Reviewed, UniProtKB/Swiss-Prot B7H073 (NDK_ACIB3)

Names and origin

Protein names

Recommended name:
    Nucleoside diphosphate kinase
      Short name=NDK
      Short name=NDP kinase
    EC=2.7.4.6
Alternative name(s):
    Nucleoside-2-P kinase

Gene names

Name:	ndk
Ordered Locus Names:	ABBFA_003037

Organism

Acinetobacter baumannii (strain AB307-0294) [Complete proteome] [HAMAP]

Taxonomic identifier

557600 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	143 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate By similarity. HAMAP MF_00451
Catalytic activity	ATP + nucleoside diphosphate = ADP + nucleoside triphosphate. HAMAP MF_00451
Cofactor	Magnesium By similarity. HAMAP MF_00451
Subunit structure	Homotetramer By similarity. HAMAP MF_00451
Subcellular location	Cytoplasm By similarity HAMAP MF_00451.
Sequence similarities	Belongs to the NDK family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	CTP biosynthetic process Inferred from electronic annotation. Source: HAMAP GTP biosynthetic process Inferred from electronic annotation. Source: HAMAP UTP biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside diphosphate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 143	143	Nucleoside diphosphate kinase HAMAP MF_00451		PRO_1000192245
Sites
	Active site	117	1	Pros-phosphohistidine intermediate By similarity
	Binding site	11	1	ATP By similarity
	Binding site	59	1	ATP By similarity
	Binding site	87	1	ATP By similarity
	Binding site	93	1	ATP By similarity
	Binding site	104	1	ATP By similarity
	Binding site	114	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7H073-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 13CBBCF29538FA8D	FASTA	143	15,462
	10 20 30 40 50 60 MAIERTLSIV KPDAVSKNHI GEIFARFEKA GLKIVATKMK HLSQADAEGF YAEHKERGFF 70 80 90 100 110 120 GDLVAFMTSG PVVVSVLEGE NAVLAHREIL GATNPKEAAP GTIRADFAVS IDENAAHGSD 130 140 SVASAEREIA YFFADNEICP RTR

References

[1]

"Comparative genome sequence analysis of multidrug-resistant Acinetobacter baumannii." Adams M.D., Goglin K., Molyneaux N., Hujer K.M., Lavender H., Jamison J.J., MacDonald I.J., Martin K.M., Russo T., Campagnari A.A., Hujer A.M., Bonomo R.A., Gill S.R. J. Bacteriol. 190:8053-8064(2008) [PubMed: 18931120] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001172 Genomic DNA. Translation: ACJ57449.1.
RefSeq	YP_002326923.1.
3D structure databases
SMR	B7H073. Positions 2-143.
ModBase	Search...
Genome annotation databases
GeneID	7059488.
GenomeReviews	Gene locus ABBFA_003037 in contig CP001172_GR.
KEGG	abb:ABBFA_003037.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG445152.
OMA	LTGAITK.
Family and domain databases
HAMAP	MF_00451. NDP_kinase. [Tree]
InterPro	IPR001564. Nuc_diP_kinase_core. [Graphical view]
Gene3D	G3DSA:3.30.70.141. NDK. 1 hit.
PANTHER	PTHR11349. Nuc_diP_kinase_core. 1 hit.
Pfam	PF00334. NDK. 1 hit. [Graphical view]
PRINTS	PR01243. NUCDPKINASE.
SMART	SM00562. NDK. 1 hit. [Graphical view]
PROSITE	PS00469. NDP_KINASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDK_ACIB3

Accession

Primary (citable) accession number: B7H073

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	January 19, 2010
This is version 10 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)