Reviewed, UniProtKB/Swiss-Prot B7GV69 (PYRE_ACIB3)

Names and origin

Protein names

Recommended name:
    Orotate phosphoribosyltransferase
      Short name=OPRT
      Short name=OPRTase
    EC=2.4.2.10

Gene names

Name:	pyrE
Ordered Locus Names:	ABBFA_000141

Organism

Acinetobacter baumannii (strain AB307-0294) [Complete proteome] [HAMAP]

Taxonomic identifier

557600 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	216 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity.
Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01208
Cofactor	Magnesium By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP MF_01208
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Magnesium
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP orotate phosphoribosyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 216	216	Orotate phosphoribosyltransferase HAMAP MF_01208		PRO_1000138753
Regions
	Region	38 – 39	2	Orotate binding By similarity
	Region	75 – 76	2	5-phosphoribose 1-diphosphate binding By similarity
	Region	128 – 136	9	5-phosphoribose 1-diphosphate binding By similarity
Sites
	Binding site	30	1	5-phosphoribose 1-diphosphate By similarity
	Binding site	102	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	103	1	5-phosphoribose 1-diphosphate By similarity
	Binding site	106	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	108	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	132	1	Orotate By similarity
	Binding site	160	1	Orotate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7GV69-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 654166B5CB773CF9	FASTA	216	23,349
	10 20 30 40 50 60 MTTPVSFHPQ AFIELALSRG VLKFGEFTLK SGRVSPYFFN AGLLNDGEAL SLLAQGYADK 70 80 90 100 110 120 LTQCENVDVI FGPAYKGIPF VAATAVALSQ THNKSVPWGF NRKEAKDHGE GGILVGAAVE 130 140 150 160 170 180 GKKVWIIDDV ITAGTAIREV VTILKNAGAT IAGVLVALDR QERGQGELSA IQEVQKELEI 190 200 210 PVHALITMKD LMDYLEAKGE KEALANMQAY REKYGI

References

[1]

"Comparative genome sequence analysis of multidrug-resistant Acinetobacter baumannii." Adams M.D., Goglin K., Molyneaux N., Hujer K.M., Lavender H., Jamison J.J., MacDonald I.J., Martin K.M., Russo T., Campagnari A.A., Hujer A.M., Bonomo R.A., Gill S.R. J. Bacteriol. 190:8053-8064(2008) [PubMed: 18931120] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001172 Genomic DNA. Translation: ACJ57617.1.
RefSeq	YP_002324082.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7060668.
GenomeReviews	Gene locus ABBFA_000141 in contig CP001172_GR.
KEGG	abb:ABBFA_000141.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_01208. [Tree]
InterPro	IPR004467. Or_phspho_trans. IPR002375. Pr/py_Pribosyl_transf_CS. IPR000836. PRibTrfase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR00336. pyrE. 1 hit.
PROSITE	PS00103. PUR_PYR_PR_TRANSFER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRE_ACIB3

Accession

Primary (citable) accession number: B7GV69

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	September 1, 2009
This is version 7 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)