B7GUP8 (FMT_BIFLS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionyl-tRNA formyltransferase
EC=2.1.2.9

Gene names

Name:	fmt
Ordered Locus Names:	Blon_2133, BLIJ_2210

Organism

Bifidobacterium longum subsp. infantis (strain ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12) [Complete proteome] [HAMAP]

Taxonomic identifier

391904 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium › Bifidobacterium longum › Bifidobacterium longum subsp. infantis

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by: (I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-GTP By similarity. HAMAP-Rule MF_00182
Catalytic activity	10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) = tetrahydrofolate + N-formylmethionyl-tRNA(fMet). HAMAP-Rule MF_00182
Sequence similarities	Belongs to the Fmt family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	translational initiation Inferred from electronic annotation. Source: GOC
Molecular_function	methionyl-tRNA formyltransferase activity Inferred from electronic annotation. Source: HAMAP methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	Methionyl-tRNA formyltransferase HAMAP-Rule MF_00182		PRO_1000190008
Regions
	Region	110 – 113	4	Tetrahydrofolate (THF) binding By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7GUP8 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: A80D765714D38A38	FASTA	328	34,747
	10 20 30 40 50 60 MLKLVFAGTP DVAVPSLKAF ATDPRFDVVG VITRPDAPTG RGRKLTPSPV KAKALELGLP 70 80 90 100 110 120 VIDLKPRSPE FMEALNDLHA DIAAVIAYGN ILPKNVLDAV PMGWYNLHFS NLPKWRGAAP 130 140 150 160 170 180 AQRAIWAGDP TTGADVFKVG EGLDDGPIVA SLTIELTGRE TSGELLDRLA EEGAPMYVDA 190 200 210 220 230 240 LAAVGEGTAT FTAQPAECLE YAHKITVEDA RISWTDEAGA IDRQIRACTP HPGAWTELFA 250 260 270 280 290 300 EGPIADNDGS TAKSLTLHIL AAQPADQSNP NTPADLKPGE LKVGKKNVWV GTGSTPLELI 310 320 QVKAQGKKAM RAADWARGAR LSPAACVR

References

[1]

"The genome sequence of Bifidobacterium longum subsp. infantis reveals adaptations for milk utilization within the infant microbiome." Sela D.A., Chapman J., Adeuya A., Kim J.H., Chen F., Whitehead T.R., Lapidus A., Rokhsar D.S., Lebrilla C.B., German J.B., Price N.P., Richardson P.M., Mills D.A. Proc. Natl. Acad. Sci. U.S.A. 105:18964-18969(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12.

[2]

"Bifidobacteria can protect from enteropathogenic infection through production of acetate." Fukuda S., Toh H., Hase K., Oshima K., Nakanishi Y., Yoshimura K., Tobe T., Clarke J.M., Topping D.L., Suzuki T., Taylor T.D., Itoh K., Kikuchi J., Morita H., Hattori M., Ohno H. Nature 469:543-547(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001095 Genomic DNA. Translation: ACJ53194.1. AP010889 Genomic DNA. Translation: BAJ69787.1.
RefSeq	YP_002323572.1. NC_011593.1. YP_005585957.1. NC_017219.1.
3D structure databases
ProteinModelPortal	B7GUP8.
ModBase	Search...
Protein-protein interaction databases
STRING	391904.Blon_2133.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACJ53194; ACJ53194; Blon_2133. BAJ69787; BAJ69787; BLIJ_2210.
GeneID	12167863. 7054189.
KEGG	bln:Blon_2133. blon:BLIJ_2210.
PATRIC	21125335. VBIBifLon71229_2145.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0223.
HOGENOM	HOG000261177.
KO	K00604.
ProtClustDB	CLSK573224.
Enzyme and pathway databases
BioCyc	BLON391904:GCDR-2203-MONOMER.
Family and domain databases
Gene3D	3.10.25.10. 1 hit. 3.40.50.170. 1 hit.
HAMAP	MF_00182. Formyl_trans.
InterPro	IPR005794. Fmt. IPR005793. Formyl_trans_C. IPR002376. Formyl_transf_N. IPR011034. Formyl_transferase_C-like. IPR015518. Met_tRNA_Form_TA-like. [Graphical view]
PANTHER	PTHR11138. PTHR11138. 1 hit.
Pfam	PF02911. Formyl_trans_C. 1 hit. PF00551. Formyl_trans_N. 1 hit. [Graphical view]
SUPFAM	SSF50486. FMT_C_like. 1 hit. SSF53328. formyl_transf. 1 hit.
TIGRFAMs	TIGR00460. fmt. 1 hit.
PROSITE	PS00373. GART. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FMT_BIFLS

Accession

Primary (citable) accession number: B7GUP8
Secondary accession number(s): E8MMY4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	February 10, 2009
Last modified:	May 29, 2013
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program