Reviewed, UniProtKB/Swiss-Prot B7H1N2 (MURD_ACIB3)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoylalanine--D-glutamate ligase
    EC=6.3.2.9
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
    D-glutamic acid-adding enzyme

Gene names

Name:	murD
Ordered Locus Names:	ABBFA_003287

Organism

Acinetobacter baumannii (strain AB307-0294) [Complete proteome] [HAMAP]

Taxonomic identifier

557600 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	448 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 448	448	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_1000130819
Regions
	Nucleotide binding	112 – 118	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	B7H1N2-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 9C01945CDA3E63EC	FASTA	448	47,932
	10 20 30 40 50 60 MLIQRGGLKV VAGLGISGVS AVNFLHEQGY QVAVTDSRPT PPGHDQIPAG VKTSFGQLDQ 70 80 90 100 110 120 ELLLQAEEII LSPGLAPQLP EIQAAIAKGI SVVGDIQLLR RATDVPIVAI TGSNAKSTVT 130 140 150 160 170 180 TLIGLMAKDA GKKVAVGGNL GRPALDLLKD QPELLVLELS SFQLETTSHL NAEVAVVLNM 190 200 210 220 230 240 SEDHLDRHGN MLGYHQAKHR IFQGAKKVVF NRDDALSRPL VPDTTPMQSF GLNAPDLNQY 250 260 270 280 290 300 GVLRDADGTL WLARGLQRLI KSSDLYIQGM HNVANALACL ALGEAIGLPM ESMLETLKQF 310 320 330 340 350 360 KGLEHRCEYV KTVHDVRYYN DSKGTNVGAT LAAIDGLGAA IEVKKGKVAL ILGGQGKGQD 370 380 390 400 410 420 FGPLRSSIEK YAKVVVLIGE DAPVIEQAIQ GATKILHAAT LKEAVELCQR ETQAEDVVLL 430 440 SPACASFDMF KSYNDRGQQF VACVNSLV

References

[1]

"Comparative genome sequence analysis of multidrug-resistant Acinetobacter baumannii." Adams M.D., Goglin K., Molyneaux N., Hujer K.M., Lavender H., Jamison J.J., MacDonald I.J., Martin K.M., Russo T., Campagnari A.A., Hujer A.M., Bonomo R.A., Gill S.R. J. Bacteriol. 190:8053-8064(2008) [PubMed: 18931120] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001172 Genomic DNA. Translation: ACJ58865.1.
RefSeq	YP_002327163.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7060407.
GenomeReviews	Gene locus ABBFA_003287 in contig CP001172_GR.
KEGG	abb:ABBFA_003287.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00639. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01087. murD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_ACIB3

Accession

Primary (citable) accession number: B7H1N2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	June 16, 2009
This is version 6 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)