B7GTM7 (PPNK_BIFLS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable inorganic polyphosphate/ATP-NAD kinase
Short name=Poly(P)/ATP NAD kinase
EC=2.7.1.23

Gene names

Name:	ppnK
Ordered Locus Names:	Blon_1862, BLIJ_1928

Organism

Bifidobacterium longum subsp. infantis (strain ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12) [Complete proteome] [HAMAP]

Taxonomic identifier

391904 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium › Bifidobacterium longum › Bifidobacterium longum subsp. infantis

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus By similarity. HAMAP-Rule MF_00361
Catalytic activity	ATP + NAD+ = ADP + NADP+. HAMAP-Rule MF_00361
Cofactor	Divalent metal ions By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the NAD kinase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding NAD NADP Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	NAD metabolic process Inferred from electronic annotation. Source: InterPro NADP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ kinase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 338	338	Probable inorganic polyphosphate/ATP-NAD kinase HAMAP-Rule MF_00361		PRO_1000133558

Sequences

	Sequence		Length	Mass (Da)
	B7GTM7 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: ABB5814631F57D22	FASTA	338	36,342
	10 20 30 40 50 60 MAKRNAVVVT HTRLRQTGTV VAEAVSQLRV AGFEVTIIDN TEAPDFGVQP PCVSDDTEIV 70 80 90 100 110 120 VVLGGDGTIL RAAELVHCTQ VPILGVNMGH VGFLAEFESF QIDEAIRRVA THDYSIDERM 130 140 150 160 170 180 IAHVDVWLPG ATKPIEDWAL NDITLERADR GKMVELSIRV DDVEMNSFGA DGVIVSTPTG 190 200 210 220 230 240 STAYAFSAGG PVMWPNVKAL QLIPLAAHAL FARPLIIGSG STFTIDILDD SMSEGWICCD 250 260 270 280 290 300 GRRQRALPQG TRVMVRESRD TLRLARLSGM PFTNRLVSKF DLPVVGWREH ARNEASSQPL 310 320 330 HHGHTFPAAA YAAGVAGDAG VAGTEPDKPG ERDGKAGA

References

[1]

"The genome sequence of Bifidobacterium longum subsp. infantis reveals adaptations for milk utilization within the infant microbiome." Sela D.A., Chapman J., Adeuya A., Kim J.H., Chen F., Whitehead T.R., Lapidus A., Rokhsar D.S., Lebrilla C.B., German J.B., Price N.P., Richardson P.M., Mills D.A. Proc. Natl. Acad. Sci. U.S.A. 105:18964-18969(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12.

[2]

"Bifidobacteria can protect from enteropathogenic infection through production of acetate." Fukuda S., Toh H., Hase K., Oshima K., Nakanishi Y., Yoshimura K., Tobe T., Clarke J.M., Topping D.L., Suzuki T., Taylor T.D., Itoh K., Kikuchi J., Morita H., Hattori M., Ohno H. Nature 469:543-547(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001095 Genomic DNA. Translation: ACJ52936.1. AP010889 Genomic DNA. Translation: BAJ69507.1.
RefSeq	YP_002323314.1. NC_011593.1. YP_005585677.1. NC_017219.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	391904.Blon_1862.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACJ52936; ACJ52936; Blon_1862. BAJ69507; BAJ69507; BLIJ_1928.
GeneID	12167581. 7055294.
KEGG	bln:Blon_1862. blon:BLIJ_1928.
PATRIC	21124772. VBIBifLon71229_1866.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0061.
HOGENOM	HOG000227223.
KO	K00858.
ProtClustDB	PRK03372.
Family and domain databases
Gene3D	2.60.200.30. 1 hit. 3.40.50.10330. 1 hit.
HAMAP	MF_00361. NAD_kinase.
InterPro	IPR017438. ATP-NAD_kinase_dom_1. IPR016064. ATP-NAD_kinase_PpnK-typ. IPR017437. ATP-NAD_kinase_PpnK-typ_all-b. IPR002504. PolyP/ATP_NADK_prd. [Graphical view]
PANTHER	PTHR20275. PTHR20275. 1 hit.
Pfam	PF01513. NAD_kinase. 1 hit. [Graphical view]
SUPFAM	SSF111331. ATP-NAD_kinase_PpnK-typ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PPNK_BIFLS

Accession

Primary (citable) accession number: B7GTM7
Secondary accession number(s): E8MLT0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	February 10, 2009
Last modified:	May 1, 2013
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program