B7H305 (LSPA_ACIB3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipoprotein signal peptidase
EC=3.4.23.36

Alternative name(s):
Prolipoprotein signal peptidase
Signal peptidase II
Short name=SPase II

Gene names

Name:	lspA
Ordered Locus Names:	ABBFA_003491

Organism

Acinetobacter baumannii (strain AB307-0294) [Complete proteome] [HAMAP]

Taxonomic identifier

557600 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter › Acinetobacter calcoaceticus/baumannii complex

Protein attributes

Sequence length	176 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This protein specifically catalyzes the removal of signal peptides from prolipoproteins By similarity. HAMAP MF_00161
Catalytic activity	Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains. HAMAP MF_00161
Pathway	Protein modification; lipoprotein biosynthesis (signal peptide cleavage). HAMAP MF_00161
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP MF_00161.
Sequence similarities	Belongs to the peptidase A8 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Aspartyl protease Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 176	176	Lipoprotein signal peptidase HAMAP MF_00161		PRO_1000190785
Regions
	Transmembrane	10 – 30	21	Helical; Potential
	Transmembrane	48 – 68	21	Helical; Potential
	Transmembrane	78 – 98	21	Helical; Potential
	Transmembrane	102 – 122	21	Helical; Potential
	Transmembrane	141 – 161	21	Helical; Potential
Sites
	Active site	122	1	By similarity
	Active site	149	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7H305 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: CE10C08C4733E40A	FASTA	176	19,911
	10 20 30 40 50 60 MPNSQAKKGL FQFYPHNLIW LGLSVLAIVL DQWTKWIAST HLNYADPVPV LPFLNWTLLH 70 80 90 100 110 120 NYGAAFSFLS DAGGWQRYFF TSLAGLVSIL FVFWLLRMPK KMVVLPVAIA LILGGALGNL 130 140 150 160 170 IDRITLGYVV DFIHVYYQNH HFPAFNIADS AITLGTILLL IDTFFLEKQR PKNSDA

References

[1]

"Comparative genome sequence analysis of multidrug-resistant Acinetobacter baumannii." Adams M.D., Goglin K., Molyneaux N., Hujer K.M., Lavender H., Jamison J.J., MacDonald I.J., Martin K.M., Russo T., Campagnari A.A., Hujer A.M., Bonomo R.A., Gill S.R. J. Bacteriol. 190:8053-8064(2008) [PubMed: 18931120] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AB307-0294.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001172 Genomic DNA. Translation: ACJ58527.1.
RefSeq	YP_002327363.1. NC_011595.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B7H305.
Protein family/group databases
MEROPS	A08.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7060639.
GenomeReviews	Gene locus ABBFA_003491 in contig CP001172_GR.
KEGG	abb:ABBFA_003491.
PATRIC	20708453. VBIAciBau42682_3373.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG724422.
OMA	MATRNNG.
ProtClustDB	PRK00376.
Family and domain databases
HAMAP	MF_00161. LspA. [Tree]
InterPro	IPR001872. Peptidase_A8. [Graphical view]
KO	K03101.
Pfam	PF01252. Peptidase_A8. 1 hit. [Graphical view]
PRINTS	PR00781. LIPOSIGPTASE.
TIGRFAMs	TIGR00077. LspA. 1 hit.
PROSITE	PS00855. SPASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LSPA_ACIB3

Accession

Primary (citable) accession number: B7H305

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	January 25, 2012
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program