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Protein

NAD kinase

Gene

nadK

Organism
Bifidobacterium longum subsp. infantis (strain ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.UniRule annotation

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei66 – 661Proton acceptorUniRule annotation
Binding sitei71 – 711NADUniRule annotation
Binding sitei152 – 1521NADUniRule annotation
Binding sitei171 – 1711NADUniRule annotation
Binding sitei206 – 2061NAD; via carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi66 – 672NADUniRule annotation
Nucleotide bindingi141 – 1422NADUniRule annotation
Nucleotide bindingi182 – 1876NADUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciBLON391904:GCDR-1931-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinaseUniRule annotation (EC:2.7.1.23UniRule annotation)
Alternative name(s):
ATP-dependent NAD kinaseUniRule annotation
Gene namesi
Name:nadKUniRule annotation
Ordered Locus Names:Blon_1862, BLIJ_1928
OrganismiBifidobacterium longum subsp. infantis (strain ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12)
Taxonomic identifieri391904 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaBifidobacterialesBifidobacteriaceaeBifidobacterium
ProteomesiUP000001360 Componenti: Chromosome UP000007256 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 338338NAD kinasePRO_1000133558Add
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000227223.
KOiK00858.
OMAiGVLWCDG.
OrthoDBiEOG6PZXDR.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR016064. NAD/diacylglycerol_kinase.
IPR002504. NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.

Sequencei

Sequence statusi: Complete.

B7GTM7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKRNAVVVT HTRLRQTGTV VAEAVSQLRV AGFEVTIIDN TEAPDFGVQP
60 70 80 90 100
PCVSDDTEIV VVLGGDGTIL RAAELVHCTQ VPILGVNMGH VGFLAEFESF
110 120 130 140 150
QIDEAIRRVA THDYSIDERM IAHVDVWLPG ATKPIEDWAL NDITLERADR
160 170 180 190 200
GKMVELSIRV DDVEMNSFGA DGVIVSTPTG STAYAFSAGG PVMWPNVKAL
210 220 230 240 250
QLIPLAAHAL FARPLIIGSG STFTIDILDD SMSEGWICCD GRRQRALPQG
260 270 280 290 300
TRVMVRESRD TLRLARLSGM PFTNRLVSKF DLPVVGWREH ARNEASSQPL
310 320 330
HHGHTFPAAA YAAGVAGDAG VAGTEPDKPG ERDGKAGA
Length:338
Mass (Da):36,342
Last modified:February 10, 2009 - v1
Checksum:iABB5814631F57D22
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001095 Genomic DNA. Translation: ACJ52936.1.
AP010889 Genomic DNA. Translation: BAJ69507.1.
RefSeqiWP_012578151.1. NC_017219.1.
YP_002323314.1. NC_011593.1.
YP_005585677.1. NC_017219.1.

Genome annotation databases

EnsemblBacteriaiACJ52936; ACJ52936; Blon_1862.
BAJ69507; BAJ69507; BLIJ_1928.
KEGGibln:Blon_1862.
blon:BLIJ_1928.
PATRICi21124772. VBIBifLon71229_1866.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001095 Genomic DNA. Translation: ACJ52936.1.
AP010889 Genomic DNA. Translation: BAJ69507.1.
RefSeqiWP_012578151.1. NC_017219.1.
YP_002323314.1. NC_011593.1.
YP_005585677.1. NC_017219.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACJ52936; ACJ52936; Blon_1862.
BAJ69507; BAJ69507; BLIJ_1928.
KEGGibln:Blon_1862.
blon:BLIJ_1928.
PATRICi21124772. VBIBifLon71229_1866.

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000227223.
KOiK00858.
OMAiGVLWCDG.
OrthoDBiEOG6PZXDR.

Enzyme and pathway databases

BioCyciBLON391904:GCDR-1931-MONOMER.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR016064. NAD/diacylglycerol_kinase.
IPR002504. NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Bifidobacterium longum subsp. infantis reveals adaptations for milk utilization within the infant microbiome."
    Sela D.A., Chapman J., Adeuya A., Kim J.H., Chen F., Whitehead T.R., Lapidus A., Rokhsar D.S., Lebrilla C.B., German J.B., Price N.P., Richardson P.M., Mills D.A.
    Proc. Natl. Acad. Sci. U.S.A. 105:18964-18969(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12.

Entry informationi

Entry nameiNADK_BIFLS
AccessioniPrimary (citable) accession number: B7GTM7
Secondary accession number(s): E8MLT0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 10, 2009
Last modified: June 24, 2015
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.