 30 40 50 60 MPSVESFELD HTIVKAPYVR HCGVHNVGSD GIVNKFDIRF CQPNKQAMKP DVIHTLEHLL 70 80 90 100 110 120 AFNLRKYIDR YPHFDIIDIS PMGCQTGYYL VVSGTPTVRE IIDLLELTLK DAVQITEIPA 130 140 150 ANETQCGQAK LHDLEGAQRL MNFWLSQDKD ELEKVFG

References

[1]	"Genome sequence of Bacillus cereus B4264." Dodson R.J., Durkin A.S., Rosovitz M.J., Rasko D.A., Hoffmaster A., Ravel J., Sutton G. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001176 Genomic DNA. Translation: ACK60187.1.
RefSeq	YP_002369602.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7101171.
GenomeReviews	Gene locus BCB4264_A4917 in contig CP001176_GR.
KEGG	bcb:BCB4264_A4917.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00091. [Tree]
InterPro	IPR003815. S-ribosylhomocysteinase. [Graphical view]
Gene3D	G3DSA:3.30.1360.80. S-ribosylhomocysteinase. 1 hit.
Pfam	PF02664. LuxS. 1 hit. [Graphical view]
PIRSF	PIRSF006160. AI2. 1 hit.
PRINTS	PR01487. LUXSPROTEIN.
ProtoNet	Search...

Entry information

Entry name

LUXS_BACC4

Accession

Primary (citable) accession number: B7H9G6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	October 13, 2009
This is version 8 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)