Reviewed, UniProtKB/Swiss-Prot B7H8Z2 (METE_BACC4)

Names and origin

Protein names

Recommended name:
    5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
    EC=2.1.1.14
Alternative name(s):
    Methionine synthase, vitamin-B12 independent isozyme
    Cobalamin-independent methionine synthase

Gene names

Name:	metE
Ordered Locus Names:	BCB4264_A4108

Organism

Bacillus cereus (strain B4264) [Complete proteome] [HAMAP]

Taxonomic identifier

405532 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	762 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity.
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 762	762	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000191194
Sites
	Metal binding	645	1	Zinc By similarity
	Metal binding	647	1	Zinc By similarity
	Metal binding	730	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7H8Z2-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 2E9B3595AEEE0A04	FASTA	762	87,188
	10 20 30 40 50 60 MAIQTSNLGY PRIGLQREWK KTLEAFWSNK IDEEQFLTTM KEIRLQHVKV QQEKGIELIP 70 80 90 100 110 120 IGDFTYYDHV LDTAYMLGFI PSRFSEFTSY LDVYFAMARG SKDHVASEMT KWFNTNYHYI 130 140 150 160 170 180 VPEYEEGLQI SLKDNRPLRL YEEAKQELGV DGKPVILGPY TFLKLAKGYT QEQFPTILKQ 190 200 210 220 230 240 LVAPYVQLLS ELHTAGAPAI QVDEPIFASL TKKEVQQAKE IYEAIRKEVP NATLLLQTYF 250 260 270 280 290 300 DSVEENYEEI ITFPVSGIGL DFIHGKEGNL NAISKYGFPA DKTLAVGCID GRNIWRADLD 310 320 330 340 350 360 EVLELFTTLQ KQVQTKDIIV QPSCSLLHTP IDKTEETHLS TELFDALAFA NQKLEELVLI 370 380 390 400 410 420 HSALTQGTES ISNELETYRN VHHTIRSSAA RNREDVKAAR TALKEEDFSR PLPFEKRYEL 430 440 450 460 470 480 QQVALELPLL PTTTIGSFPQ TTEVRQTRKE WRNGVISNEQ YEQFIEKETE KWIRYQEEIG 490 500 510 520 530 540 LDVLVHGEFE RTDMVEYFGE RLAGFSFTKN GWVQSYGSRC VKPPVIYGDV AFINGMTIKE 550 560 570 580 590 600 TVYAQSLTEK VVKGMLTGPV TILNWSFVRN DIPRKEVSYQ IALALRHEIE LLESSGIRVI 610 620 630 640 650 660 QVDEPALREG MPLKEKDWDA YITWAVQSFL LATSSVANET QIHTHMCYSN FEDIVDAIRA 670 680 690 700 710 720 LDADVISIET SRSHGEFIDT LKHTTYEKGI GLGVYDIHSP RVPSKDEMYT IVEQSLEVCD 730 740 750 760 PKYFWINPDC GLKTRRTEEV IPALEHMVQA AKDARSLLKT NA

References

[1]	"Genome sequence of Bacillus cereus B4264." Dodson R.J., Durkin A.S., Rosovitz M.J., Rasko D.A., Hoffmaster A., Ravel J., Sutton G. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001176 Genomic DNA. Translation: ACK58828.1.
RefSeq	YP_002368805.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7100798.
GenomeReviews	Gene locus BCB4264_A4108 in contig CP001176_GR.
KEGG	bcb:BCB4264_A4108.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00172. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR002629. Methionine_synth. IPR006276. MeTrfase_B12_ind. [Graphical view]
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_BACC4

Accession

Primary (citable) accession number: B7H8Z2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	October 13, 2009
This is version 9 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)