Reviewed, UniProtKB/Swiss-Prot B7GWV6 (SYP_ACIB3)

Names and origin

Protein names

Recommended name:
    Prolyl-tRNA synthetase
    EC=6.1.1.15
Alternative name(s):
    Proline--tRNA ligase
      Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	ABBFA_000643

Organism

Acinetobacter baumannii (strain AB307-0294) [Complete proteome] [HAMAP]

Taxonomic identifier

557600 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	571 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity.
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01569
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP proline-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 571	571	Prolyl-tRNA synthetase HAMAP MF_01569		PRO_1000199341

Sequences

	Sequence		Length	Mass (Da)
	B7GWV6-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: F157F4BEB475EA85	FASTA	571	63,275
	10 20 30 40 50 60 MRASRFLFAT LRETPNDAEV ISHQLMLRAG MIRKLASGLY TWLPMGTRVL KKVDAIVREE 70 80 90 100 110 120 MNRSGAMEVF MPVTQPASLW EESGRYEQYG PELLRFKDRH DNPFVLGPTH EEVITDLARN 130 140 150 160 170 180 ELKSYKQLPV NFYQIQTKFR DEIRPRFGVM RSREFIMKDA YSFHATQESL QETYDVMYDT 190 200 210 220 230 240 YSRIFTRLGL DFRPVQADTG SIGGSASHEF HVLAASGEDD IAFSTESDYA ANVEMAEAVL 250 260 270 280 290 300 VGERAAPTQE FKLVETPNQK TIADVCQFLN ADPKQSVKAL LVQGVADEKG NVPVVALFLR 310 320 330 340 350 360 GDHELNEIKA EKHPLVAAPL AFATEEQLQA FGLTAGFTGP QGLVEKGITV IVDRAASVLS 370 380 390 400 410 420 DFVAGANEAD KHAIGVNWER DAQITEVFDL RNVVEGDPSP DGKGTLQIKR GIEVGHIFQL 430 440 450 460 470 480 GTKYSEALGC KVLGEDGKPF TVTMGCYGIG VTRVVAAAIE QNYDDKGIIW PQAIAPFEIA 490 500 510 520 530 540 IVPMNAHKSP RTLEAAEALY AELQAQGFDV LLDDRNERPG VKFSDLELMG IPHRIVIGEK 550 560 570 GLDAGTFEYK GRRDAEASNL TKEELLAKLA R

References

[1]

"Comparative genome sequence analysis of multidrug-resistant Acinetobacter baumannii." Adams M.D., Goglin K., Molyneaux N., Hujer K.M., Lavender H., Jamison J.J., MacDonald I.J., Martin K.M., Russo T., Campagnari A.A., Hujer A.M., Bonomo R.A., Gill S.R. J. Bacteriol. 190:8053-8064(2008) [PubMed: 18931120] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001172 Genomic DNA. Translation: ACJ56792.1.
RefSeq	YP_002324567.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7057551.
GenomeReviews	Gene locus ABBFA_000643 in contig CP001172_GR.
KEGG	abb:ABBFA_000643.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_01569. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004154. Anticodon_bd. IPR004500. Pro-tRNA-synth_IIa_bac. IPR002316. Pro-tRNA-synth_IIa_cons-reg. IPR007214. YbaK/aa-tRNA-synth-assoc-reg. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF04073. YbaK. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
TIGRFAMs	TIGR00409. proS_fam_II. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_ACIB3

Accession

Primary (citable) accession number: B7GWV6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	July 28, 2009
This is version 7 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)