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B7GQ76 (MURD_BIFLS) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
UDP-N-acetylmuramoylalanine--D-glutamate ligase
EC=6.3.2.9
Alternative name(s):
D-glutamic acid-adding enzyme
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
Gene names
Name:murD
Ordered Locus Names:Blon_0854, BLIJ_0871
OrganismBifidobacterium longum subsp. infantis (strain ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12) [Complete proteome] [HAMAP]
Taxonomic identifier391904 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium ›

Protein attributes

Sequence length481 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity. HAMAP-Rule MF_00639

Catalytic activity

ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP-Rule MF_00639

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00639

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the MurCDEF family.

Sequence caution

The sequence BAJ68463.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 481481UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP-Rule MF_00639
PRO_1000147395

Regions

Nucleotide binding108 – 1147ATP Potential

Sequences

Sequence LengthMass (Da)Tools
B7GQ76 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: 8882581CDDAA6BD1

FASTA48151,034
        10         20         30         40         50         60 
MQVADKTVVI AGLGVSGTSL AEVLRERGAH VIGVDERKPE ADLRSFDDVD WDHVDYVMSS 

        70         80         90        100        110        120 
PVFNPRTPFV LEAQRRGIPV MSEVEFAWQL RVNNERTGTP APWIGITGTN GKTSTTEMTS 

       130        140        150        160        170        180 
EMLTACGLDA PTAGNIASGD MSMSLSRCAT NPQHDVLCVE LSSFQLHFTD SLALDCAAIT 

       190        200        210        220        230        240 
NIADDHLDWH GGRENYAADK SKVFHNAKHA IVYNAQDAKV SELAAEAQTA EGCRKVGFTL 

       250        260        270        280        290        300 
EAPQAGQIGI EDGWIVDRSG VAGGAVGEPV RLAAITDFTH LAEPDGSLYP HLVADALTAL 

       310        320        330        340        350        360 
ALVLGLGADR DTALKALTSF KPGGHRIETV AEAVVEGGSV RFVDDSKATN GHAARASLSS 

       370        380        390        400        410        420 
FPAKSVIWIA GGLAKGSRFE DLVKDQAHTI KAAVIIGKDQ QPMIEAFASQ APDIPVTIID 

       430        440        450        460        470        480 
PEDNDTVMDR AVEACGTYAA AGDIVLMAPA CASMDQFKSY ADRGNRFAAA AKTWSEVHGL 


H

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References

[1]"The genome sequence of Bifidobacterium longum subsp. infantis reveals adaptations for milk utilization within the infant microbiome."
Sela D.A., Chapman J., Adeuya A., Kim J.H., Chen F., Whitehead T.R., Lapidus A., Rokhsar D.S., Lebrilla C.B., German J.B., Price N.P., Richardson P.M., Mills D.A.
Proc. Natl. Acad. Sci. U.S.A. 105:18964-18969(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12.
[2]"Bifidobacteria can protect from enteropathogenic infection through production of acetate."
Fukuda S., Toh H., Hase K., Oshima K., Nakanishi Y., Yoshimura K., Tobe T., Clarke J.M., Topping D.L., Suzuki T., Taylor T.D., Itoh K., Kikuchi J., Morita H., Hattori M., Ohno H.
Nature 469:543-547(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001095 Genomic DNA. Translation: ACJ51956.1.
AP010889 Genomic DNA. Translation: BAJ68463.1. Different initiation.
RefSeqYP_002322334.1. NC_011593.1.
YP_005584633.1. NC_017219.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRING391904.Blon_0854.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACJ51956; ACJ51956; Blon_0854.
BAJ68463; BAJ68463; BLIJ_0871.
GeneID12166448.
7055374.
KEGGbln:Blon_0854.
blon:BLIJ_0871.
PATRIC21122666. VBIBifLon71229_0855.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0771.
HOGENOMHOG000049429.
KOK01925.
ProtClustDBCLSK573012.

Enzyme and pathway databases

UniPathwayUPA00219.

Family and domain databases

Gene3D3.40.1190.10. 1 hit.
3.40.50.720. 1 hit.
3.90.190.20. 1 hit.
HAMAPMF_00639. MurD.
InterProIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR016040. NAD(P)-bd_dom.
IPR005762. UDP-N-AcMur-Glu_ligase.
[Graphical view]
PANTHERPTHR23135:SF2. PTHR23135:SF2. 1 hit.
PfamPF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMSSF53244. Mur_ligase_C. 1 hit.
SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMsTIGR01087. murD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMURD_BIFLS
AccessionPrimary (citable) accession number: B7GQ76
Secondary accession number(s): E8MR38
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 10, 2009
Last modified: May 1, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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