B7GQU7 (PGK_BIFLS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoglycerate kinase
EC=2.7.2.3

Gene names

Name:	pgk
Ordered Locus Names:	Blon_1087, BLIJ_1111

Organism

Bifidobacterium longum subsp. infantis (strain ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12) [Complete proteome] [HAMAP]

Taxonomic identifier

391904 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium › Bifidobacterium longum › Bifidobacterium longum subsp. infantis

Protein attributes

Sequence length	401 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate. HAMAP-Rule MF_00145
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. HAMAP-Rule MF_00145
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00145.
Sequence similarities	Belongs to the phosphoglycerate kinase family.
Sequence caution	The sequence BAJ68699.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoglycerate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 401	401	Phosphoglycerate kinase HAMAP-Rule MF_00145		PRO_1000192802
Regions
	Nucleotide binding	358 – 361	4	ATP By similarity
	Region	20 – 22	3	Substrate binding By similarity
	Region	58 – 61	4	Substrate binding By similarity
Sites
	Binding site	35	1	Substrate By similarity
	Binding site	117	1	Substrate By similarity
	Binding site	154	1	Substrate By similarity
	Binding site	204	1	ATP By similarity
	Binding site	298	1	ATP; via carbonyl oxygen By similarity
	Binding site	329	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7GQU7 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: E1000743D5E0C47A	FASTA	401	41,896
	10 20 30 40 50 60 MKTLKDLGDL KGKRVLVRAD FNVPLDGTTI TDDGRIKAAL PTIKTLREEG AKVILMAHLG 70 80 90 100 110 120 RPKGKVVPEL SLAPVAARLG ELLGTNVPLA KDTYGEDAQA KVAAMNDGDV VLLENVRFNP 130 140 150 160 170 180 EETSKDADER AAYAKKIAAL GEAFVSDGFG VVHRAQGSNY DVAADLPAAA GLLVEKEVKA 190 200 210 220 230 240 LSKATENPER PFTVVLGGSK VSDKLGVIEN LLDKANRLVI GGGMVFTFLK AKGYEVGTSL 250 260 270 280 290 300 LEEDQLEKVK GYIETAEKNG VELVLPTDVV VNAGFPAGDT PVAPEVVAAD AIPADKMGLD 310 320 330 340 350 360 IGPESQKLFH DKIVDSKTVV WNGPMGVFEV PEFAAGTKAV AQGLVDATAA GAFTIVGGGD 370 380 390 400 SASAVRNLGF PEDGFSHIST GGGASLEFLE GKELPGLKVL E

References

[1]

"The genome sequence of Bifidobacterium longum subsp. infantis reveals adaptations for milk utilization within the infant microbiome." Sela D.A., Chapman J., Adeuya A., Kim J.H., Chen F., Whitehead T.R., Lapidus A., Rokhsar D.S., Lebrilla C.B., German J.B., Price N.P., Richardson P.M., Mills D.A. Proc. Natl. Acad. Sci. U.S.A. 105:18964-18969(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12.

[2]

"Bifidobacteria can protect from enteropathogenic infection through production of acetate." Fukuda S., Toh H., Hase K., Oshima K., Nakanishi Y., Yoshimura K., Tobe T., Clarke J.M., Topping D.L., Suzuki T., Taylor T.D., Itoh K., Kikuchi J., Morita H., Hattori M., Ohno H. Nature 469:543-547(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001095 Genomic DNA. Translation: ACJ52177.1. AP010889 Genomic DNA. Translation: BAJ68699.1. Different initiation.
RefSeq	YP_002322555.1. NC_011593.1. YP_005584869.1. NC_017219.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	391904.Blon_1087.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACJ52177; ACJ52177; Blon_1087. BAJ68699; BAJ68699; BLIJ_1111.
GeneID	12168032. 7054705.
KEGG	bln:Blon_1087. blon:BLIJ_1111.
PATRIC	21123128. VBIBifLon71229_1082.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0126.
HOGENOM	HOG000227108.
KO	K00927.
ProtClustDB	PRK00073.
Enzyme and pathway databases
UniPathway	UPA00109; UER00185.
Family and domain databases
Gene3D	3.40.50.1260. 1 hit. 3.40.50.1270. 1 hit.
HAMAP	MF_00145. Phosphoglyc_kinase.
InterPro	IPR001576. Phosphoglycerate_kinase. IPR015901. Phosphoglycerate_kinase_C. IPR015911. Phosphoglycerate_kinase_CS. IPR015824. Phosphoglycerate_kinase_N. [Graphical view]
PANTHER	PTHR11406. PTHR11406. 1 hit.
Pfam	PF00162. PGK. 1 hit. [Graphical view]
PIRSF	PIRSF000724. Pgk. 1 hit.
PRINTS	PR00477. PHGLYCKINASE.
SUPFAM	SSF53748. PGK. 1 hit.
PROSITE	PS00111. PGLYCERATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGK_BIFLS

Accession

Primary (citable) accession number: B7GQU7
Secondary accession number(s): E8MJH2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	February 10, 2009
Last modified:	May 1, 2013
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program