Reviewed, UniProtKB/Swiss-Prot B7H1P9 (AMPA_ACIB3)

Names and origin

Protein names

Recommended name:
    Probable cytosol aminopeptidase
    EC=3.4.11.1
Alternative name(s):
    Leucine aminopeptidase
      Short name=LAP
    Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	ABBFA_003304

Organism

Acinetobacter baumannii (strain AB307-0294) [Complete proteome] [HAMAP]

Taxonomic identifier

557600 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	482 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity.
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181
Cofactor	Binds 2 manganese ions per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP metalloexopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 482	482	Probable cytosol aminopeptidase HAMAP MF_00181		PRO_1000118442
Sites
	Active site	263	1	Potential
	Active site	337	1	Potential
	Metal binding	251	1	Manganese 2 By similarity
	Metal binding	256	1	Manganese 1 By similarity
	Metal binding	256	1	Manganese 2 By similarity
	Metal binding	274	1	Manganese 2 By similarity
	Metal binding	333	1	Manganese 1 By similarity
	Metal binding	335	1	Manganese 1 By similarity
	Metal binding	335	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7H1P9-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: E11507C7B3B71401	FASTA	482	52,177
	10 20 30 40 50 60 MKFTTYTTFP EQTSNESLWI LVDSEQLQSN LNTYQINNLE SILTATQFKA NFNETLPLFG 70 80 90 100 110 120 QLSTQPHSQL LGLGKAAELQ AAKLAKLAQT IIKSAQNKFK HIAIDIAALP VEYHYLFALS 130 140 150 160 170 180 LTQAAYGYDE FKSKKNEFVL QQVDLISSQT SLDENQLALV HAVQSGQSYA RDLGNRPGNI 190 200 210 220 230 240 CFPEYLAEQA LALAAEFPDL LKVTVLNEQQ MADLGMYAFL AVSKGSERPG RIVTLEYQAQ 250 260 270 280 290 300 LEQAPVVLVG KGVTFDTGGI SLKPGLGMDE MKFDMCGAAS VLGTIRALCE ARLPIHVVGA 310 320 330 340 350 360 IAAAENMPSG KATRPGDIVT TMSGQTVEIL NTDAEGRLVL CDTLTYIKRF NPAVVIDIAT 370 380 390 400 410 420 LTGACVVALG KVLSGLFSPD DTLAAELQQA GEQSFDRVWR MPVIDDYQEL LDSPFADIAN 430 440 450 460 470 480 IGGPHGGAIT AACFLERFTR DYRWAHLDVA GTAWLSGSAK GATGRPVPLL MQFLANRVST NG

References

[1]

"Comparative genome sequence analysis of multidrug-resistant Acinetobacter baumannii." Adams M.D., Goglin K., Molyneaux N., Hujer K.M., Lavender H., Jamison J.J., MacDonald I.J., Martin K.M., Russo T., Campagnari A.A., Hujer A.M., Bonomo R.A., Gill S.R. J. Bacteriol. 190:8053-8064(2008) [PubMed: 18931120] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001172 Genomic DNA. Translation: ACJ58036.1.
RefSeq	YP_002327180.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7059091.
GenomeReviews	Gene locus ABBFA_003304 in contig CP001172_GR.
KEGG	abb:ABBFA_003304.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00181. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR008283. Peptidase_M17_N. [Graphical view]
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_ACIB3

Accession

Primary (citable) accession number: B7H1P9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	June 16, 2009
This is version 6 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)